TY - JOUR

T1 - Nanosilver Mitigates Biofilm Formation via FapC Amyloidosis Inhibition

AU - Huma, Zil-E

AU - Javed, Ibrahim

AU - Zhang, Zhenzhen

AU - Bilal, Hajira

AU - Sun, Yunxiang

AU - Hussain, Syed Zajif

AU - Davis, Thomas P

AU - Otzen, Daniel E

AU - Landersdorfer, Cornelia B

AU - Ding, Feng

AU - Hussain, Irshad

AU - Ke, Pu Chun

N1 - © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2020/5

Y1 - 2020/5

N2 - Multidrug resistance of bacteria is a major challenge due to the wide-spread use of antibiotics. While a range of strategies have been developed in recent years, suppression of bacterial activity and virulence via their network of extracellular amyloid has rarely been explored, especially with nanomaterials. Here, silver nanoparticles and nanoclusters (AgNPs and AgNCs) capped with cationic branched polyethylenimine polymer are synthesized, and their antimicrobial potentials are determined at concentrations safe to mammalian cells. Compared with the ultrasmall AgNCs, AgNPs entail stronger binding to suppress the fibrillization of FapC, a major protein constituent of the extracellular amyloid matrix of Pseudomonas aeruginosa. Both types of nanoparticles exhibit concentration-dependent antibiofilm and antimicrobial properties against P. aeruginosa. At concentrations of 1 × 10-6 m or below, both the bactericidal activity of AgNCs and the antibiofilm capacity of AgNPs are associated with their structure-mediated bio-nano interactions but not ion release. For AgNPs, specifically, their antibiofilm potency correlates with their capacity of FapC fibrillization inhibition, but not with their bactericidal activity. This study demonstrates the antimicrobial potential of safe nanotechnology through the novel route of amyloidosis inhibition.

AB - Multidrug resistance of bacteria is a major challenge due to the wide-spread use of antibiotics. While a range of strategies have been developed in recent years, suppression of bacterial activity and virulence via their network of extracellular amyloid has rarely been explored, especially with nanomaterials. Here, silver nanoparticles and nanoclusters (AgNPs and AgNCs) capped with cationic branched polyethylenimine polymer are synthesized, and their antimicrobial potentials are determined at concentrations safe to mammalian cells. Compared with the ultrasmall AgNCs, AgNPs entail stronger binding to suppress the fibrillization of FapC, a major protein constituent of the extracellular amyloid matrix of Pseudomonas aeruginosa. Both types of nanoparticles exhibit concentration-dependent antibiofilm and antimicrobial properties against P. aeruginosa. At concentrations of 1 × 10-6 m or below, both the bactericidal activity of AgNCs and the antibiofilm capacity of AgNPs are associated with their structure-mediated bio-nano interactions but not ion release. For AgNPs, specifically, their antibiofilm potency correlates with their capacity of FapC fibrillization inhibition, but not with their bactericidal activity. This study demonstrates the antimicrobial potential of safe nanotechnology through the novel route of amyloidosis inhibition.

KW - FapC

KW - amyloids

KW - antibiofilms

KW - silver nanoclusters

KW - silver nanoparticles

KW - FIBRIL FORMATION

KW - GOLD NANOPARTICLES

KW - BACTERIA

KW - DISCRETE MOLECULAR-DYNAMICS

KW - SILVER NANOPARTICLES

KW - NANOCLUSTERS

KW - OXIDE NANOPARTICLES

KW - ANTIBACTERIAL ACTIVITY

KW - PSEUDOMONAS-AERUGINOSA

KW - EFFICIENT

U2 - 10.1002/smll.201906674

DO - 10.1002/smll.201906674

M3 - Journal article

C2 - 31984626

VL - 16

JO - Small

JF - Small

SN - 1613-6810

IS - 21

M1 - 1906674

ER -