Esben Thyssen Vestergaard

Ghrelin does not directly stimulate secretion of glucagon-like peptide-1

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Ghrelin does not directly stimulate secretion of glucagon-like peptide-1. / Jepsen, Sara Lind; Vestergaard, Esben Thyssen; Larraufie, Pierre; Gribble, Fiona Mary; Reimann, Frank; Jørgensen, Jens Otto Lunde; Holst, Jens Juul; Kuhre, Rune Ehrenreich.

In: The Journal of clinical endocrinology and metabolism, Vol. 105, No. 1, dgz046, 01.01.2020, p. 266-275.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Jepsen, SL, Vestergaard, ET, Larraufie, P, Gribble, FM, Reimann, F, Jørgensen, JOL, Holst, JJ & Kuhre, RE 2020, 'Ghrelin does not directly stimulate secretion of glucagon-like peptide-1', The Journal of clinical endocrinology and metabolism, vol. 105, no. 1, dgz046, pp. 266-275. https://doi.org/10.1210/clinem/dgz046

APA

Jepsen, S. L., Vestergaard, E. T., Larraufie, P., Gribble, F. M., Reimann, F., Jørgensen, J. O. L., Holst, J. J., & Kuhre, R. E. (2020). Ghrelin does not directly stimulate secretion of glucagon-like peptide-1. The Journal of clinical endocrinology and metabolism, 105(1), 266-275. [dgz046]. https://doi.org/10.1210/clinem/dgz046

CBE

Jepsen SL, Vestergaard ET, Larraufie P, Gribble FM, Reimann F, Jørgensen JOL, Holst JJ, Kuhre RE. 2020. Ghrelin does not directly stimulate secretion of glucagon-like peptide-1. The Journal of clinical endocrinology and metabolism. 105(1):266-275. https://doi.org/10.1210/clinem/dgz046

MLA

Jepsen, Sara Lind et al. "Ghrelin does not directly stimulate secretion of glucagon-like peptide-1". The Journal of clinical endocrinology and metabolism. 2020, 105(1). 266-275. https://doi.org/10.1210/clinem/dgz046

Vancouver

Jepsen SL, Vestergaard ET, Larraufie P, Gribble FM, Reimann F, Jørgensen JOL et al. Ghrelin does not directly stimulate secretion of glucagon-like peptide-1. The Journal of clinical endocrinology and metabolism. 2020 Jan 1;105(1):266-275. dgz046. https://doi.org/10.1210/clinem/dgz046

Author

Jepsen, Sara Lind ; Vestergaard, Esben Thyssen ; Larraufie, Pierre ; Gribble, Fiona Mary ; Reimann, Frank ; Jørgensen, Jens Otto Lunde ; Holst, Jens Juul ; Kuhre, Rune Ehrenreich. / Ghrelin does not directly stimulate secretion of glucagon-like peptide-1. In: The Journal of clinical endocrinology and metabolism. 2020 ; Vol. 105, No. 1. pp. 266-275.

Bibtex

@article{082b8f4e3d64429ba8f6d3e77014d8f8,
title = "Ghrelin does not directly stimulate secretion of glucagon-like peptide-1",
abstract = "CONTEXT: The gastrointestinal hormone ghrelin stimulates growth hormone secretion and appetite, but recent studies indicate that ghrelin also stimulates the secretion of the appetite-inhibiting and insulinotropic hormone glucagon-like peptide-1 (GLP-1). OBJECTIVE: To investigate the putative effect of ghrelin on GLP-1 secretion in vivo and in vitro. SUBJECTS AND METHODS: A randomized placebo-controlled crossover study was performed in eight hypopituitary subjects. Ghrelin or saline was infused intravenously (1 pmol/min × kg) after collection of baseline sample (0 min), and blood was subsequently collected at time 30, 60, 90, and 120 minutes. Mouse small intestine was perfused (n = 6) and GLP-1 output from perfused mouse small intestine was investigated in response to vascular ghrelin administration in the presence and absence of a simultaneous luminal glucose stimulus. Ghrelin receptor expression was quantified in human (n = 11) and mouse L-cells (n = 3) by RNA sequencing and RT-qPCR, respectively. RESULTS: Ghrelin did not affect GLP-1 secretion in humans (area under the curve [AUC; 0-120 min]: ghrelin infusion = 1.37 ± 0.05 min × nmol vs. saline infusion = 1.40 ± 0.06 min × nmol [P = 0.63]), but induced peripheral insulin resistance. Likewise, ghrelin did not stimulate GLP-1 secretion from the perfused mouse small intestine model (mean outputs during baseline/ghrelin infusion = 19.3 ± 1.6/25.5 ± 2.0 fmol/min, n = 6, P = 0.16), whereas glucose-dependent insulinotropic polypeptide administration, used as a positive control, doubled GLP-1 secretion (P < 0.001). Intraluminal glucose increased GLP-1 secretion by 4-fold (P < 0.001), which was not potentiated by ghrelin. Finally, gene expression of the ghrelin receptor was undetectable in mouse L-cells and marginal in human L-cells. CONCLUSIONS: Ghrelin does not interact directly with the L-cell and does not directly affect GLP-1 secretion.",
keywords = "CELLS, GLP-1 SECRETION, GLUCOSE-ABSORPTION, HORMONES, INSULIN-SECRETION, MEAL, MECHANISMS, RECEPTOR, SURGERY, WEIGHT",
author = "Jepsen, {Sara Lind} and Vestergaard, {Esben Thyssen} and Pierre Larraufie and Gribble, {Fiona Mary} and Frank Reimann and J{\o}rgensen, {Jens Otto Lunde} and Holst, {Jens Juul} and Kuhre, {Rune Ehrenreich}",
note = "{\textcopyright} Endocrine Society 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.",
year = "2020",
month = jan,
day = "1",
doi = "10.1210/clinem/dgz046",
language = "English",
volume = "105",
pages = "266--275",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Ghrelin does not directly stimulate secretion of glucagon-like peptide-1

AU - Jepsen, Sara Lind

AU - Vestergaard, Esben Thyssen

AU - Larraufie, Pierre

AU - Gribble, Fiona Mary

AU - Reimann, Frank

AU - Jørgensen, Jens Otto Lunde

AU - Holst, Jens Juul

AU - Kuhre, Rune Ehrenreich

N1 - © Endocrine Society 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - CONTEXT: The gastrointestinal hormone ghrelin stimulates growth hormone secretion and appetite, but recent studies indicate that ghrelin also stimulates the secretion of the appetite-inhibiting and insulinotropic hormone glucagon-like peptide-1 (GLP-1). OBJECTIVE: To investigate the putative effect of ghrelin on GLP-1 secretion in vivo and in vitro. SUBJECTS AND METHODS: A randomized placebo-controlled crossover study was performed in eight hypopituitary subjects. Ghrelin or saline was infused intravenously (1 pmol/min × kg) after collection of baseline sample (0 min), and blood was subsequently collected at time 30, 60, 90, and 120 minutes. Mouse small intestine was perfused (n = 6) and GLP-1 output from perfused mouse small intestine was investigated in response to vascular ghrelin administration in the presence and absence of a simultaneous luminal glucose stimulus. Ghrelin receptor expression was quantified in human (n = 11) and mouse L-cells (n = 3) by RNA sequencing and RT-qPCR, respectively. RESULTS: Ghrelin did not affect GLP-1 secretion in humans (area under the curve [AUC; 0-120 min]: ghrelin infusion = 1.37 ± 0.05 min × nmol vs. saline infusion = 1.40 ± 0.06 min × nmol [P = 0.63]), but induced peripheral insulin resistance. Likewise, ghrelin did not stimulate GLP-1 secretion from the perfused mouse small intestine model (mean outputs during baseline/ghrelin infusion = 19.3 ± 1.6/25.5 ± 2.0 fmol/min, n = 6, P = 0.16), whereas glucose-dependent insulinotropic polypeptide administration, used as a positive control, doubled GLP-1 secretion (P < 0.001). Intraluminal glucose increased GLP-1 secretion by 4-fold (P < 0.001), which was not potentiated by ghrelin. Finally, gene expression of the ghrelin receptor was undetectable in mouse L-cells and marginal in human L-cells. CONCLUSIONS: Ghrelin does not interact directly with the L-cell and does not directly affect GLP-1 secretion.

AB - CONTEXT: The gastrointestinal hormone ghrelin stimulates growth hormone secretion and appetite, but recent studies indicate that ghrelin also stimulates the secretion of the appetite-inhibiting and insulinotropic hormone glucagon-like peptide-1 (GLP-1). OBJECTIVE: To investigate the putative effect of ghrelin on GLP-1 secretion in vivo and in vitro. SUBJECTS AND METHODS: A randomized placebo-controlled crossover study was performed in eight hypopituitary subjects. Ghrelin or saline was infused intravenously (1 pmol/min × kg) after collection of baseline sample (0 min), and blood was subsequently collected at time 30, 60, 90, and 120 minutes. Mouse small intestine was perfused (n = 6) and GLP-1 output from perfused mouse small intestine was investigated in response to vascular ghrelin administration in the presence and absence of a simultaneous luminal glucose stimulus. Ghrelin receptor expression was quantified in human (n = 11) and mouse L-cells (n = 3) by RNA sequencing and RT-qPCR, respectively. RESULTS: Ghrelin did not affect GLP-1 secretion in humans (area under the curve [AUC; 0-120 min]: ghrelin infusion = 1.37 ± 0.05 min × nmol vs. saline infusion = 1.40 ± 0.06 min × nmol [P = 0.63]), but induced peripheral insulin resistance. Likewise, ghrelin did not stimulate GLP-1 secretion from the perfused mouse small intestine model (mean outputs during baseline/ghrelin infusion = 19.3 ± 1.6/25.5 ± 2.0 fmol/min, n = 6, P = 0.16), whereas glucose-dependent insulinotropic polypeptide administration, used as a positive control, doubled GLP-1 secretion (P < 0.001). Intraluminal glucose increased GLP-1 secretion by 4-fold (P < 0.001), which was not potentiated by ghrelin. Finally, gene expression of the ghrelin receptor was undetectable in mouse L-cells and marginal in human L-cells. CONCLUSIONS: Ghrelin does not interact directly with the L-cell and does not directly affect GLP-1 secretion.

KW - CELLS

KW - GLP-1 SECRETION

KW - GLUCOSE-ABSORPTION

KW - HORMONES

KW - INSULIN-SECRETION

KW - MEAL

KW - MECHANISMS

KW - RECEPTOR

KW - SURGERY

KW - WEIGHT

U2 - 10.1210/clinem/dgz046

DO - 10.1210/clinem/dgz046

M3 - Journal article

C2 - 31608930

VL - 105

SP - 266

EP - 275

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 1

M1 - dgz046

ER -