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Ebbe Toftgaard Poulsen

Assistant Professor

Personal information

Name: Ebbe Toftgaard Poulsen
Date of birth: 02-09-1982
Contact information:E-mail: etp@mbg.au.dk

Positions

01/02/2018–presentAssistant professor, Protein chemistry and proteomics, Department of Molecular Biology and Genetics, Aarhus University, Denmark
01/02/2013 - 31/01/2018Postdoc, Protein chemistry and proteomics, Department of Molecular Biology and Genetics, Aarhus University, Denmark

Education

01/08/2009–31/01/2013Ph.D., Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark
01/09/2006-31/01/2011M.Sc. Molecular Biology (Part A exam), Department of Molecular Biology, Aarhus University, Denmark
01/08/2003-31/06/2006 B.Sc. Molecular Biology, Department of Molecular Biology, Aarhus University, Denmark

International experience

15/07/2010-15/09/2010 Research stay as part of the PhD, Duke Eye Center, Duke University, North Carolina, USA
15/01/2007-15/09/2007 Research project, Murdoch Children’s Research Institute, Royal Children’s Hospital, Melbourne, Australia

Paternity leave

2019Three months paternity leave
2015Three months paternity leave

Scientific research area

My main area of focus is protein and proteome characterization of extracellular matrix proteins, in particular the corneal extracellular matrix. Additionally, I have worked with amyloid-related projects, including lattice corneal dystrophy, functional amyloid from bacteria, and the amyloid precursor protein in relation to dementia. In my research, I use both recombinant protein expression and purification, or purify protein from its natural source such as human tissue and plasma. I am trained in various classical protein chemistry methods including 1D and 2D gel electrophoresis, Western blotting, ELISA, liquid chromatography (reverse phase, gel filtration, ion exchange etc.), and Edman degradation. Over the years, I have specialized within advanced mass spectrometry (Sciex and Thermo instruments) allowing me not only to perform proteomics studies (label-free, iTRAQ/TMT, SRM, PRM, SWATH), but also to use mass spectrometry for protein characterization (chemical cross-linking, chemical foot-printing, bead pull-down experiments). Lastly, I am trained in laser capture microdissection from tissue sections, a technique very applicable with mass spectrometry analyses.

Managerial positions

2017 – 2022Project leader, The window of the eye, Cornea dystrophy, maintaining transparency, funded by the VELUX Foundation

Bibliometrics

2010 – 2020Author of 27 peer-reviewed articles, nine as fist author (PubMed). H-index: 11, citations 328, average citation 13.12 (Web of Science)

External funding

2013 – 2020PI: 320.000 DKK, Co-PI: 10.3 mio. DKK, Co-author: 10.9 mio. DKK

Supervision

2013 – presentCo-supervisor of one bachelor student
2013 – presentCo-supervisor of two master students
2013 – presentCo-supervisor of five molecular biology projects
2014 – presentCo-supervisor of four PhD students
2015 – presentCo-supervisor of two visiting researchers

Teaching experience (Aarhus University)

2017-2019Experimental Biochemistry
2015-2016Experimental Nano-Exercise
2011-2017Applied Proteomics
2009-2016Advanced Biochemistry
2008Introduction to Biochemistry

Invited speaker

2018ISER Scientific Congress, Belfast, Northern Ireland
2014Meeting at the Danish Society of Mass Spectrometry

Evaluation

- Thesis evaluation at Bachelor level, Master level, and Ph.D level
- Examination at Bachelor and Master levels
- Peer-review of manuscripts and grant-proposals within the fields of eye research and proteomics