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Daniel Otzen

MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperReviewResearchpeer-review

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MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments. / Martins, Pedro M; Navarro, Susanna; Silva, Alexandra et al.

In: Frontiers in Molecular Neuroscience, Vol. 13, 582488, 27.11.2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperReviewResearchpeer-review

Harvard

Martins, PM, Navarro, S, Silva, A, Pinto, MF, Sárkány, Z, Figueiredo, F, Pereira, PJB, Pinheiro, F, Bednarikova, Z, Burdukiewicz, M, Galzitskaya, OV, Gazova, Z, Gomes, CM, Pastore, A, Serpell, LC, Skrabana, R, Smirnovas, V, Ziaunys, M, Otzen, DE, Ventura, S & Macedo-Ribeiro, S 2020, 'MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments', Frontiers in Molecular Neuroscience, vol. 13, 582488. https://doi.org/10.3389/fnmol.2020.582488

APA

Martins, P. M., Navarro, S., Silva, A., Pinto, M. F., Sárkány, Z., Figueiredo, F., Pereira, P. J. B., Pinheiro, F., Bednarikova, Z., Burdukiewicz, M., Galzitskaya, O. V., Gazova, Z., Gomes, C. M., Pastore, A., Serpell, L. C., Skrabana, R., Smirnovas, V., Ziaunys, M., Otzen, D. E., ... Macedo-Ribeiro, S. (2020). MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments. Frontiers in Molecular Neuroscience, 13, [582488]. https://doi.org/10.3389/fnmol.2020.582488

CBE

Martins PM, Navarro S, Silva A, Pinto MF, Sárkány Z, Figueiredo F, Pereira PJB, Pinheiro F, Bednarikova Z, Burdukiewicz M, et al. 2020. MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments. Frontiers in Molecular Neuroscience. 13:Article 582488. https://doi.org/10.3389/fnmol.2020.582488

MLA

Vancouver

Martins PM, Navarro S, Silva A, Pinto MF, Sárkány Z, Figueiredo F et al. MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments. Frontiers in Molecular Neuroscience. 2020 Nov 27;13:582488. doi: 10.3389/fnmol.2020.582488

Author

Martins, Pedro M ; Navarro, Susanna ; Silva, Alexandra et al. / MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments. In: Frontiers in Molecular Neuroscience. 2020 ; Vol. 13.

Bibtex

@article{3e8b74a7689447e1ac11fd7f7045cdc8,
title = "MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments",
abstract = "Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.",
author = "Martins, {Pedro M} and Susanna Navarro and Alexandra Silva and Pinto, {Maria F} and Zsuzsa S{\'a}rk{\'a}ny and Francisco Figueiredo and Pereira, {Pedro Jos{\'e} Barbosa} and Francisca Pinheiro and Zuzana Bednarikova and Micha{\l} Burdukiewicz and Galzitskaya, {Oxana V} and Zuzana Gazova and Gomes, {Cl{\'a}udio M} and Annalisa Pastore and Serpell, {Louise C} and Rostislav Skrabana and Vytautas Smirnovas and Mantas Ziaunys and Otzen, {Daniel E} and Salvador Ventura and Sandra Macedo-Ribeiro",
note = "Copyright {\textcopyright} 2020 Martins, Navarro, Silva, Pinto, S{\'a}rk{\'a}ny, Figueiredo, Pereira, Pinheiro, Bednarikova, Burdukiewicz, Galzitskaya, Gazova, Gomes, Pastore, Serpell, Skrabana, Smirnovas, Ziaunys, Otzen, Ventura and Macedo-Ribeiro.",
year = "2020",
month = nov,
day = "27",
doi = "10.3389/fnmol.2020.582488",
language = "English",
volume = "13",
journal = "Frontiers in Molecular Neuroscience",
issn = "1662-5099",
publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments

AU - Martins, Pedro M

AU - Navarro, Susanna

AU - Silva, Alexandra

AU - Pinto, Maria F

AU - Sárkány, Zsuzsa

AU - Figueiredo, Francisco

AU - Pereira, Pedro José Barbosa

AU - Pinheiro, Francisca

AU - Bednarikova, Zuzana

AU - Burdukiewicz, Michał

AU - Galzitskaya, Oxana V

AU - Gazova, Zuzana

AU - Gomes, Cláudio M

AU - Pastore, Annalisa

AU - Serpell, Louise C

AU - Skrabana, Rostislav

AU - Smirnovas, Vytautas

AU - Ziaunys, Mantas

AU - Otzen, Daniel E

AU - Ventura, Salvador

AU - Macedo-Ribeiro, Sandra

N1 - Copyright © 2020 Martins, Navarro, Silva, Pinto, Sárkány, Figueiredo, Pereira, Pinheiro, Bednarikova, Burdukiewicz, Galzitskaya, Gazova, Gomes, Pastore, Serpell, Skrabana, Smirnovas, Ziaunys, Otzen, Ventura and Macedo-Ribeiro.

PY - 2020/11/27

Y1 - 2020/11/27

N2 - Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.

AB - Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.

U2 - 10.3389/fnmol.2020.582488

DO - 10.3389/fnmol.2020.582488

M3 - Review

C2 - 33328883

VL - 13

JO - Frontiers in Molecular Neuroscience

JF - Frontiers in Molecular Neuroscience

SN - 1662-5099

M1 - 582488

ER -