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Daniel Otzen

Dual hydrolytic role for Pb(II) ions

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Standard

Dual hydrolytic role for Pb(II) ions. / Otzen, D. E.; Barciszewski, J.; Clark, B. F.C.

In: Biochimie, Vol. 76, No. 1, 01.01.1994, p. 15-21.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Otzen, DE, Barciszewski, J & Clark, BFC 1994, 'Dual hydrolytic role for Pb(II) ions', Biochimie, vol. 76, no. 1, pp. 15-21. https://doi.org/10.1016/0300-9084(94)90058-2

APA

Otzen, D. E., Barciszewski, J., & Clark, B. F. C. (1994). Dual hydrolytic role for Pb(II) ions. Biochimie, 76(1), 15-21. https://doi.org/10.1016/0300-9084(94)90058-2

CBE

Otzen DE, Barciszewski J, Clark BFC. 1994. Dual hydrolytic role for Pb(II) ions. Biochimie. 76(1):15-21. https://doi.org/10.1016/0300-9084(94)90058-2

MLA

Otzen, D. E., J. Barciszewski and B. F.C. Clark. "Dual hydrolytic role for Pb(II) ions". Biochimie. 1994, 76(1). 15-21. https://doi.org/10.1016/0300-9084(94)90058-2

Vancouver

Otzen DE, Barciszewski J, Clark BFC. Dual hydrolytic role for Pb(II) ions. Biochimie. 1994 Jan 1;76(1):15-21. https://doi.org/10.1016/0300-9084(94)90058-2

Author

Otzen, D. E. ; Barciszewski, J. ; Clark, B. F.C. / Dual hydrolytic role for Pb(II) ions. In: Biochimie. 1994 ; Vol. 76, No. 1. pp. 15-21.

Bibtex

@article{55febef945984a4da08eae5dd5c6989e,
title = "Dual hydrolytic role for Pb(II) ions",
abstract = "RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3′-end of transfer RNA. Here we report that in aminoacylated transfer RNA, Pb2+ ions cleave internucleotide bonds in the 3′-end of tRNA and also cleaves the bond between tRNA and its amino-acid, attached at the 3′-end via an ester bond to the terminal ribose in aminoacyl tRNA. The two reactions proceed at different rates. The rate of deacylation is significantly faster than the rate of cleavage of phosphodiester bonds, with a pH-optimum of 7. This dual hydrolytic role is not seen for other metal ions examined, namely Zn(II), Cd(II) and Mn(II). The rate of the two kinds of hydrolyses by Pb2+ ions is compared with that of other metal-ions. The mechanism of cleavage is investigated further by modification of the 3′-end of tRNA.",
keywords = "aminoacylation, ester-bond hydrolysis, lead ions, metal ions, transfer-RNA",
author = "Otzen, {D. E.} and J. Barciszewski and Clark, {B. F.C.}",
year = "1994",
month = jan,
day = "1",
doi = "10.1016/0300-9084(94)90058-2",
language = "English",
volume = "76",
pages = "15--21",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier Masson",
number = "1",

}

RIS

TY - JOUR

T1 - Dual hydrolytic role for Pb(II) ions

AU - Otzen, D. E.

AU - Barciszewski, J.

AU - Clark, B. F.C.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3′-end of transfer RNA. Here we report that in aminoacylated transfer RNA, Pb2+ ions cleave internucleotide bonds in the 3′-end of tRNA and also cleaves the bond between tRNA and its amino-acid, attached at the 3′-end via an ester bond to the terminal ribose in aminoacyl tRNA. The two reactions proceed at different rates. The rate of deacylation is significantly faster than the rate of cleavage of phosphodiester bonds, with a pH-optimum of 7. This dual hydrolytic role is not seen for other metal ions examined, namely Zn(II), Cd(II) and Mn(II). The rate of the two kinds of hydrolyses by Pb2+ ions is compared with that of other metal-ions. The mechanism of cleavage is investigated further by modification of the 3′-end of tRNA.

AB - RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3′-end of transfer RNA. Here we report that in aminoacylated transfer RNA, Pb2+ ions cleave internucleotide bonds in the 3′-end of tRNA and also cleaves the bond between tRNA and its amino-acid, attached at the 3′-end via an ester bond to the terminal ribose in aminoacyl tRNA. The two reactions proceed at different rates. The rate of deacylation is significantly faster than the rate of cleavage of phosphodiester bonds, with a pH-optimum of 7. This dual hydrolytic role is not seen for other metal ions examined, namely Zn(II), Cd(II) and Mn(II). The rate of the two kinds of hydrolyses by Pb2+ ions is compared with that of other metal-ions. The mechanism of cleavage is investigated further by modification of the 3′-end of tRNA.

KW - aminoacylation

KW - ester-bond hydrolysis

KW - lead ions

KW - metal ions

KW - transfer-RNA

UR - http://www.scopus.com/inward/record.url?scp=0028198299&partnerID=8YFLogxK

U2 - 10.1016/0300-9084(94)90058-2

DO - 10.1016/0300-9084(94)90058-2

M3 - Journal article

C2 - 8031900

AN - SCOPUS:0028198299

VL - 76

SP - 15

EP - 21

JO - Biochimie

JF - Biochimie

SN - 0300-9084

IS - 1

ER -