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Esben Meldgaard Høgh Quistgaard

Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms

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Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms. / Quistgaard, Esben M; Löw, Christian; Moberg, Per; Nordlund, Pär.

In: Journal of Structural Biology, Vol. 184, No. 2, 11.2013, p. 375-8.

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Quistgaard, Esben M ; Löw, Christian ; Moberg, Per ; Nordlund, Pär. / Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms. In: Journal of Structural Biology. 2013 ; Vol. 184, No. 2. pp. 375-8.

Bibtex

@article{5f69651569814b278208ed6acaf19e25,
title = "Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms",
abstract = "XylE is a major facilitator (MFS) xylose transporter, which is homologous to the mammalian glucose transporters (GLUT family). We have previously reported the structure of XylE in fully inward open and partially occluded inward open conformations in space groups P61 and C2, respectively. Here we present the crystallization of a third crystal form, P212121 (~4 {\AA} resolution), also representing an inward facing conformation, and analyze all three forms in terms of crystallization conditions and packing. The crystallization conditions were generally very similar with only slight changes needed to favor one form over another, e.g. the presence of lanthanide ions greatly favors C2 over P212121 under otherwise identical conditions. Cadmium was essential for crystallization of all three forms, which indeed all contain a Cd(2+) ion in a crystal packing interface, though surprisingly in different positions. Cadmium was also found to bind to XylE in solution. The diffraction data were highly anisotropic for all forms, reflecting a lack of ordered crystal contacts along one or two of the cell axes. The best diffracting directions thus consistently correlate with the presence of ordered contacts, most of which are metal-mediated. The data presented here highlight the utility of metal ions in membrane protein crystallization and suggest that metal site engineering may be a productive path towards obtaining additional crystal forms of XylE and other membrane proteins.",
keywords = "Cadmium, Crystallization, Crystallography, X-Ray, Escherichia coli, Escherichia coli Proteins, Lanthanoid Series Elements, Protein Binding, Protein Stability, Solutions, Symporters, Journal Article, Research Support, Non-U.S. Gov't",
author = "Quistgaard, {Esben M} and Christian L{\"o}w and Per Moberg and P{\"a}r Nordlund",
note = "Copyright {\circledC} 2013 Elsevier Inc. All rights reserved.",
year = "2013",
month = "11",
doi = "10.1016/j.jsb.2013.09.009",
language = "English",
volume = "184",
pages = "375--8",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms

AU - Quistgaard, Esben M

AU - Löw, Christian

AU - Moberg, Per

AU - Nordlund, Pär

N1 - Copyright © 2013 Elsevier Inc. All rights reserved.

PY - 2013/11

Y1 - 2013/11

N2 - XylE is a major facilitator (MFS) xylose transporter, which is homologous to the mammalian glucose transporters (GLUT family). We have previously reported the structure of XylE in fully inward open and partially occluded inward open conformations in space groups P61 and C2, respectively. Here we present the crystallization of a third crystal form, P212121 (~4 Å resolution), also representing an inward facing conformation, and analyze all three forms in terms of crystallization conditions and packing. The crystallization conditions were generally very similar with only slight changes needed to favor one form over another, e.g. the presence of lanthanide ions greatly favors C2 over P212121 under otherwise identical conditions. Cadmium was essential for crystallization of all three forms, which indeed all contain a Cd(2+) ion in a crystal packing interface, though surprisingly in different positions. Cadmium was also found to bind to XylE in solution. The diffraction data were highly anisotropic for all forms, reflecting a lack of ordered crystal contacts along one or two of the cell axes. The best diffracting directions thus consistently correlate with the presence of ordered contacts, most of which are metal-mediated. The data presented here highlight the utility of metal ions in membrane protein crystallization and suggest that metal site engineering may be a productive path towards obtaining additional crystal forms of XylE and other membrane proteins.

AB - XylE is a major facilitator (MFS) xylose transporter, which is homologous to the mammalian glucose transporters (GLUT family). We have previously reported the structure of XylE in fully inward open and partially occluded inward open conformations in space groups P61 and C2, respectively. Here we present the crystallization of a third crystal form, P212121 (~4 Å resolution), also representing an inward facing conformation, and analyze all three forms in terms of crystallization conditions and packing. The crystallization conditions were generally very similar with only slight changes needed to favor one form over another, e.g. the presence of lanthanide ions greatly favors C2 over P212121 under otherwise identical conditions. Cadmium was essential for crystallization of all three forms, which indeed all contain a Cd(2+) ion in a crystal packing interface, though surprisingly in different positions. Cadmium was also found to bind to XylE in solution. The diffraction data were highly anisotropic for all forms, reflecting a lack of ordered crystal contacts along one or two of the cell axes. The best diffracting directions thus consistently correlate with the presence of ordered contacts, most of which are metal-mediated. The data presented here highlight the utility of metal ions in membrane protein crystallization and suggest that metal site engineering may be a productive path towards obtaining additional crystal forms of XylE and other membrane proteins.

KW - Cadmium

KW - Crystallization

KW - Crystallography, X-Ray

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Lanthanoid Series Elements

KW - Protein Binding

KW - Protein Stability

KW - Solutions

KW - Symporters

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.jsb.2013.09.009

DO - 10.1016/j.jsb.2013.09.009

M3 - Journal article

C2 - 24060988

VL - 184

SP - 375

EP - 378

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

IS - 2

ER -