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Frederik Larsen

Identification and tissue distribution by in silico analysis and RT-qPCR expression profiling of chicken c-type lectin-like domain containing proteins

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C-type lectin-like domain containing proteins (CTLDcps) mainly bind carbohydrate-based ligands but also other ligands, e.g. lipids and inorganic compounds, and are involved in several biological processes e.g. cell adhesion, cell-cell interactions, and pathogen recognition. Pathogen recognition by myeloid cells, e.g. dendritic cells (DCs), can be facilitated through cell-surface expressed CTLDcps. Cell-surfaced expressed CTLDcps have been exploited in vaccine design for specific targeting of human and mouse DCs using antibodies. In recent years, however, DC targeting using carbohydrate-based vaccines has gained interest. Carbohydrate-mediated DC targeting in vaccine designs offer some advantages over antibody in e.g. limited immunogenicity and possibility of multivalent adjustment. In chicken, however, only a few CTLDcps have been identified including tissue distribution determination. In order to utilize the potential of chicken CTLDcps (chCTLDcps) to target DCs, we searched the chicken GRCg6a genome assembly for chCTLDcps using the BLEC1 CTLD. We identified 17 novel chCTLDcps distributed on chromosome (chr) 1, 16, 30, and Z, confirmed by RT-qPCR to be expressed, differentially, in various chicken tissues. Phylogenetic analysis and reciprocal blast search suggested the identified chCTLDcps on chr 1 and 16 to be part of an avian- specific expansion of chCTLDcps. Synteny analysis for the genomic region on chr 30 containing chCLEC17AL-A and chCLEC17AL-B revealed synteny between birds and alligator, but not in turtle, lizard, and human.. This specific gene evolution was supported by phylogenetic analysis, demonstrating that gene lineages to chCLEC17AL-A and -B on one side, and hCLEC17A on the other side, evolved separately from a common ancestral gene. In conclusion, we found and confirmed the expression of several novel chCTLDcps. Future determination of DC-specific expression of the chCTLDcps might prove useful in chicken vaccinology.
Original languageEnglish
Publication year4 Sep 2018
Publication statusPublished - 4 Sep 2018
EventXVth Avian Immunology Research Group (AIRG) Meeting 2018 - St. Cathrines College, Oxford, United Kingdom
Duration: 5 Sep 201814 Sep 2018
Conference number: 15
http://airg.zoo.ox.ac.uk/

Conference

ConferenceXVth Avian Immunology Research Group (AIRG) Meeting 2018
Number15
LocationSt. Cathrines College
CountryUnited Kingdom
CityOxford
Period05/09/201814/09/2018
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