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Erik Baatrup

Autometallography: tissue metals demonstrated by a silver enhancement kit.

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  • Department of Anatomy
  • Interdisciplinary Nanoscience Center
  • Department of Biological Sciences, Zoophysiology
In biological tissue, minute accumulations of gold, silver, mercury and zinc can be visualized by a technique whereby metallic silver is precipitated on tiny accumulations of the two noble metals, or on selenites or sulphides of all four metals. In the present study a silver enhancement kit, primarily intended for the amplification of colloidal gold particles, has been used to demonstrate these catalytic tissue metals. Sections from animals exposed intravitally to aurothiomalatate, silver lactate, mercury chloride, sodium selenite or perfused with sodium sulphide were subjected to a commercial silver enhancement kit (IntenSE, Janssen Pharmaceutica). It was found that the kit performs adequately to the silver lactate gum arabic developer and to the photographic emulsion technique. The kit can be used as a silver enhancement medium for the demonstration of zinc by the Neo-Timm and selenium methods and for demonstration of gold, silver, and mercury in tissues from animals intravitally exposed to these metals. It can also be used for counterstaining silver treated osmium fixed tissues embedded in plastic.
Udgivelsesdato: 1987-null
Original languageEnglish
JournalHistochemistry
Volume86
Issue5
Pages (from-to)465-9
Number of pages4
ISSN0301-5564
Publication statusPublished - 1987

    Research areas

  • Animals, Brain Chemistry, Gold, Histocytochemistry, Kidney, Male, Mercury, Metals, Mice, Rats, Rats, Inbred Strains, Silver, Zinc

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