Erik Ernst

Evaluation of male fertility potential by Toluidine Blue test for sperm chromatin structure assessment

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • I Tsarev, Denmark
  • M Bungum, Denmark
  • A Giwercman, Denmark
  • J Erenpreisa, Denmark
  • T Ebessen, Denmark
  • E Ernst
  • J Erenpreiss, Denmark
  • Department of Anatomy
BACKGROUND: We have previously suggested that the Toluidine Blue (TB) test can be used for sperm chromatin structure assessment. In this study, we wished to evaluate the clinical applicability of the TB test in assessing male fertility potential using well-defined groups of fertile and infertile men. METHODS: Sixty-three fertile and 79 infertile men were tested. Infertility thresholds for the proportion of sperm with abnormal [TB dark cells (TBDCs)] and normal [TB light cells (TBLCs)] chromatin structure were set by the ROC curve analysis. RESULTS: Thresholds of 45% TBDC and 20% TBLC were highly predictive for infertility (specificity of the test: 92 and 90%, respectively), but they were poor predictors of the fertility (sensitivity of the test: 42 and 32%, respectively). Odds ratio for infertility was 7.5 [95% confidence interval (CI): 2.7-20.8] when the 45% TBDC threshold was used and 4.4 (95% CI: 1.7-11.6) when the 20% TBLC threshold was used. CONCLUSIONS: The TB test can be suggested for clinical use as a complementary test for standard semen analysis to diagnose male infertility.
Original languageEnglish
JournalHuman Reproduction
Pages (from-to)1569-74
Number of pages5
Publication statusPublished - 2009

    Research areas

  • Chromatin, Female, Fertility, Humans, Infertility, Male, Male, Odds Ratio, Pregnancy, ROC Curve, Semen, Semen Analysis, Sensitivity and Specificity, Sperm Count, Spermatozoa, Tolonium Chloride

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