Aarhus University Seal / Aarhus Universitets segl

Finn Skou Pedersen

Spatial and temporal changes in the morphology of preosteoblastic cells seeded on microstructured tantalum surfaces

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Interdisciplinary Nanoscience Center
  • Department of Physics and Astronomy
  • Department of Molecular Biology
It has been widely reported that surface morphology on the micrometer scale affects cell function as well as cell shape. In this study, we have systematically compared the influence of 13 topographically micropatterned tantalum surfaces on the temporal development of morphology, including spreading, and length of preosteoblastic cells (MC3T3-E1). Cells were examined after 0.5, 1, 4, and 24 h on different Ta microstructures with vertical dimensions (heights) of 0.25 and 1.6 mum. Cell morphologies depended upon the underlying surface topography, and the length and spreading of cells varied as a function of time with regard to the two-dimensional pattern and vertical dimension of the structure. Microstructures of parallel grooves/ridges caused elongated cell growth after 1 and 4 h in comparison to a flat, nonstructured, reference surface. For microstructures consisting of pillars, cell spreading was found to depend on the distance between the pillars with one specific pillar structure exhibiting a decreased spreading combined with a radical change in morphology of the cells. Interestingly, this morphology on the particular pillar structure was associated with a markedly different distribution of the actin cytoskeleton. Our results provide a basis for further work toward topographical guiding of cell function. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008.
Original languageEnglish
JournalJournal of Biomedical Materials Research. Part A
Pages (from-to)885 - 894
Number of pages10
Publication statusPublished - 2009

See relations at Aarhus University Citationformats

ID: 11813911