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Erik Jeppesen

Discovering hidden biodiversity: The use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystems

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Discovering hidden biodiversity: The use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystems. / Jo, Hyunbin; Ventura, Marc; Vidal, Nicolas; Gim, Jeong-Soo; Buchaca, Teresa; Barmuta, Leon ; Jeppesen, Erik; Joo, Gea-Jae.

2015.

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Jo, Hyunbin ; Ventura, Marc ; Vidal, Nicolas ; Gim, Jeong-Soo ; Buchaca, Teresa ; Barmuta, Leon ; Jeppesen, Erik ; Joo, Gea-Jae. / Discovering hidden biodiversity: The use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystems. 2015.

Bibtex

@techreport{ee7ce047c80d4e23989abe255e5720b3,
title = "Discovering hidden biodiversity: The use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystems",
abstract = "Ecological monitoring contributes to the understanding of complex ecosystem functions. The diets of fish reflect the surrounding environment and habitats and may, therefore, act as useful integrating indicators of environmental status. It is, however, often difficult to visually identify items in gut contents to species level due to digestion of soft-bodied prey beyond visual recognition, but new tools rendering this possible are now becoming available. We used a molecular approach to determine the species identities of consumed diet items of an introduced generalist feeder, brown trout (Salmo trutta), in 10 Tasmanian lakes and compared the results with those obtained from visual inspection of stomach contents. We obtained 44 unique taxa (OTUs) belonging to 5 phyla, including 7 classes, using the barcode of life approach from cytochrome oxidase I (COI). Compared with visual inspection, DNA analysis showed greater accuracy, yielding a 1.4-fold higher number of OTUs. Rarefaction curve analysis showed saturation of visually inspected taxa, while the curves from the DNA barcode did not saturate. The OTUs with the highest proportions of haplotypes were the families of terrestrial insects Formicidae, Chrysomelidae and Torbidae and the freshwater Chironomidae. The haplotype occurrence per lake was negatively correlated with lake depth and transparency. Nearly all haplotypes were only found in one fish gut from a single lake. Our results indicate that DNA barcoding of fish diets is a useful and complementary method for discovering hidden biodiversity. ",
author = "Hyunbin Jo and Marc Ventura and Nicolas Vidal and Jeong-Soo Gim and Teresa Buchaca and Leon Barmuta and Erik Jeppesen and Gea-Jae Joo",
year = "2015",
language = "English",
type = "WorkingPaper",

}

RIS

TY - UNPB

T1 - Discovering hidden biodiversity: The use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystems

AU - Jo, Hyunbin

AU - Ventura, Marc

AU - Vidal, Nicolas

AU - Gim, Jeong-Soo

AU - Buchaca, Teresa

AU - Barmuta, Leon

AU - Jeppesen, Erik

AU - Joo, Gea-Jae

PY - 2015

Y1 - 2015

N2 - Ecological monitoring contributes to the understanding of complex ecosystem functions. The diets of fish reflect the surrounding environment and habitats and may, therefore, act as useful integrating indicators of environmental status. It is, however, often difficult to visually identify items in gut contents to species level due to digestion of soft-bodied prey beyond visual recognition, but new tools rendering this possible are now becoming available. We used a molecular approach to determine the species identities of consumed diet items of an introduced generalist feeder, brown trout (Salmo trutta), in 10 Tasmanian lakes and compared the results with those obtained from visual inspection of stomach contents. We obtained 44 unique taxa (OTUs) belonging to 5 phyla, including 7 classes, using the barcode of life approach from cytochrome oxidase I (COI). Compared with visual inspection, DNA analysis showed greater accuracy, yielding a 1.4-fold higher number of OTUs. Rarefaction curve analysis showed saturation of visually inspected taxa, while the curves from the DNA barcode did not saturate. The OTUs with the highest proportions of haplotypes were the families of terrestrial insects Formicidae, Chrysomelidae and Torbidae and the freshwater Chironomidae. The haplotype occurrence per lake was negatively correlated with lake depth and transparency. Nearly all haplotypes were only found in one fish gut from a single lake. Our results indicate that DNA barcoding of fish diets is a useful and complementary method for discovering hidden biodiversity.

AB - Ecological monitoring contributes to the understanding of complex ecosystem functions. The diets of fish reflect the surrounding environment and habitats and may, therefore, act as useful integrating indicators of environmental status. It is, however, often difficult to visually identify items in gut contents to species level due to digestion of soft-bodied prey beyond visual recognition, but new tools rendering this possible are now becoming available. We used a molecular approach to determine the species identities of consumed diet items of an introduced generalist feeder, brown trout (Salmo trutta), in 10 Tasmanian lakes and compared the results with those obtained from visual inspection of stomach contents. We obtained 44 unique taxa (OTUs) belonging to 5 phyla, including 7 classes, using the barcode of life approach from cytochrome oxidase I (COI). Compared with visual inspection, DNA analysis showed greater accuracy, yielding a 1.4-fold higher number of OTUs. Rarefaction curve analysis showed saturation of visually inspected taxa, while the curves from the DNA barcode did not saturate. The OTUs with the highest proportions of haplotypes were the families of terrestrial insects Formicidae, Chrysomelidae and Torbidae and the freshwater Chironomidae. The haplotype occurrence per lake was negatively correlated with lake depth and transparency. Nearly all haplotypes were only found in one fish gut from a single lake. Our results indicate that DNA barcoding of fish diets is a useful and complementary method for discovering hidden biodiversity.

M3 - Working paper

BT - Discovering hidden biodiversity: The use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystems

ER -