Aarhus University Seal / Aarhus Universitets segl

Daniel Otzen

The effect of osmolytes on protein fibrillation

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Documents

DOI

  • Francesca Macchi, Denmark
  • Maike Eisenkolb, Hochschule Biberach, Pharmaceutical Biotechnology, Germany
  • Hans Kiefer, Hochschule Biberach, Pharmaceutical Biotechnology, Germany
  • Daniel Otzen
Osmolytes are small molecules that are exploited by cells as a protective system against stress conditions. They favour compact protein states which makes them stabilize globular proteins in vitro and promote folding. Conversely, this preference for compact states promotes aggregation of unstructured proteins. Here we combine a brief review of the effect of osmolytes on protein fibrillation with a report of the effect of osmolytes on the unstructured peptide hormone glucagon. Our results show that osmolytes either accelerate the fibrillation kinetics or leave them unaffected, with the exception of the osmolyte taurine. Furthermore, the osmolytes that affected the shape of the fibrillation time profile led to fibrils with different structure as revealed by CD. The structural changes induced by Pro, Ser and choline-O-sulfate could be due to specific osmolytes binding to the peptides, stabilizing an otherwise labile fibrillation intermediate.
Original languageEnglish
JournalInternational Journal of Molecular Sciences (Online)
Volume13
Issue3
Pages (from-to)3801-3819
Number of pages19
ISSN1661-6596
DOIs
Publication statusPublished - 21 Mar 2012

See relations at Aarhus University Citationformats

Download statistics

No data available

ID: 48904491