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Daniel Otzen

The role of protonation in protein fibrillation

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The role of protonation in protein fibrillation. / Jeppesen, Martin D; Westh, Peter; Otzen, Daniel E.

In: FEBS Letters, Vol. 584, No. 4, 19.02.2010, p. 780-4.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Jeppesen, MD, Westh, P & Otzen, DE 2010, 'The role of protonation in protein fibrillation', FEBS Letters, vol. 584, no. 4, pp. 780-4. https://doi.org/10.1016/j.febslet.2010.01.002

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Jeppesen, Martin D ; Westh, Peter ; Otzen, Daniel E. / The role of protonation in protein fibrillation. In: FEBS Letters. 2010 ; Vol. 584, No. 4. pp. 780-4.

Bibtex

@article{0ed4d897994641c5bd53a41aae325f2c,
title = "The role of protonation in protein fibrillation",
abstract = "Many proteins fibrillate at low pH despite a high population of charged side chains. Therefore exchange of protons between the fibrillating peptide and its surroundings may play an important role in fibrillation. Here, we use isothermal titration calorimetry to measure exchange of protons between buffer and the peptide hormone glucagon during fibrillation. Glucagon absorbs or releases protons to an extent which allows it to attain a net charge of zero in the fibrillar state, both at acidic and basic pH. Similar results are obtained for lysozyme. This suggests that side chain pK(a) values change dramatically in the fibrillar state.",
keywords = "Buffers, Calorimetry, Circular Dichroism, Glucagon, Hydrogen-Ion Concentration, Kinetics, Microscopy, Atomic Force, Models, Chemical, Muramidase, Protein Folding, Proteins, Protons, Thermodynamics, Titrimetry",
author = "Jeppesen, {Martin D} and Peter Westh and Otzen, {Daniel E}",
note = "Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",
year = "2010",
month = feb,
day = "19",
doi = "10.1016/j.febslet.2010.01.002",
language = "English",
volume = "584",
pages = "780--4",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - The role of protonation in protein fibrillation

AU - Jeppesen, Martin D

AU - Westh, Peter

AU - Otzen, Daniel E

N1 - Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

PY - 2010/2/19

Y1 - 2010/2/19

N2 - Many proteins fibrillate at low pH despite a high population of charged side chains. Therefore exchange of protons between the fibrillating peptide and its surroundings may play an important role in fibrillation. Here, we use isothermal titration calorimetry to measure exchange of protons between buffer and the peptide hormone glucagon during fibrillation. Glucagon absorbs or releases protons to an extent which allows it to attain a net charge of zero in the fibrillar state, both at acidic and basic pH. Similar results are obtained for lysozyme. This suggests that side chain pK(a) values change dramatically in the fibrillar state.

AB - Many proteins fibrillate at low pH despite a high population of charged side chains. Therefore exchange of protons between the fibrillating peptide and its surroundings may play an important role in fibrillation. Here, we use isothermal titration calorimetry to measure exchange of protons between buffer and the peptide hormone glucagon during fibrillation. Glucagon absorbs or releases protons to an extent which allows it to attain a net charge of zero in the fibrillar state, both at acidic and basic pH. Similar results are obtained for lysozyme. This suggests that side chain pK(a) values change dramatically in the fibrillar state.

KW - Buffers

KW - Calorimetry

KW - Circular Dichroism

KW - Glucagon

KW - Hydrogen-Ion Concentration

KW - Kinetics

KW - Microscopy, Atomic Force

KW - Models, Chemical

KW - Muramidase

KW - Protein Folding

KW - Proteins

KW - Protons

KW - Thermodynamics

KW - Titrimetry

U2 - 10.1016/j.febslet.2010.01.002

DO - 10.1016/j.febslet.2010.01.002

M3 - Journal article

C2 - 20067793

VL - 584

SP - 780

EP - 784

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 4

ER -