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Daniel Otzen

Professor

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Daniel Otzen
Professor, Ph.D.
Interdisciplinary Nanoscience Center
Department of Molecular Biology and Genetics
Gustav Wieds Vej 14
8000 Aarhus C
Denmark
E-mail: dao@inano.au.dk
Web: www.proteins.dk
Phone: 87155441
Mobile: 20725238

CV

Born: 2. January 1969 in Sønderborg, Denmark
Private Address: Minthøjvej 1, DK – 8210 Aarhus V. Tel. +45 20 72 52 38.
Current position: rofessor of nanobiotechnology.
Place of Work: iNANO; Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus C.
Email: dao@inano.au.dk; web: http://www.proteins.dk
Education:
1992: M.Sc. (Chemistry and Biotechnology), Aarhus University
1995: Ph.D. (protein biophysics), Aarhus University and Cambridge University.
Appointments
1995-1997 Research Chemist, Novo Nordisk A/S
1997-2000 Post-doctoral fellow at Lund University
2000-2004 Associate Professor, Department of Life Sciences, Aalborg University.
2004-2007 Professor, Department of Life Sciences, Aalborg University
2007- Professor, iNANO, Aarhus University.
2006-2011 Adjunct Professor, Faculty of Pharmaceutical Sciences, Copenhagen
University
2007-2012 Adjunct Professor, Faculty of Engineering, Science and Medicine, Aalborg
University
Honours
2010: Member, Royal Danish Society of Science and Letters
2009: The Research Prize of the Alzheimer Research Foundation
2006: Carlsberg Biotechnology Prize
2003 : Silver Medal, Royal Danish Society of Sciences and Letters.
2003: EMBO Young Investigator
Leadership
2009- Leader, Lundbeck network for Bacterial Amyloid
2006-2011 Consortium Leader, Innovation Consortium CureND
2002-2007 Director of Doctoral School of Biotechnology, Aalborg University.
Supervision
Have completed supervision of 15 Ph.D. students.
Scientific papers
1993- >180 articles in international peer-reviewed journals
These include: 5 in PNAS, 2 in Molecular Microbiology, 7 in J. Biol. Chem., 22 in J. Mol. Biol., 19 in Biochemistry and 11 reviews.
> 4000 citations, H-factor 35.
Scholarly activity
2009- Editor, Biochimica et Biophysica Acta
2010- Editor, Biophysical Chemistry
Seminars > 35 seminars at international conferences and research institutes since 2000
Competences and research interests
• Protein-surfactant interactions
• Fibrillation and aggregation of proteins.
• Biophysics of membrane proteins, including stability and mechanism of insertion, folding and association in lipid bilayers and detergent micelles.
• Structure and mechanisms of antimicrobial peptides.
This is followed using optical spectroscopy, mechano-acoustic instrumentation, calorimetry and imaging equipment in combination with rapid reaction apparatus, protein engineering and mechanistic interpretation of the data.


Publications 1993-

Drug repurposing screens identify compounds that inhibit α-synuclein oligomers' membrane disruption and block antibody interactions

Somavarapu, A. K., Kleijwegt, G., Nagaraj, M., Alam, P., Nielsen, J. & Otzen, D. E., Mar 2023, In: Chemical Science. 14, 11, p. 3030-3047 18 p.

The anti-platelet drug ticlopidine inhibits FapC fibrillation and biofilm production: Highlighting its antibiotic activity

Pirhaghi, M., Najarzadeh, Z., Moosavi-Movahedi, F., Shafizadeh, M., Mamashli, F., Atarod, D., Ghasemi, A., Morshedi, D., Meratan, A. A., Otzen, D. E. & Saboury, A. A., Feb 2023, In: B B A - Proteins and Proteomics. 1871, 2, p. 140883 140883.

The impact of α-synuclein aggregates on blood-brain barrier integrity in the presence of neurovascular unit cells

Hourfar, H., Aliakbari, F., Aqdam, S. R., Nayeri, Z., Bardania, H., Otzen, D. E. & Morshedi, D., Feb 2023, In: International Journal of Biological Macromolecules. 229, p. 305-320 16 p.

FapA is an Intrinsically Disordered Chaperone for Pseudomonas Functional Amyloid FapC

Rasmussen, H. Ø., Kumar, A., Shin, B., Stylianou, F., Sewell, L., Xu, Y., Otzen, D. E., Pedersen, J. S. & Matthews, S. J., Jan 2023, In: Journal of Molecular Biology. 435, 2, 18 p., 167878.

Inhibitory effects of fluorinated benzenesulfonamides on insulin fibrillation

Alijanvand, S. H., Ladefoged, L. K., Zubrienė, A., Sakalauskas, A., Christiansen, G., Dudutiene, V., Schiøtt, B., Matulis, D., Smirnovas, V. & Otzen, D. E., 2023, In: International Journal of Biological Macromolecules. 227, p. 590-600 11 p.

A Semester-Long Learning Path Teaching Computational Skills via Molecular Graphics in PyMOL

Kjærgaard, M., Rasmussen, L. S., Vinther, J. N., Andersen, K. R., Andersen, E. S., Lorentzen, E., Thirup, S. S., Otzen, D. & Brodersen, D. E., Dec 2022, In: The Biophysicist. 3, 2, p. 106–114 9 p.

A penetratin-derived peptide reduces the membrane permeabilization and cell toxicity of α-synuclein oligomers

Pirhaghi, M., Frank, S. A., Alam, P., Nielsen, J., Sereikaite, V., Gupta, A., Strømgaard, K., Andreasen, M., Sharma, D., Saboury, A. A. & Otzen, D. E., Dec 2022, In: Journal of Biological Chemistry. 298, 12, 102688.

Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells

He, J., Steffen, J. H., Thulstrup, P. W., Pedersen, J. N., Sauerland, M. B., Otzen, D. E., Hawkins, C. L., Gourdon, P., Davies, M. J. & Hägglund, P., Dec 2022, In: Scientific Reports. 12, 1, 15 p., 22051.

EGCG has Dual and Opposing Effects on the N-terminal Region of Self-associating α-synuclein Oligomers

Grønnemose, A. L., Østerlund, E. C., Otzen, D. E. & Jørgensen, T. J. D., Dec 2022, In: Journal of Molecular Biology. 434, 23, 167855.

Functional amyloid in a lipid-like environment: a merry dance of many steps

Otzen, D. E., Dec 2022, In: Essays in Biochemistry. 66, 7

Molecular-level insights into the surface-induced assembly of Functional Bacterial Amyloid

Sønderby, T. V., Zou, Y., Wang, P., Wang, C. & Otzen, D. E., Sept 2022, In: Biophysical Journal. 121, 18, p. 3422-3434 13 p.

Uncovering the universality of self-replication in protein aggregation and its link to disease

Meisl, G., Xu, C. K., Taylor, J. D., Michaels, T. C. T., Levin, A., Otzen, D., Klenerman, D., Matthews, S., Linse, S., Andreasen, M. & Knowles, T. P. J., Aug 2022, In: Science Advances. 8, 32, eabn6831.

Structural Basis for Dityrosine-Mediated Inhibition of α-Synuclein Fibrillization

Sahin, C., Østerlund, E. C., Österlund, N., Costeira-Paulo, J., Pedersen, J. N., Christiansen, G., Nielsen, J., Grønnemose, A. L., Amstrup, S. K., Tiwari, M. K., Rao, R. S. P., Bjerrum, M. J., Ilag, L. L., Davies, M. J., Marklund, E. G., Pedersen, J. S., Landreh, M., Møller, I. M., Jørgensen, T. J. D. & Otzen, D. E., Jul 2022, In: Journal of the American Chemical Society. 144, 27, p. 11949-11954 6 p.

Structural variations between small alarmone hydrolase dimers support different modes of regulation of the stringent response

Bisiak, F., Chrenková, A., Zhang, S. D., Pedersen, J. N., Otzen, D. E., Zhang, Y. E. & Brodersen, D. E., Jul 2022, In: Journal of Biological Chemistry. 298, 7, 102142.

The optimal docking strength for reversibly tethered kinases

Dyla, M., González Foutel, N. S., Otzen, D. E. & Kjaergaard, M., 21 Jun 2022, In: Proceedings of the National Academy of Sciences. 119, 25, e2203098119.

Glycation modulates alpha-synuclein fibrillization kinetics: a sweet spot for inhibition

Farzadfard, A., König, A., Petersen, S. V., Nielsen, J., Vasili, E., Dominguez-Meijide, A., Buell, A. K., Outeiro, T. F. & Otzen, D. E., May 2022, In: The Journal of Biological Chemistry. 298, 5, 14 p., 101848.

The changing face of SDS denaturation: Complexes of Thermomyces lanuginosus lipase with SDS at pH 4.0, 6.0 and 8.0

Rasmussen, H. Ø., Wollenberg, D. T. W., Wang, H., Andersen, K. K., Oliveira, C. L. P., Jørgensen, C. I., Jørgensen, T. J. D., Otzen, D. E. & Pedersen, J. S., May 2022, In: Journal of Colloid and Interface Science. 614, p. 214-232 19 p.

Low dose DMSO treatment induces oligomerization and accelerates aggregation of α-synuclein

Reimer, L., Haikal, C., Gram, H., Theologidis, V., Kovacs, G., Ruesink, H., Baun, A., Nielsen, J., Otzen, D. E., Li, J-Y. & Jensen, P. H., Mar 2022, In: Scientific Reports. 12, 3737.

Bidirectional protein-protein interactions control liquid-liquid phase separation of PSD-95 and its interaction partners

Christensen, N. R., Pedersen, C. P., Sereikaite, V., Pedersen, J. N., Vistrup-Parry, M., Sørensen, A. T., Otzen, D., Teilum, K., Madsen, K. L. & Strømgaard, K., Feb 2022, In: iScience. 25, 2, 26 p., 103808.

Induction, inhibition, and incorporation: Different roles for anionic and zwitterionic lysolipids in the fibrillation of the functional amyloid FapC

Rasmussen, H. Ø., Otzen, D. E. & Pedersen, J. S., Feb 2022, In: The Journal of Biological Chemistry. 298, 2, 50 p., 101569.

The C-terminal tail of α-synuclein protects against aggregate replication but is critical for oligomerization

Farzadfard, A., Pedersen, J. N., Meisl, G., Somavarapu, A. K., Alam, P., Goksøyr, L., Nielsen, M. A., Sander, A. F., Knowles, T. P. J., Pedersen, J. S. & Otzen, D. E., Feb 2022, In: Communications Biology. 5, 1, 10 p., 123.

A Protein Corona Modulates Interactions of α-Synuclein with Nanoparticles and Alters the Rates of the Microscopic Steps of Amyloid Formation

Mohammad-Beigi, H., Zanganeh, M., Scavenius, C., Eskandari, H., Farzadfard, A., Shojaosadati, S. A., Enghild, J. J., Otzen, D. E., Buell, A. K. & Sutherland, D. S., Jan 2022, In: ACS Nano. 16, 1, p. 1102-1118 17 p.

Chaperones mainly suppress primary nucleation during formation of functional amyloid required for bacterial biofilm formation

Nagaraj, M., Najarzadeh, Z., Pansieri, J., Biverstål, H., Musteikyte, G., Smirnovas, V., Matthews, S., Emanuelsson, C., Johansson, J., Buxbaum, J. N., Morozova-Roche, L. & Otzen, D. E., Jan 2022, In: Chemical Science. 13, 2, p. 536-553 18 p.

Folding steps in the fibrillation of functional amyloid: denaturant sensitivity reveals common features in nucleation and elongation

Sønderby, T. V., Rasmussen, H. Ø., Frank, S. A., Skov Pedersen, J. & Otzen, D. E., Jan 2022, In: Journal of Molecular Biology. 434, 2, 18 p., 167337.

Characterization of exogenous αSN response genes and their relation to Parkinson's disease using network analyses

Nayeri, Z., Aliakbari, F., Afzali, F., Parsafar, S., Gharib, E., Otzen, D. E. & Morshedi, D., 2022, In: Frontiers in Pharmacology. 13, 14 p., 966760.

Interaction of membrane vesicles with the Pseudomonas functional amyloid protein FapC facilitates amyloid formation.

Najarzadeh, Z., Mohammad-Beigi, H., Pedersen, J. N., Christiansen, G., Pedersen, J. S., Nielsen, J. & Otzen, D. E., 2022, In: BBA advances. 2, p. 100055

Polarized α-synuclein trafficking and transcytosis across brain endothelial cells via Rab7-decorated carriers

Alam, P., Holst, M. R., Lauritsen, L., Nielsen, J., Nielsen, S. S. E., Jensen, P. H., Brewer, J. R., Otzen, D. E. & Nielsen, M. S., 2022, In: Fluids and Barriers of the CNS. 19, 1, 12 p., 37.

A semi high-throughput method for real-time monitoring of curli producing Salmonella biofilms on air-solid interfaces

Choong, F. X., Huzell, S., Rosenberg, M., Eckert, J. A., Nagaraj, M., Zhang, T., Melican, K., Otzen, D. E. & Richter-Dahlfors, A., Dec 2021, In: Biofilm. 3, 100060.

Lipid Peroxidation Products HNE and ONE Promote and Stabilize Alpha-Synuclein Oligomers by Chemical Modifications

Andersen, C., Grønnemose, A. L., Pedersen, J. N., Nowak, J. S., Christiansen, G., Nielsen, J., Mulder, F. A. A., Otzen, D. E. & Jørgensen, T. J. D., 30 Nov 2021, In: Biochemistry. 60, 47, p. 3644-3658 15 p.

Adsorption of azo dyes by a novel bio-nanocomposite based on whey protein nanofibrils and nano-clay: Equilibrium isotherm and kinetic modeling

Rahimi Aqdam, S., Otzen, D. E., Mahmoodi, N. M. & Morshedi, D., Nov 2021, In: Journal of Colloid and Interface Science. 602, p. 490-503

Membrane Structure of Aquaporin Observed with Combined Experimental and Theoretical Sum Frequency Generation Spectroscopy

Schmüser, L., Trefz, M., Roeters, S. J., Beckner, W., Pfaendtner, J., Otzen, D., Woutersen, S., Bonn, M., Schneider, D. & Weidner, T., Nov 2021, In: Langmuir : the ACS journal of surfaces and colloids. 37, 45, p. 13452-13459 8 p.

The bacterial amyloids phenol soluble modulins from staphylococcus aureus catalyze alpha-synuclein aggregation

Haikal, C., Pascual, L. O., Najarzadeh, Z., Bernfur, K., Svanbergsson, A., Otzen, D. E., Linse, S. & Li, J. Y., Nov 2021, In: International Journal of Molecular Sciences . 22, 21, 11594.

Microfluidics and the quantification of biomolecular interactions

Otzen, D. E., Buell, A. K. & Jensen, H., Oct 2021, In: Current Opinion in Structural Biology. 70, p. 8-15 8 p.

Cys-labelling kinetics of membrane protein GlpG: a role for specific SDS binding and micelle changes?

Otzen, D. E., Pedersen, J. N., Somavarapu, A. K., Clement, A., Ji, M., Petersen, E. H., Pedersen, J. S., Urban, S. & Schafer, N. P., Sept 2021, In: Biophysical Journal. 120, 18, p. 4115-4128

Identification of amyloidogenic proteins in the microbiomes of a rat Parkinson's disease model and wild-type rats

Christensen, L. F. B., Alijanvand, S. H., Burdukiewicz, M., Herbst, F-A., Kjeldal, H., Dueholm, M. S. & Otzen, D. E., Sept 2021, In: Protein Science. 30, 9, p. 1854-1870

Heparin promotes fibrillation of most phenol-soluble modulin virulence peptides from Staphylococcus aureus

Najarzadeh, Z., Zaman, M., Sereikaite, V., Strømgaard, K., Andreasen, M. & Otzen, D. E., Aug 2021, In: The Journal of Biological Chemistry. 297, 2, p. 100953 14 p.

Human Fibrinogen Inhibits Amyloid Assembly of Most Phenol-Soluble Modulins from Staphylococcus aureus

Najarzadeh, Z., Nielsen, J., Farzadfard, A., Sereikaite, V., Strømgaard, K., Meyer, R. L. & Otzen, D. E., Aug 2021, In: ACS Omega. 6, 34, p. 21960-21970 11 p.

Ubiquitin forms conventional decorated micelle structures with sodium dodecyl sulfate at saturation

Mortensen, H. G., Otzen, D. E. & Pedersen, J. S., Aug 2021, In: Journal of Colloid and Interface Science. 596, p. 233-244 12 p.

Multiple system atrophy-associated oligodendroglial protein p25α stimulates formation of novel α-synuclein strain with enhanced neurodegenerative potential

Ferreira, N., Gram, H., Sorrentino, Z. A., Gregersen, E., Schmidt, S. I., Reimer, L., Betzer, C., Perez-Gozalbo, C., Beltoja, M., Nagaraj, M., Wang, J., Nowak, J. S., Dong, M., Willén, K., Cholak, E., Bjerregaard-Andersen, K., Mendez, N., Rabadia, P., Shahnawaz, M., Soto, C., Otzen, D. E., Meyer, M., Giasson, B. I., Romero-Ramos, M. & Jensen, P. H., Jul 2021, In: Acta Neuropathologica. 142, p. 87-115

Per-glycosylation of the Surface-Accessible Lysines: One-Pot Aqueous Route to Stabilized Proteins with Native Activity

Walther, R., Monge, P., Pedersen, A., Benderoth, A., Pedersen, J., Farzadfard, A., Mandrup, O., Howard, K., Otzen, D. & Zelikin, A. N., Jul 2021, In: ChemBioChem. 22, 14, p. 2478-2485 8 p.

A multimethod approach for analyzing FapC fibrillation and determining mass per length

Rasmussen, H. Ø., Otzen, D. E. & Pedersen, J. S., 1 Jun 2021, In: Biophysical Journal. 120, 11, p. 2262-2275 14 p.

In situ Sub-Cellular Identification of Functional Amyloids in Bacteria and Archaea by Infrared Nanospectroscopy

Otzen, D. E., Dueholm, M. S., Najarzadeh, Z., Knowles, T. P. J. & Ruggeri, F. S., Jun 2021, In: Small Methods. 5, 6, 10 p., 2001002.

In-Silico Evidence for a Two Receptor Based Strategy of SARS-CoV-2

Milanetti, E., Miotto, M., Di Rienzo, L., Nagaraj, M., Monti, M., Golbek, T. W., Gosti, G., Roeters, S. J., Weidner, T., Otzen, D. E. & Ruocco, G., Jun 2021, In: Frontiers in Molecular Biosciences. 8, 11 p., 690655.

C subunit of the ATP synthase is an amyloidogenic calcium dependent channel-forming peptide with possible implications in mitochondrial permeability transition

Amodeo, G. F., Lee, B. Y., Krilyuk, N., Filice, C. T., Valyuk, D., Otzen, D. E., Noskov, S., Leonenko, Z. & Pavlov, E. V., Apr 2021, In: Scientific Reports. 11, 1, 10 p., 8744.

Multiple Protective Roles of Nanoliposome-Incorporated Baicalein against Alpha-Synuclein Aggregates

Aliakbari, F., Mohammad-Beigi, H., Abbasi, S., Rezaei-Ghaleh, N., Lermyte, F., Parsafar, S., Becker, S., Tafreshi, A. P., O'Connor, P. B., Collingwood, J. F., Christiansen, G., Sutherland, D. S., Jensen, P. H., Morshedi, D. & Otzen, D. E., 10 Feb 2021, In: Advanced Functional Materials. 31, 7, 2007765.

A Triple Role for a Bilayer: Using Nanoliposomes to Cross and Protect Cellular Membranes

Otzen, D. E., Morshedi, D., Mohammad-Beigi, H. & Aliakbari, F., Feb 2021, In: Journal of Membrane Biology. 254, 1, p. 29-39

Breakdown of supersaturation barrier links protein folding to amyloid formation

Noji, M., Samejima, T., Yamaguchi, K., So, M., Yuzu, K., Chatani, E., Akazawa-Ogawa, Y., Hagihara, Y., Kawata, Y., Ikenaka, K., Mochizuki, H., Kardos, J., Otzen, D. E., Bellotti, V., Buchner, J. & Goto, Y., Jan 2021, In: Communications Biology. 4, 1, 10 p., 120.

Molecular characteristics of porcine alpha-synuclein splicing variants

Larsen, K., Bæk, R., Sahin, C., Kjær, L., Christiansen, G., Nielsen, J., Farajzadeh, L. & Otzen, D. E., Jan 2021, In: Biochimie. 180, p. 121-133 13 p.

Driving forces in amyloidosis: How does a light chain make a heavy heart?

Otzen, D., 2021, In: The Journal of Biological Chemistry. 296, p. 100785 100785.

How epigallocatechin gallate binds and assembles oligomeric forms of human alpha-synuclein

Andersen, C. B., Yoshimura, Y., Nielsen, J., Otzen, D. E. & Mulder, F. A. A., 2021, In: The Journal of Biological Chemistry. 296, 10 p., 100788.

Phenol-Soluble Modulins Modulate Persister Cell Formation in Staphylococcus aureus

Baldry, M., Bojer, M. S., Najarzadeh, Z., Vestergaard, M., Meyer, R. L., Otzen, D. E. & Ingmer, H., Nov 2020, In: Frontiers in Microbiology. 11, 573253.

Inhibitors of α-Synuclein fibrillation and oligomer toxicity in Rosa damascena: the all-pervading powers of flavonoids and phenolic glycosides

Eskandari, H., Ghanadian, M., Noleto-Dias, C., Lomax, C., Tawfike, A., Christiansen, G., Sutherland, D. S., Ward, J. L., Mohammad-Beigi, H. & Otzen, D. E., Oct 2020, In: ACS Chemical Neuroscience. 11, 19, p. 3161–3173

DIBMA nanodiscs keep α-synuclein folded

Adão, R., Cruz, P. F., Vaz, D. C., Fonseca, F., Pedersen, J. N., Ferreira-da-Silva, F., Brito, R. M. M., Ramos, C. H. I., Otzen, D., Keller, S. & Bastos, M., Sept 2020, In: Biochimica et Biophysica Acta - Biomembranes. 1862, 9, 16 p., 183314.

Peak Force Infrared - Kelvin Probe Force Microscopy

Jakob, D. S., Wang, H., Zeng, G., Otzen, D. E., Yan, Y. & Xu, X., Sept 2020, In: Angewandte Chemie International Edition. 59, 37, p. 16083-16090 8 p.

Peroxynitrous acid (ONOOH) modifies the structure of anastellin and influences its capacity to polymerize fibronectin

He, J., Becares, E. R., Thulstrup, P. W., Gamon, L. F., Pedersen, J. N., Otzen, D., Gourdon, P., Davies, M. J. & Hägglund, P., Sept 2020, In: Redox Biology. 36, 101631.

Quantitating denaturation by formic acid: Imperfect repeats are essential to the stability of the functional amyloid protein FapC

Christensen, L. F. B., Nowak, J. S., Sønderby, T. V., Frank, S. A. & Otzen, D. E., Sept 2020, In: The Journal of biological chemistry. 295, 37, p. 13031–13046 16 p.

SDS-induced multi-stage unfolding of a small globular protein through different denatured states revealed by single-molecule fluorescence

Krainer, G., Hartmann, A., Bogatyr, V., Nielsen, J., Schlierf, M. & Otzen, D. E., Sept 2020, In: Chemical Science. 11, 34, p. 9141-9153 13 p.

Multi-Step Unfolding and Rearrangement of α-Lactalbumin by SDS Revealed by Stopped-Flow SAXS

Jensen, G. V., Pedersen, J. N., Otzen, D. E. & Pedersen, J. S., Jul 2020, In: Frontiers in Molecular Biosciences. 7, 125.

The status of the terminal regions of α-synuclein in different forms of aggregates during fibrillization

Marvian, A. T., Aliakbari, F., Mohammad-Beigi, H., Ahmadi, Z. A., Mehrpouyan, S., Lermyte, F., Nasouti, M., Collingwood, J. F., Otzen, D. E. & Morshedi, D., Jul 2020, In: International Journal of Biological Macromolecules. 155, p. 543-550 8 p.

Amyloid formation of α-synuclein based on the solubility- and supersaturation-dependent mechanism

Sawada, M., Yamaguchi, K., Hirano, M., Noji, M., So, M., Otzen, D. E., Kawata, Y. & Goto, Y., May 2020, In: Langmuir. 36, 17, p. 4671-4681

Nanosilver Mitigates Biofilm Formation via FapC Amyloidosis Inhibition

Huma, Z-E., Javed, I., Zhang, Z., Bilal, H., Sun, Y., Hussain, S. Z., Davis, T. P., Otzen, D. E., Landersdorfer, C. B., Ding, F., Hussain, I. & Ke, P. C., May 2020, In: Small (Weinheim an der Bergstrasse, Germany). 16, 21, 10 p., 1906674.

The hydrophobic effect characterises the thermodynamic signature of amyloid fibril growth

van Gils, J. H. M., van Dijk, E., Peduzzo, A., Hofmann, A., Vettore, N., Schützmann, M. P., Groth, G., Mouhib, H., Otzen, D. E., Buell, A. K. & Abeln, S., May 2020, In: PLOS Computational Biology. 16, 5, e1007767.

Novel noscapine derivatives stabilize the native state of insulin against fibrillation

Alijanvand, S. H., Christensen, M. H., Christiansen, G., Harikandei, K. B., Salehi, P., Schiøtt, B., Moosavi-Movahedi, A. A. & Otzen, D. E., Mar 2020, In: International Journal of Biological Macromolecules. 147, p. 98-108 11 p.

Unfolding and partial refolding of a cellulase from the SDS-denatured state: From β-sheet to α-helix and back

Rasmussen, H. Ø., Enghild, J. J., Otzen, D. E. & Pedersen, J. S., Jan 2020, In: B B A - General Subjects. 1864, 1, p. 129434 1-12 12 p., 129434.

A complete picture of protein unfolding and refolding in surfactants

Pedersen, J. N., Lyngsø, J., Zinn, T., Otzen, D. & Pedersen, J. S., 2020, In: Chemical Science. 11, 3, p. 699-712 14 p.

Accelerated Amyloid Beta Pathogenesis by Bacterial Amyloid FapC

Javed, I., Zhang, Z., Adamcik, J., Andrikopoulos, N., Li, Y., Otzen, D. E., Lin, S., Mezzenga, R., Davis, T. P., Ding, F. & Ke, P. C., 2020, In: Advanced Science. 7, 18, 15 p., 2001299.

Amyloid fibril inhibition, acceleration, or fragmentation: Are nano-based approaches advance in the right direction?

Hajipour, M. J., Mohammad-Beigi, H., Nabipour, I., Mahmoudi, N., Azhdarzadeh, M., Derakhshankhah, H., El Dawud, D., Mohammadinejad, R. & Otzen, D., 2020, In: Nano Today. 35, December, 5 p., 100983.

Predicted Loop Regions Promote Aggregation: A Study of Amyloidogenic Domains in the Functional Amyloid FapC

Nagaraj, M., Ahmed, M., Lyngsø, J., Vad, B. S., Bøggild, A., Fillipsen, A., Pedersen, J. S., Otzen, D. E. & Akbey, Ü., 2020, In: Journal of Molecular Biology. 432, 7, p. 2232-2252 21 p.

Size-Selective Phagocytic Clearance of Fibrillar α-Synuclein through Conformational Activation of Complement Receptor 4

Juul-Madsen, K., Qvist, P., Bendtsen, K. L., Langkilde, A. E., Vestergaard, B., Howard, K., Dehesa-Etxebeste, M., Paludan, S. R., Andersen, G. R., Jensen, P. H., Otzen, D. E., Romero-Ramos, M. & Vorup-Jensen, T., 2020, In: Journal of Immunology. 204, 5, p. 1345-1361 17 p.

The interactome of stabilized α-synuclein oligomers and neuronal proteins

van Diggelen, F., Frank, S. A., Somavarapu, A. K., Scavenius, C., Apetri, M. M., Nielsen, J., Tepper, A. W. J. W., Enghild, J. J. & Otzen, D. E., 2020, In: The FEBS Journal. 287, 10, p. 2037-2054 18 p.

Bacterial amphiphiles as amyloid inducers: Effect of Rhamnolipid and Lipopolysaccharide on FapC fibrillation

Najarzadeh, Z., Pedersen, J. N., Christiansen, G., Shojaosadati, S. A., Pedersen, J. S. & Otzen, D. E., 1 Nov 2019, In: B B A - Proteins and Proteomics. 1867, 11, 140263.

Plant polyphenols inhibit functional amyloid and biofilm formation in Pseudomonas strains by directing monomers to off-pathway oligomers

Najarzadeh, Z., Mohammad-Beigi, H., Pedersen, J. N., Christiansen, G., Sønderby, T. V., Shojaosadati, S. A., Morshedi, D., Strømgaard, K., Meisl, G., Sutherland, D., Pedersen, J. S. & Otzen, D., 1 Nov 2019, In: Biomolecules. 9, 11, 659.

Alterations in Blood Monocyte Functions in Parkinson's Disease

Nissen, S. K., Shrivastava, K., Schulte, C., Otzen, D. E., Goldeck, D., Berg, D., Møller, H. J., Maetzler, W. & Romero-Ramos, M., Nov 2019, In: Movement Disorders. 34, 11, p. 1711-1721 11 p.

Molecular dynamics study of ACBP denaturation in alkyl sulfates demonstrates possible pathways of unfolding through fused surfactant clusters

Poghosyan, A. H., Schafer, N., Lyngsø, J., Shahinyan, A. A., Pedersen, J. S. & Otzen, D., Apr 2019, In: Protein Engineering, Design and Selection. 32, 4, p. 175-190 16 p., gzz037.

Conservation of the Amyloid Interactome Across Diverse Fibrillar Structures

Juhl, D. W., Risør, M. W., Scavenius, C., Rasmussen, C. B., Otzen, D., Nielsen, N. C. & Enghild, J. J., 7 Mar 2019, In: Scientific Reports. 9, 1, 14 p., 3863.

Imperfect repeats in the functional amyloid protein FapC reduce the tendency to fragment during fibrillation

Rasmussen, C. B., Christiansen, G., Vad, B. S., Lynggaard, C., Enghild, J. J., Andreasen, M. & Otzen, D., 1 Mar 2019, In: Protein Science. 28, 3, p. 633-642 10 p.

Two conformationally distinct α-synuclein oligomers share common epitopes and the ability to impair long-term potentiation

van Diggelen, F., Hrle, D., Apetri, M., Christiansen, G., Rammes, G., Tepper, A. & Otzen, D. E., Mar 2019, In: PLOS ONE. 14, 3, 22 p., 0213663.

Reducing the Amyloidogenicity of Functional Amyloid Protein FapC Increases Its Ability To Inhibit α-Synuclein Fibrillation

Christensen, L. F. B., Jensen, K. F., Nielsen, J., Vad, B. S., Christiansen, G. & Otzen, D. E., Feb 2019, In: ACS Omega. 4, 2, p. 4029-4039 11 p.

Physical Determinants of Amyloid Assembly in Biofilm Formation

Andreasen, M., Meisl, G., Taylor, J. D., Michaels, T. C. T., Levin, A., Otzen, D. E., Chapman, M. R., Dobson, C. M., Matthews, S. J. & Knowles, T. P. J., 8 Jan 2019, In: mBio. 10, 1, 12 p., e02279-18.

A Possible Connection Between Plant Longevity and the Absence of Protein Fibrillation: Basis for Identifying Aggregation Inhibitors in Plants

Mohammad-Beigi, H., Kjær, L., Eskandari, H., Aliakbari, F., Christiansen, G., Ruvo, G., L. Ward, J. & Otzen, D., 2019, In: Frontiers in Plant Science. 10, 148, 15 p., 148.

Functional Amyloids

Otzen, D. & Riek, R., 2019, In: Cold Spring Harbor Perspectives in Biology. 11, 12, 30 p.

In vitro and in silico assessment of the developability of a designed monoclonal antibody library

Wolf Pérez, A-M., Sormanni, P., Andersen, J. S., Sakhnini, L. I., Rodriguez-Leon, I., Bjelke, J. R., Gajhede, A. J., De Maria, L., Otzen, D. E., Vendruscolo, M. & Lorenzen, N., 2019, In: mAbs. 11, 2, p. 388-400 13 p.

Lysophospholipids induce fibrillation of the repeat domain of Pmel17 through intermediate core-shell structures

Pedersen, J. N., Jiang, Z., Christiansen, G., Lee, J. C., Pedersen, J. S. & Otzen, D. E., 2019, In: Biochimica et Biophysica Acta - Proteins and Proteomics. 1867, 5, p. 519-528 10 p.

Mechanistic Understanding of the Interactions between Nano-Objects with Different Surface Properties and α-Synuclein

Mohammad-Beigi, H., Hosseini, A., Adeli, M., Ejtehadi, M. R., Christiansen, G., Sahin, C., Tu, Z., Tavakol, M., Dilmaghani-Marand, A., Nabipour, I., Farzadfar, F., Otzen, D. E., Mahmoudi, M. & Hajipour, M. J., 2019, In: ACS Nano. 13, 3, p. 3243-3256 14 p.

Oleuropein derivatives from olive fruit extracts reduce α-synuclein fibrillation and oligomer toxicity

Mohammad-Beigi, H., Aliakbari, F., Sahin, C., Lomax, C., Tawfike, A., P. Schafer, N., Amiri-Nowdijeh, A., Eskandari, H., Møller, I. M., Hosseini-Mazinani, M., Christiansen, G., Ward, J. L., Morshedi, D. & Otzen, D., 2019, In: Journal of Biological Chemistry. 294, 11, p. 4215-4232 18 p.

Can a Charged Surfactant Unfold an Uncharged Protein?

Højgaard, C., Sørensen, H. V., Pedersen, J. S., Winther, J. R. & Otzen, D. E., 4 Dec 2018, In: Biophysical Journal. 115, 11, p. 2081-2086

The Use of Surfactants to Solubilise a Glucagon Analogue

Madsen, J. K., Giehm, L. & Otzen, D. E., Dec 2018, In: Pharmaceutical Research. 35, 12, 235.

Potent α-Synuclein Aggregation Inhibitors, Identified by High-Throughput Screening, Mainly Target the Monomeric State

Kurnik, M., Sahin, C., Andersen, C. B., Lorenzen, N., Giehm, L., Mohammad-Beigi, H., Jessen, C. M., Pedersen, J. S., Christiansen, G., Petersen, S. V., Staal, R., Krishnamurthy, G., Pitts, K., Reinhart, P. H., Mulder, F. A. A., Mente, S., Hirst, W. D. & Otzen, D. E., 15 Nov 2018, In: Cell Chemical Biology. 25, 11, p. 1389-1402

Protein Engineering Reveals Mechanisms of Functional Amyloid Formation in Pseudomonas aeruginosa Biofilms

Bleem, A., Christiansen, G., Madsen, D. J., Maric, H., Strømgaard, K., Bryers, J. D., Daggett, V., Meyer, R. L. & Otzen, D. E., 12 Oct 2018, In: Journal of Molecular Biology. 430, 20, p. 3751-3763

Using Liprotides to Deliver Cholesterol to the Plasma Membrane

Frislev, H. S., Nielsen, J., Nylandsted, J. & Otzen, D., Aug 2018, In: Journal of Membrane Biology. 25, 4, p. 581-592 12 p.

Early events in copper-ion catalyzed oxidation of α-synuclein

Tiwari, M. K., Leinisch, F., Sahin, C., Møller, I. M., Otzen, D., Davies, M. J. & Bjerrum, M. J., Jun 2018, In: Free Radical Biology & Medicine. 121, p. 38-50

The potential of zwitterionic nanoliposomes against neurotoxic alpha-synuclein aggregates in Parkinson's Disease

Aliakbari, F., Mohammad-Beigi, H., Rezaei-Ghaleh, N., Becker, S., Dehghani Esmatabad, F., Eslampanah Seyedi, H. A., Bardania, H., Tayaranian Marvian, A., Collingwood, J. F., Christiansen, G., Zweckstetter, M., Otzen, D. E. & Morshedi, D., 17 May 2018, In: Nanoscale. 10, 19, p. 9174-9185 12 p.

High-quality draft genome sequence of Sphaerisporangium cinnabarinum ATCC 31213

Christensen, L. F. B., Otzen, D. & Dueholm, M. S., 1 May 2018, In: Genome Announcements. 6, 21, 2 p., e00456-18.

Pseudomonas aeruginosa rhamnolipid induces fibrillation of human α-synuclein and modulates its effect on biofilm formation

Andersen, K. K., Vad, B. S., Kjaer, L., Tolker-Nielsen, T., Christiansen, G. & Otzen, D. E., May 2018, In: FEBS Letters. 592, 9, p. 1484-1496

Corneal dystrophy mutations drive pathogenesis by targeting TGFBIp stability and solubility in a latent amyloid-forming domain

Stenvang, M., Schafer, N. P., Malmos, K. G., Pérez, A-M. W., Niembro, O., Sormanni, P., Basaiawmoit, R. V., Christiansen, G., Andreasen, M. & Otzen, D. E., 13 Apr 2018, In: Journal of Molecular Biology. 430, 8, p. 1116-1140

Stabilizing vitamin D3 using the molten globule state of α-lactalbumin

Pedersen, J. N., Sørensen, H. V. & Otzen, D. E., Mar 2018, In: Journal of Dairy Science. 101, 3, p. 1817–1826

Role of charge and hydrophobicity in liprotide formation: a molecular dynamics study with experimental constraints

Nedergaard Pedersen, J., Wilhelmus Johannes Maria Frederix, P., Skov Pedersen, J., Marrink, S. J. & Otzen, D., 2 Feb 2018, In: ChemBioChem. 19, 3, p. 263–271

Liprotides assist in folding of outer membrane proteins

Nedergaard Pedersen, J., Skov Pedersen, J. & Otzen, D. E., 1 Feb 2018, In: Protein Science. 27, 2, p. 451-462 12 p.

Dynamic content exchange between liprotides

Frislev, H. S., Jakobsen, S. C. L., Frank, S. A. & Otzen, D. E., Feb 2018, In: Biophysical Chemistry. 233, p. 13-18 6 p.

Formulation and anti-neurotoxic activity of baicalein-incorporating neutral nanoliposome

Aliakbari, F., Shabani, A. A., Bardania, H., Mohammad-Beigi, H., Tayaranian Marvian, A., Dehghani Esmatabad, F., Vafaei, A. A., Shojaosadati, S. A., Saboury, A. A., Christiansen, G., Otzen, D. E. & Morshedi, D., 1 Jan 2018, In: Colloids and surfaces. B, Biointerfaces. 161, p. 578-587 10 p.

The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein

Christensen, L. F. B., Hansen, L. M., Finster, K., Christiansen, G., Nielsen, P. H., Otzen, D. E. & Dueholm, M. S., 2018, In: Frontiers in Microbiology. 9, 11 p., 2729.

α-synucleins from animal species show low fibrillation propensities and weak oligomer membrane disruption

Sahin, C., Kjær, L., Christensen, M. S., Nedergaard Pedersen, J., Christiansen, G., Pérez, A-M. W., Moller, I. M., Enghild, J., Pedersen, J. S., Larsen, K. & Otzen, D. E., 2018, In: Biochemistry. 57, 34, p. 5145-5158 14 p.

Myoglobin and α-Lactalbumin Form Smaller Complexes with the Biosurfactant Rhamnolipid Than with SDS

Mortensen, H. G., Madsen, J. K., Andersen, K. K., Vosegaard, T., Deen, G. R., Otzen, D. E. & Pedersen, J. S., 19 Dec 2017, In: Biophysical Journal. 113, 12, p. 2621-2633 13 p.

Liprotides kill cancer cells by disrupting the plasma membrane

Frislev, H. S., Boye, T. L., Nylandsted, J. & Otzen, D., 9 Nov 2017, In: Scientific Reports. 7, 15129.

Correction to: How glycosaminoglycans promote fibrillation of salmon calcitonin

Malmos, K. G., Bjerring, M., Jessen, C. M., Nielsen, E. H. T., Poulsen, E. T., Christiansen, G., Vosegaard, T., Skrydstrup, T., Enghild, J. J., Pedersen, J. S. & Otzen, D. E., 22 Sept 2017, In: Journal of Biological Chemistry. 292, 38, p. 15992

The Changing Face of Aging: Highly Sulfated Glycosaminoglycans Induce Amyloid Formation in a Lattice Corneal Dystrophy Model Protein

Malmos, K. G., Stenvang, M., Sahin, C., Christiansen, G. & Otzen, D. E., 1 Sept 2017, In: Journal of Molecular Biology. 429, 18

Glycolipid Biosurfactants Activate, Dimerize, and Stabilize Thermomyces lanuginosus Lipase in a pH-Dependent Fashion

Madsen, J. K., Kaspersen, J. D., Andersen, C. B., Nedergaard Pedersen, J., Andersen, K. K., Pedersen, J. S. & Otzen, D. E., 15 Aug 2017, In: Biochemistry. 56, 32, p. 4256-4268 13 p.

Critical Influence of Cosolutes and Surfaces on the Assembly of Serpin-Derived Amyloid Fibrils

Risør, M. W., Juhl, D. W., Bjerring, M., Mathiesen, J., Enghild, J. J., Nielsen, N. C. & Otzen, D. E., 8 Aug 2017, In: Biophysical Journal. 113, 3, p. 580-596 17 p.

α-Synuclein Oligomers: A Study in Diversity

van Diggelen, F., Tepper, A., Petri, M. & Otzen, D., Jul 2017, In: Israel Journal of Chemistry. 57, 7-8, p. 699-723

Tailoring thermal treatment to form liprotide complexes between oleic acid and different proteins

Sørensen, H. V., Pedersen, J. N., Pedersen, J. S. & Otzen, D. E., Jun 2017, In: BBA Proteins and Proteomics. 1865, 6, p. 682–693

Refolding of SDS-Unfolded Proteins by Nonionic Surfactants

Kaspersen, J. D., Søndergaard, A., Madsen, D. J., Otzen, D. E. & Pedersen, J. S., 25 Apr 2017, In: Biophysical Journal. 112, 8, p. 1609-1620 12 p.

Biosurfactants and surfactants interacting with membranes and proteins: Same but different?

Otzen, D., Apr 2017, In: BBA Biomembranes. 1859, 4, p. 639–649

ThT 101: a primer on the use of thioflavin T to investigate amyloid formation

Malmos, K., Blancas-Mejia, L. M., Weber, B., Buchner, J., Ramirez-Alvarado, M., Naiki, H. & Otzen, D., Apr 2017, In: Amyloid: the Journal of Protein Folding Disorders. 24, 1, p. 1-16 16 p.

The random co-polymer glatiramer acetate rapidly kills primary human leukocytes through sialic-acid-dependent cell membrane damage

Christiansen, S. H., Zhang, X., Juul-Madsen, K., Hvam, M. L., Vad, B. S., Behrens, M. A., Thygesen, I. L., Jalilian, B., Pedersen, J. S., Howard, K., Otzen, D. E. & Vorup-Jensen, T., Mar 2017, In: B B A - Biomembranes. 1859, 3, p. 425-437 13 p.

Antibodies against the C-terminus of α-synuclein modulate its fibrillation

Sahin, C., Lorenzen, N., Lemminger, L., Christiansen, G., Møller, I. M., Vesterager, L. B., Pedersen, L. Ø., Fog, K., Kallunki, P. & Otzen, D. E., Jan 2017, In: Biophysical Chemistry. 220, p. 34-41

A new class of hybrid secretion system is employed in Pseudomonas amyloid biogenesis

Rouse, S. L., Hawthorne, W. J., Berry, J-L., Chorev, D. S., Ionescu, S. A., Lambert, S., Stylianou, F., Ewert, W., Mackie, U., Morgan, R. M. L., Otzen, D., Herbst, F-A., Nielsen, P. H., Dueholm, M., Bayley, H., Robinson, C. V., Hare, S. & Matthews, S., 2017, In: Nature Communications. 8, 1, 263.

Human lysozyme peptidase resistance is perturbed by the anionic glycolipid biosurfactant rhamnolipid produced by the opportunistic pathogen Pseudomonas aeruginosa

Andersen, K. K., Vad, B. S., Scavenius, C., Enghild, J. J. & Otzen, D. E., 2017, In: Biochemistry. 56, 1, p. 260–270

Epigallocatechin Gallate Remodels overexpressed Functional Amyloids in Pseudomonas aeruginosa and Increases Biofilm Susceptibility to Antibiotic Treatment

Stenvang, M., Dueholm, M. S., Vad, B. S., Seviour, T. W., Zeng, G., Geifman-Shochat, S., Søndergaard, M. T., Christiansen, G., Meyer, R. L., Kjelleberg, S., Otzen, D. E. & Nielsen, P. H., 16 Dec 2016, In: Journal of Biological Chemistry. 291, 51, p. 26540-26553

Alpha-synuclein and familial variants affect the chain order and the thermotropic phase behavior of anionic lipid vesicles

Pantusa, M., Vad, B., Lillelund, O., Kjær, L., Otzen, D. & Bartucci, R., Sept 2016, In: B B A - Proteins and Proteomics. 1864, p. 1206-1214

Using protein-fatty acid complexes to improve vitamin D stability

Pedersen, J. N., Frislev, H. K. S., Pedersen, J. S. & Otzen, D. E., Sept 2016, In: Journal of Dairy Science. 99, 10, p. 7755–7767

How Glycosaminoglycans Promote Fibrillation of Salmon Calcitonin

Malmos, K. G., Bjerring, M., Jessen, C. M., Nielsen, E. H. T., Poulsen, E. T., Christiansen, G., Vosegaard, T., Skrydstrup, T., Enghild, J. J., Pedersen, J. S. & Otzen, D. E., 5 Aug 2016, In: Journal of Biological Chemistry. 291, 32, p. 16849-16862 31 p.

The neural chaperone proSAAS blocks α-synuclein fibrillation and neurotoxicity

Jarvela, T. S., Lam, H. A., Helwig, M., Lorenzen, N., Otzen, D. E., McLean, P. J., Maidment, N. T. & Lindberg, I., 25 Jul 2016, In: Proceedings of the National Academy of Sciences. 113, 32

A Complex Dance: The Importance of Glycosaminoglycans and Zinc in the Aggregation of Human Prolactin

Christensen, L. F. B., Malmos, K. G., Christiansen, G. & Otzen, D. E., 22 Jun 2016, In: Biochemistry. 55, 26, p. 3674–3684 11 p.

Incorporation of β-Silicon-β3-Amino Acids in the Antimicrobial Peptide Alamethicin Provides a 20-Fold Increase in Membrane Permeabilization

Madsen, J. L. H., Hjørringgaard, C. U., Vad, B. S., Otzen, D. & Skrydstrup, T., 6 Jun 2016, In: Chemistry: A European Journal. 22, 24, p. 8358-8367 10 p.

α-Synuclein vaccination modulates regulatory T cell activation and microglia in the absence of brain pathology

Christiansen, J. R., Olesen, M. N., Otzen, D. E., Romero-Ramos, M. & Sanchez-Guajardo, V., 9 Apr 2016, In: Journal of Neuroinflammation. 13, 74.

Epigallocatechin gallate remodels fibrils of Lattice Corneal Dystrophy protein, facilitating proteolytic degradation and preventing formation of membrane-permeabilizing species

Stenvang, M., Christiansen, G. & Otzen, D. E., 4 Apr 2016, In: Biochemistry. 55, 16, p. 2344-2357

The compact and biologically relevant structure of inter-α-inhibitor is maintained by the chondroitin sulfate chain and divalent cations

Scavenius, C., Nikolajsen, C. L., Stenvang, M., Thøgersen, I., Wyrozemski, L., Wisniewski, H-G., Otzen, D. E., Sanggaard, K. W. & Enghild, J. J., 26 Feb 2016, In: Journal of Biological Chemistry. 291, 9, p. 4658-4670 13 p.

Detection of proteolytic signatures for Parkinson's disease

Jordal, P. L., Dyrlund, T. F., Winge, K., Larsen, M. R., Danielsen, E. H., Wells, J. A., Otzen, D. E. & Enghild, J. J., 1 Feb 2016, In: Future Neurology. 11, 1, p. 15-32 18 p.

A Monte Carlo Study of the Early Steps of Functional Amyloid Formation

Tian, P., Lindorff-Larsen, K., Boomsma, W., Jensen, M. H. & Otzen, D. E., 8 Jan 2016, In: PLOS ONE. 11, 1

Concatemers of Outer Membrane Protein A Take Detours in the Folding Landscape

Andersen, K. K., Vad, B., Omer, S. & Otzen, D. E., 2016, In: Biochemistry. 55, 51, p. 7123–7140 7 p.

Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11

Kim, J-Y., Sahu, S., Yau, Y-H., Wang, X., Shochat, S. G., Nielsen, P. H., Dueholm, M. S., Otzen, D. E., Lee, J., Delos Santos, M. M. S., Yam, J. K. H., Kang, N-Y., Park, S-J., Kwon, H., Seviour, T. W., Yang, L., Givskov, M. & Chang, Y-T., 2016, In: Journal of the American Chemical Society. p. 402-407

Formation and Characterization of α-Synuclein Oligomers

Paslawski, W., Lorenzen, N. & Otzen, D. E., 2016, In: Methods in molecular biology (Clifton, N.J.). 1345, p. 133-150 18 p.

Gallic acid loaded onto polyethylenimine-coated human serum albumin nanoparticles (PEI-HSA-GA NPs) stabilizes α-synuclein in the unfolded conformation and inhibits aggregation

Mohammad-Beigi, H., Morshedi, D., Shojaosadati, S. A., Pedersen, J. N., Marvian, A. T., Aliakbari, F., Christiansen, G., Pedersen, J. S. & Otzen, D. E., 2016, In: RSC Advances. 6, 88, p. 85312-85323 12 p.

Liprotides made of α-lactalbumin and cis fatty acids form core-shell and multi-layer structures with a common membrane-targeting mechanism

Frislev, H. K. S., Jessen, C. M., Oliveira, C. L. P., Pedersen, J. S. & Otzen, D. E., 2016, In: B B A - Proteins and Proteomics. 1864, 7, p. 847-859 13 p.

Near-complete 1H, 13C, 15N resonance assignments of dimethylsulfoxide-denatured TGFBIp FAS1-4 A546T

Kulminskaya, N. V., Yoshimura, Y., Runager, K., Sørensen, C. S., Bjerring, M., Andreasen, M., Otzen, D. E., Enghild, J. J., Nielsen, N. C. & Mulder, F. A. A., 2016, In: Biomolecular N M R Assignments. 10, 1, p. 25-29 5 p.

Structure, Aggregation, and Activity of a Covalent Insulin Dimer Formed During Storage of Neutral Formulation of Human Insulin

Hjorth, C. F., Norrman, M., Wahlund, P-O., Benie, A. J., Petersen, B. O., Jessen, C. M., Pedersen, T. Å., Vestergaard, K., Steensgaard, D. B., Pedersen, J. S., Naver, H., Hubálek, F., Poulsen, C. & Otzen, D., 2016, In: Journal of Pharmaceutical Sciences. 105, 4, p. 1376-1386 11 p.

The transcriptional regulator GalR self-assembles to form highly regular tubular structures

Agerschou, E. D., Christiansen, G., Schafer, N. P., Madsen, D. J., Brodersen, D. E., Semsey, S. & Otzen, D. E., 2016, In: Scientific Reports. 6, 27672.

Topological constraints and modular structure in the folding and functional motions of GlpG, an intramembrane protease

Schafer, N., Truong, H. H., Otzen, D., Lindorff-Larsen, K. & Wolynes, P. G., 2016, In: Proceedings of the National Academy of Sciences. 113, 8, p. 2098-2103

Weak and Saturable Protein-Surfactant Interactions in the Denaturation of Apo-α-Lactalbumin by Acidic and Lactonic Sophorolipid

Andersen, K. K., Vad, B. S., Roelants, S., van Bogaert, I. N. A. & Otzen, D. E., 2016, In: Frontiers in Microbiology. 7, 1711.

Promoting protein self-association in non-glycosylated Thermomyces lanuginosus lipase based on crystal lattice contacts

Madsen, J., Sørensen, T. R., Kaspersen, J. D., Silow, M. B., Vind, J., Pedersen, J. S., Svendsen, A. & Otzen, D. E., Dec 2015, In: BBA Proteins and Proteomics. 154, 12, p. 1914-1921 8 p.

Scaffolded multimers of hIAPP20-29 peptide fragments fibrillate faster and lead to different fibrils compared to the free hIAPP20-29 peptide fragment

Christoffersen, H. F., Andreasen, M., Zhang, S., Nielsen, E. H., Christiansen, G., Dong, M., Skrydstrup, T. & Otzen, D. E., Dec 2015, In: B B A - Proteins and Proteomics. 1854, 12, p. 1890-1897 8 p.

Strong interactions with polyethylenimine-coated human serum albumin nanoparticles (PEI-HSA NPs) alter α-synuclein conformation and aggregation kinetics

Mohammad-Beigi, H., Shojaosadati, S. A., Marvian, A. T., Pedersen, J. N., Klausen, L. H., Christiansen, G., Pedersen, J. S., Dong, M., Morshedi, D. & Otzen, D. E., 9 Nov 2015, In: Nanoscale. 7, p. 19627-19640 14 p.

Sucrose prevents protein fibrillation through compaction of the tertiary structure but hardly affects the secondary structure

Estrela, N., Franquelim, H. G., Lopes, C., Tavares, E., Macedo, J. A., Christiansen, G., Otzen, D. E. & Melo, E. P., Nov 2015, In: Proteins: Structure, Function, and Bioinformatics. 83, 11, p. 2039-2051 14 p.

The length distribution of frangible biofilaments

Michaels, T. C. T., Yde, P., Willis, J. C. W., Jensen, M. H., Otzen, D., Dobson, C. M., Buell, A. K. & Knowles, T. P. J., 28 Oct 2015, In: Journal of Chemical Physics. 143, 16, p. 1-15 15 p., 164901.

Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness

Zeng, G., Vad, B. S., Dueholm, M. S., Christiansen, G., Nilsson, M., Tolker-Nielsen, T., Nielsen, P. H., Meyer, R. L. & Otzen, D. E., 7 Oct 2015, In: Frontiers in Microbiology. 6, p. 1099

Interactions between misfolded protein oligomers and membranes: A central topic in neurodegenerative diseases?

Andreasen, M., Lorenzen, N. & Otzen, D., Sept 2015, In: B B A - Molecular and Cell Biology of Lipids. 1848, 9, p. 1897-1907

The natural, peptaibolic peptide SPF-5506-A4 adopts a β-bend spiral structure, shows low hemolytic activity and targets membranes through formation of large pores

Frahm, H., Hansen, S. K., Vad, B. S., Nielsen, E. H., Nielsen, J. T., Vosegaard, T., Skrydstrup, T. & Otzen, D. E., Aug 2015, In: B B A - Proteins and Proteomics. 1854, 8, p. 882-889 8 p.

The Use of Liprotides To Stabilize and Transport Hydrophobic Molecules

Pedersen, J. N., Pedersen, J. S. & Otzen, D. E., 28 Jul 2015, In: Biochemistry. 54, 31, p. 4815-4823 9 p.

The Tubular Sheaths Encasing Methanosaeta thermophila Filaments are Functional Amyloids

Dueholm, M. S., Larsen, P., Finster, K., Stenvang, M. R., Christiansen, G., Vad, B. S., Bøggild, A., Otzen, D. E. & Halkjær Nielsen, P., 24 Jun 2015, In: Journal of Biological Chemistry. 290, p. 20590-26600 11 p.

Proteins in a brave new surfactant world

Otzen, D. E., 1 Jun 2015, In: Current Opinion in Colloid & Interface Science. 20, 3, p. 161-169 9 p.

Phospholipid Ether Linkages Significantly Modulate the Membrane Affinity of the Antimicrobial Peptide Novicidin

Vad, B. S., Balakrishnan, V. S., Nielsen, S. B. & Otzen, D., Jun 2015, In: Journal of Membrane Biology. p. 487-496 10 p.

Purification and Identification of High Molecular Weight Products Formed During Storage of Neutral Formulation of Human Insulin

Hjorth, C. F., Hubálek, F., Andersson, J., Poulsen, C., Otzen, D. & Naver, H., Jun 2015, In: Pharmaceutical Research. 32, 6, p. 2072-2085 14 p.

The anionic biosurfactant rhamnolipid does not denature industrial enzymes

Madsen, J., Pihl, R., Møller, A. H., Tranberg Madsen, A., Otzen, D. & Andersen, K. K., 17 Apr 2015, In: Frontiers in Microbiology. 6, 13 p., 292.

A monomer-trimer model supports intermittent glucagon fibril growth

Košmrlj, A., Cordsen, P., Kyrsting, A., Otzen, D., Oddershede, L. B. & Jensen, M. H., 11 Mar 2015, In: Scientific Reports. 5, p. 1-6 6 p., 9005.

Protein aggregation: Close encounters of the greasy kind

Otzen, D., 17 Feb 2015, In: Nature Chemical Biology. 11, 3, p. 176-177 2 p.

Cooperative folding of a polytopic α-helical membrane protein involves a compact N-terminal nucleus and nonnative loops

Paslawski, W., Lillelund, O. K., Kristensen, J. V., Schafer, N. P., Baker, R. P., Urban, S. & Otzen, D. E., 10 Feb 2015, In: Proceedings of the National Academy of Sciences. 112, 6, p. 7978-7983 6 p.

Functional Amyloids Keep Quorum Sensing Molecules in Check

Seviour, T., Hansen, S. H., Yang, L., Yau, Y. H., Wang, V. B., Stenvang, M. R., Christiansen, G., Marsili, E., Givskov, M., Chen, Y., Otzen, D., Nielsen, P. H., Shochat, S. G., Kjelleberg, S. & Dueholm, M. S., 13 Jan 2015, In: Journal of Biological Chemistry. 290, p. 6457-6469 13 p.

Structure of a Functional Amyloid Protein Subunit Computed Using Sequence Variation

Tian, P., Boomsma, W., Wang, Y., Otzen, D., Jensen, M. H. & Lindorff-Larsen, K., 2015, In: Journal of the American Chemical Society. 137, 1, p. 22-25 4 p.

Folding energetics and oligomerization of polytopic α-helical transmembrane proteins

Neumann, J., Klein, N., Otzen, D. & Schneider, D., 15 Dec 2014, In: Archives of Biochemistry and Biophysics. 564, p. 281–296 16 p.

Membrane protein folding and stability

Otzen, D., 15 Dec 2014, In: Archives of Biochemistry and Biophysics. 564, p. 262-264 3 p.

Denaturation of α-lactalbumin and myoglobin by the anionic biosurfactant rhamnolipid

Andersen, K. K. & Otzen, D., Dec 2014, In: B B A - Bioenergetics. 1844, 12, p. 2338-2345 8 p.

Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins

Kaspersen, J. D., Pedersen, J. N., Hansted, J. G., Nielsen, S. B., Sakthivel, S., Wilhelm, K., Nemashkalova, E. L., Permyakov, S. E., Permyakov, E. A., Pinto Oliveira, C. L., Morozova-Roche, L. A., Otzen, D. & Pedersen, J. S., Dec 2014, In: ChemBioChem. 10, 18, p. 2693-2702 10 p.

The Antimicrobial Mechanism of Action of Epsilon-Poly-L-Lysine

Hyldgaard, M., Mygind, T., Vad, B. S., Stenvang, M., Otzen, D. & Meyer, R. L., Dec 2014, In: Applied and Environmental Microbiology. 80, 24, p. 7758-7770 12 p.

The Importance of Being Capped: Terminal Capping of an Amyloidogenic Peptide Affects Fibrillation Propensity and Fibril Morphology

Andreasen, M., Skeby, K. K., Zhang, S., Nielsen, E. H., Klausen, L. H., Frahm, H., Christiansen, G., Skrydstrup, T., Dong, M., Schiøtt, B. & Otzen, D., 21 Oct 2014, In: Biochemistry. 53, 44, p. 6968-6980 13 p.

High stability and cooperative unfolding of cytotoxic α-synuclein oligomers

Paslawski, W., Andreasen, M., Nielsen, S. B., Lorenzen, N., Thomsen, K., Kaspersen, J. D., Pedersen, J. S. & Otzen, D., 12 Sept 2014, In: Biochemistry. 53, 39, p. 6252-6263 12 p.

Low-Resolution Structures of OmpA⋅DDM Protein-Detergent Complexes

Kaspersen, J., Moestrup Jessen, C., Stougaard Vad, B., Skipper Sørensen, E., Kleiner Andersen, K., Glasius, M., Pinto Oliveira, C. L., Otzen, D. & Pedersen, J. S., 19 Aug 2014, In: ChemBioChem. 15, 14, p. 2113-2124 12 p.

Oligomers of α-synuclein: picking the culprit in the line-up

Lorenzen, N. & Otzen, D., 18 Aug 2014, In: Essays in Biochemistry. 56, 1, p. 137-148 12 p.

How epigallocatechin gallate can inhibit α-synuclein oligomer toxicity in vitro

Lorenzen, N., Nielsen, S. B., Yoshimura, Y., Vad, B. S., Andersen, C. B., Betzer, C., Kaspersen, J. D., Christiansen, G., Pedersen, J. S., Jensen, P. H., Mulder, F. A. A. & Otzen, D. E., 1 Aug 2014, In: Journal of Biological Chemistry. 289, 31, p. 21299-21310 12 p.

Co-existence of Two Different α-Synuclein Oligomers with Different Core Structures Determined by Hydrogen/Deuterium Exchange Mass Spectrometry

Paslawski, W., Mysling, S., Thomsen, K., Jørgensen, T. J. D. & Otzen, D., 14 Jul 2014, In: Angewandte Chemie International Edition. 53, 29, p. 7560-7563 4 p.

Bacterial RTX Toxins Allow Acute ATP Release from Human Erythrocytes Directly through the Toxin Pore

Skals, M., Bjaelde, R. G., Reinholdt, J., Poulsen, K., Vad, B. S., Otzen, D., Leipziger, J. & Praetorius, H. A., 4 Jul 2014, In: Journal of Biological Chemistry. 289, p. 19098-19109 11 p.

Condenser: A statistical aggregation tool for multi-sample quantitative proteomic data from Matrix Science Mascot Distiller™

Knudsen, A. D., Bennike, T., Kjeldal, H., Birkelund, S., Otzen, D. & Stensballe, A., 30 May 2014, In: Journal of Proteomics. 103, p. 261-266 6 p.

Folding of outer membrane protein A in the anionic biosurfactant rhamnolipid

Andersen, K. K. & Otzen, D., 21 May 2014, In: FEBS Letters. 588, 10, p. 1955-1960 6 p.

Comparison of two phenotypically distinct lattice corneal dystrophies caused by mutations in the transforming growth factor beta induced (TGFBI) gene

Poulsen, E. T., Runager, K., Risør, M. W., Dyrlund, T. F., Scavenius, C., Karring, H., Praetorius, J., Vorum, H., Otzen, D. E., Klintworth, G. K. & Enghild, J. J., Apr 2014, In: Proteomics - Clinical Applications. 8, 3-4, p. 168-177 10 p.

The role of stable α-synuclein oligomers in the molecular events underlying amyloid formation

Lorenzen, N., Nielsen, S. B., Buell, A. K., Kaspersen, J. D., Arosio, P., Vad, B. S., Paslawski, W., Christiansen, G., Valnickova Hansen, Z., Andreasen, M., Enghild, J. J., Pedersen, J. S., Dobson, C. M., Knowles, T. P. J. & Otzen, D., 16 Feb 2014, In: Journal of the American Chemical Society. 136, 10, p. 3859-3868 10 p.

Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation

Kronqvist, N., Otikovs, M., Chmyrov, V., Chen, G., Andersson, M., Nordling, K., Landreh, M., Sarr, M., Jörnvall, H., Wennmalm, S., Widengren, J., Meng, Q., Rising, A., Otzen, D., Knight, S. D., Jaudzems, K. & Johansson, J., 10 Feb 2014, In: Nature Communications. 5, 11 p., 3254.

The N-terminus of α-synuclein is essential for both monomeric and oligomeric interactions with membranes

Lorenzen, N., Lemminger, L., Pedersen, J. N., Nielsen, S. B. & Otzen, D., 31 Jan 2014, In: FEBS Letters. 588, 3, p. 497-502 6 p.

Electrochemical Analysis of the Fibrillation of Parkinson's Disease α-synuclein

Lopes, P., Dyrnesli, H., Lorenzen, N., Otzen, D. & Ferapontova, E., 2014, In: Analyst. 139, 4, p. 749-756 8 p.

Mutation in transforming growth factor beta induced protein associated with granular corneal dystrophy type 1 reduces the proteolytic susceptibility through local structural stabilization

Underhaug, J., Koldsø, H., Runager, K., Nielsen, J. T., Sørensen, C. S., Kristensen, T., Otzen, D., Karring, H., Malmendal, A., Schiøtt, B., Enghild, J. J. & Nielsen, N. C., 12 Oct 2013, In: BBA General Subjects. 1834, 12, p. 2812–2822 11 p.

Evolutionary Insight into the Functional Amyloids of the Pseudomonads

Dueholm, M. S., Otzen, D. & Nielsen, P. H., 7 Oct 2013, In: PLOS ONE. 8, 10, p. 1-9 9 p., e76630.

Formation of dynamic soluble surfactant-induced amyloid β peptide aggregation intermediates

Abelein, A., Kaspersen, J. D., Nielsen, S. B., Jensen, G. V., Christiansen, G., Pedersen, J. S., Danielsson, J. A., Otzen, D. & Gräslund, A., 9 Aug 2013, In: Journal of Biological Chemistry. 288, p. 23518-23528 11 p.

Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation

Dueholm, M. S., Søndergaard, M., Nilsson, M., Christiansen, G., Stensballe, A., Overgaard, M. T., Givskov, M. C., Tolker-Nielsen, T., Otzen, D. & Nielsen, P. H., Jun 2013, In: MicrobiologyOpen. 2, 3, p. 365-382 13 p.

Proliferation of amyloid-β42 aggregates occurs through a secondary nucleation mechanism

Cohen, S. I. A., Linse, S., Luheshi, L. M., Hellstrand, E., White, D. A., Rajah, L., Otzen, D., Vendruscolo, M., Dobson, C. M. & Knowles, T. P. J., 23 May 2013, In: PNAS (Proceedings of the National Academy of Sciences of the United States of America). 110, 24, p. 9758-9763 6 p.

Quality control system response to stochastic growth of amyloid fibrils

Pigolotti, S., Lizana, L., Otzen, D. & Sneppen, K., 2 May 2013, In: F E B S Letters. 587, 9, p. 1405-1410 6 p.

Novicidin's membrane permeabilizing activity is driven by membrane partitioning but not by helicity: A biophysical study of the impact of lipid charge and cholesterol

Balakrishnan, V. S., Vad, B. S. & Otzen, D., 2 Apr 2013, In: BBA General Subjects. 1834, 6, p. 996-1002 7 p.

Protein:fatty acid complexes: Biochemistry, biophysics and function

Brinkmann, C. R., Thiel, S. & Otzen, D., Apr 2013, In: F E B S Journal. 280, 8, p. 1733–1749

Off-pathway aggregation can inhibit fibrillation at high protein concentrations

Deva, T., Lorenzen, N., Vad, B. S., Petersen, S. V., Thørgersen, I., Enghild, J. J., Kristensen, T. & Otzen, D., Mar 2013, In: BBA General Subjects. 1834, 3, p. 677-687 11 p.

Variant of the Thermomyces lanuginosus lipase with improved kinetic stability: a candidate for enzyme replacement therapy

Wang, H., Hagedorn, J., Svendsen, A., Borch, K. & Otzen, D., Feb 2013, In: Biophysical Chemistry. 172, p. 43-52 10 p.

The neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins

Helwig, M., Hoshino, A., Berridge, C., Lee, S-N., Lorenzen, N., Otzen, D., Eriksen, J. & Lindberg, I., 11 Jan 2013, In: Journal of Biological Chemistry. 288, 2, p. 1114 1124 p.

pH regulation of the kinetic stability of the lipase from Thermomyces lanuginosus

Wang, H., Andersen, K. K., Sehgal, P., Hagedorn, J., Westh, P., Borch, K. & Otzen, D. E., 8 Jan 2013, In: Biochemistry. 52, 1, p. 264-276 13 p.

OmpA can form folded and unfolded oligomers

Wang, H., Andersen, K. K., Vad, B. S. & Otzen, D. E., 1 Jan 2013, In: B B A - Proteins and Proteomics. 1834, 1, p. 127-136 10 p.

Coexistence of ribbon and helical fibrils originating from hIAPP20-29 revealed by quantitative nanomechanical atomic force microscopy

Zhang, S., Andreasen, M., Nielsen, J. T., Liu, L., Nielsen, E. H., Song, J., Ji, G., Sun, F., Skrydstrup, T., Besenbacher, F., Nielsen, N. C., Otzen, D. E. & Dong, M., 2013, In: PNAS (Proceedings of the National Academy of Sciences of the United States of America). 110, 8, p. 2798-2803 6 p.

Folding of outer membrane proteins

Otzen, D. & Andersen, K. K., 2013, In: Archives of Biochemistry and Biophysics. 531, 1-2, p. 34-43 10 p.

Wildtype and A30P Mutant Alpha-Synuclein Form Different Fibril Structures

Nielsen, S. B., Macchi, F., Raccosta, S., Langkilde, A. E., Giehm, L., Kyrsting, A., Svane, A. S. P., Manno, M., Christiansen, G., Nielsen, N. C., Oddershede, L., Vestergaard, B. & Otzen, D., 2013, In: P L o S One. 8, 7, e67713.

Curli functional amyloid systems are phylogenetically widespread and display large diversity in operon and protein structure

Dueholm, M. S., Albertsen, M., Otzen, D. & Nielsen, P. H., 12 Dec 2012, In: P L o S One. 7, 12, p. e51274 18 p.

Eurocin, a new fungal defensin: structure, lipid binding and its mode of action

Oeemig, J. S., Lynggaard, C., Knudsen, D., Hansen, F. T., Noergaard, K. D., Schneider, T., Vad, B. S., Sandvang, D., Nielsen, L., Neve, S., Kristensen, H-H., Sahl, H-G., Otzen, D. & Wimmer, R., 7 Dec 2012, In: Journal of Biological Chemistry. 7, p. 42361-42372 11 p.

Polymorphic Fibrillation of the Destabilized Fourth Fasciclin-1 Domain Mutant A546T of the Transforming Growth Factor-β-induced Protein (TGFBIp) Occurs through Multiple Pathways with Different Oligomeric Intermediates

Andreasen, M., Nielsen, S. B., Runager, K., Christiansen, G., Nielsen, N. C., Enghild, J. J. & Otzen, D., 5 Oct 2012, In: Journal of Biological Chemistry. 287, 41, p. 34730-34742 12 p.

A Kinetic Analysis of the Folding and Unfolding of OmpA in Urea and Guaninidinium Chloride: Single and Parallel Pathways

Andersen, K. K., Wang, H. & Otzen, D., 12 Sept 2012, In: Biochemistry. 51, 42, p. 8371-8383 12 p.

Cyclodextrin-Scaffolded Alamethicin with Remarkably Efficient Membrane Permeabilizing Properties and Membrane Current Conductance

Hjørringgaard, C. U., Vad, B. S., Matchkov, V., Nielsen, S. B., Vosegaard, T., Nielsen, N. C., Otzen, D. & Skrydstrup, T., 5 Jul 2012, In: Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical. 116, 26, p. 7652-7659 8 p.

Proteolytic activation of proteose peptone component 3 by release of a C-terminal peptide with antibacterial properties

Pedersen, L. R. L., Hansted, J. G., Nielsen, S. B., Petersen, T. E., Sørensen, U. S., Otzen, D. & Sørensen, E. S., 1 Jun 2012, In: Journal of Dairy Science. 95, 6, p. 2819-2829 11 p.

Role of elongation and secondary pathways in S6 amyloid fibril growth

Lorenzen, N., Cohen, S. I. A., Nielsen, S. B., Herling, T. W., Christiansen, G., Dobson, C. M., T. P. Knowles, T. & Otzen, D., 2 May 2012, In: Biophysical Journal. 102, 9, p. 2167-2175 9 p.

Lactadherin binds to phosphatidylserine-containing vesicles in a two-step mechanism sensitive to vesicle size and composition

Otzen, D., Blans, K., Wang, H., Gilbert, G. E. & Rasmussen, J. T., Apr 2012, In: BBA General Subjects. 1818, 4, p. 1019-1027 9 p.

The effect of osmolytes on protein fibrillation

Macchi, F., Eisenkolb, M., Kiefer, H. & Otzen, D., 21 Mar 2012, In: International Journal of Molecular Sciences (Online). 13, 3, p. 3801-3819 19 p.

Mapping out the multistage fibrillation of glucagon

Ghodke, S., Nielsen, S. B., Christiansen, G., Hjuler, H. A., Flink, J., Otzen, D. & Nielsen, S. B., Mar 2012, In: F E B S Journal. 279, 5, p. 752-765 13 p.

A Ser residue influences the structure and stability of a Pro-kinked transmembrane helix dimer

Weber, M., Tome, L., Otzen, D. & Schneider, D., 2012, In: BBA General Subjects. 1818, 9, p. 2103-7 5 p.

Mapping of unfolding states of integral helical membrane proteins by GPS-NMR and scattering techniques: TFE-induced unfolding of KcsA in DDM surfactant

Calcutta, A., Jessen, C. M., Behrens, M. A., Oliveira, C. L. P., Renart, M. L., González-Ros, J. M., Otzen, D., Pedersen, J. S., Malmendal, A. & Nielsen, N. C., 2012, In: B B A - Biomembranes. 1818, 9, p. 2290-301 12 p.

Modulation of fibrillation of hIAPP core fragments by chemical modification of the peptide backbone

Andreasen, M., Nielsen, S. B., Mittag, T., Bjerring, M., Nielsen, J. T., Zhang, S., Nielsen, E. H., Jeppesen, M., Christiansen, G., Besenbacher, F., Dong, M., Nielsen, N. C., Skrydstrup, T. & Otzen, D. E., 2012, In: B B A - Proteins and Proteomics. 1824, 2, p. 274-85 12 p.

Multiple Roles of Heparin in the Aggregation of p25α

Nielsen, S. B., Yde, P., Giehm, L., Sundbye, S., Christiansen, G., Matthiesen, J., Jensen, M. H., Jensen, P. H. & Otzen, D. E., 2012, In: Journal of Molecular Biology.

N for AsN - O for StrOcture? A strand-loop-strand motif for prokaryotic O-glycosylation

Otzen, D., 2012, In: Molecular Oral Microbiology.

PP3 forms stable tetrameric structures through hydrophobic interactions via the C-terminal amphipathic helix and undergoes reversible thermal dissociation and denaturation

Pedersen, L. R. L., Nielsen, S. B., Hansted, J. G., Petersen, T. E., Otzen, D. E. & Sørensen, E. S., 2012, In: F E B S Journal. 279, 2, p. 336-47 12 p.

Scanning tunneling microscopy reveals single-molecule insights into the self-assembly of amyloid fibrils

Kalashnyk, N., Nielsen, J. T., Nielsen, E. H., Skrydstrup, T., Otzen, D., Lægsgaard, E., Wang, C., Besenbacher, F., Nielsen, N. C. & Linderoth, T. R., 2012, In: A C S Nano. 6, 8, p. 6882-6889 8 p.

1-16 Can Aggregate and induce the Production of Reactive Oxygen Species, Nitric Oxide, and Inflammatory Cytokines

Du, X., Wang, L., Wang, Y., Andreasen, M., Zhan, D., Feng, Y., Li, M., Zhao, M., Otzen, D., Xue, D., Yang, Y. & Liu, R., Nov 2011, In: Journal of Alzheimer's Disease. 27, 2, p. 401-413 12 p.

Assembling good amyloid some structures at last

Otzen, D. E., 7 Sept 2011, In: Structure. 19, 9, p. 1207-1209 3 p.

Hydrogen exchange mass spectrometry as an analytical tool for the analysis of amyloid fibrillogenesis

Scavenius, C., Ghodke, S., Otzen, D. E. & Enghild, J. J., 30 Apr 2011, In: International Journal of Mass Spectrometry. 302, 1-3, p. 167-173 7 p.

Low-resolution structure of a vesicle disrupting α-synuclein oligomer that accumulates during fibrillation

Giehm, L., Svergun, D. I., Otzen, D. E. & Vestergaard, B., 22 Feb 2011, In: Proceedings of the National Academy of Sciences. 108, 8, p. 3246-3251 6 p.

Human Phenotypically Distinct TGFBI Corneal Dystrophies Are Linked to the Stability of the Fourth FAS1 Domain of TGFBIp

Runager, K., Basaiawmoit, R. V., Deva, T., Andreasen, M., Valnickova, Z., Sørensen, C. S., Karring, H., Thøgersen, I. B., Christiansen, G., Underhaug, J., Kristensen, T., Nielsen, N. C., Klintworth, G. K., Otzen, D. E. & Enghild, J. J., 18 Feb 2011, In: Journal of Biological Chemistry. 286, 7, p. 4951-8 8 p.

Mapping the folding pathway of the transmembrane protein DsbB by protein engineering

Otzen, D. E., 1 Jan 2011, In: Protein Engineering Design and Selection (Print). 24, 1-2, p. 139-149 11 p.

The role of membrane properties in Mistic folding and dimerisation

Debnath, D. K., Basaiawmoit, R. V., Nielsen, K. L. & Otzen, D. E., 1 Jan 2011, In: Protein Engineering Design and Selection (Print). 24, 1-2, p. 89-97 9 p.

Assays for alpha-synuclein aggregation

Giehm, L., Lorenzen, N. & Otzen, D., 2011, In: Methods. 53, 3, p. 295-305 11 p.

Effect of protein-surfactant interactions on aggregation of β-lactoglobulin

Hansted, J. G., Wejse, P. L., Bertelsen, H. & Otzen, D. E., 2011, In: BBA General Subjects. 1814, 5, p. 713-723 11 p.

Fibrillation of the major curli subunit CsgA under a wide range of conditions implies a robust design of aggregation

Dueholm, M. S., Nielsen, S. B., Hein, K. L., Nissen, P., Chapman, M., Christiansen, G., Nielsen, P. H. & Otzen, D. E., 2011, In: Biochemistry. 50, 39, p. 8281-90 10 p.

Long-term-stable ether-lipid vs conventional ester-lipid bicelles in oriented solid-state NMR: altered structural information in studies of antimicrobial peptides

Bertelsen, K., Vad, B., Nielsen, E. H. T., Hansen, S. K., Skrydstrup, T., Otzen, D., Vosegaard, T. & Nielsen, N. C., 2011, In: Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical. 115, 8, p. 1767-1774 8 p.

Mechanical stress affects glucagon fibrillation kinetics and fibril structure

Macchi, F., Hoffmann, S. V., Carlsen, M., Vad, B. S., Imparato, A., Rischel, C. & Otzen, D., 2011, In: Langmuir. 27, 20, p. 12539–12549

Protein-surfactant interactions a tale of many states

Otzen, D., 2011, In: BBA General Subjects. 1814, 5, p. 562-91 30 p.

SAXS models of TGFBIp reveal a trimeric structure and show that the overall shape is not affected by the Arg124His mutation

Basaiawmoit, R. V., Oliveira, C. L. P., Runager, K., Sørensen, C. S., Behrens, M. A., Jonsson, B-H., Kristensen, T., Klintworth, G. K., Enghild, J. J., Pedersen, J. S. & Otzen, D. E., 2011, In: Journal of Molecular Biology. 408, 3, p. 503-513 11 p.

SDS-facilitated in vitro formation of a transmembrane B-type cytochrome is mediated by changes in local pH

Weber, M., Schneider, D., Prodöhl, A., Dreher, C., Becker, C., Underhaug, J., Svane, A. S. P., Malmendal, A., Nielsen, N. C. & Otzen, D., 2011, In: Journal of Molecular Biology. 407, 4, p. 594-606 13 p.

Soft Interactions at Nanoparticles Alter Protein Function and Conformation in a Size Dependent Manner

Wang, J., Jensen, U. B., Jensen, G. V., Shipovskov, S., Balakrishnan, V. S., Otzen, D., Pedersen, J. S., Besenbacher, F. & Sutherland, D. S., 2011, In: Nano Letters. 11, 11, p. 4985-4991 6 p.

Assays for α-synuclein aggregation

Giehm, L., Lorenzen, N. & Otzen, D. E., 14 Dec 2010, In: Methods.

Human phenotypically distinct TGFI corneal dystrophies are linked to the stability of the fourth Fas1 domain of TGFBIp

Otzen, D. & Enghild, J. J., 6 Dec 2010, In: Journal of Biological Chemistry.

Strategies to increase the reproducibility of α-synuclein fibrillation in plate reader assays

Giehm, L. & Otzen, D., 15 Nov 2010, In: Analytical Biochemistry. 406, 2, p. 270-281 12 p.

Cell-free synthesis and folding of transmembrane OmpA reveals higher order structures and premature truncations

Debnath, D. K. & Otzen, D. E., 1 Nov 2010, In: Advances in Biophysical Chemistry. 152, 1-3, p. 80-8 9 p.

Amyloid structure--one but not the same: the many levels of fibrillar polymorphism

Pedersen, J. S., Andersen, C. B. & Otzen, D., Nov 2010, In: The F E B S Journal (Online). 277, 22, p. 4591-4601 11 p.

An Aß concatemer with altered aggregation propensities

Giehm, L., Dal Degan, F., Fraser, P., Klysner, S. & Otzen, D. E., 1 Oct 2010, In: BBA General Subjects. 1804, 10, p. 2025-35 11 p.

Functional amyloid: turning swords into plowshares

Otzen, D., 1 Oct 2010, In: Prion. 4, 4, p. 256-64 9 p.

Cyclodextrin scaffolded alamethicin with highly efficient channel-forming properties

Hjørringgaard, C. U., Vad, B., Nielsen, S. B., Matchkov, V., Vosegaard, T., Nielsen, N. C., Otzen, D. & Skrydstrup, T., Sept 2010, In: Journal of Peptide Science. 16, p. 148 1 p.

Amyloid formation in surfactants and alcohols: membrane mimetics or structural switchers?

Otzen, D. E., 1 Aug 2010, In: Current protein & peptide science. 11, 5, p. 355-71 17 p.

beta-Sheet aggregation of kisspeptin-10 is stimulated by heparin but inhibited by amphiphiles

Nielsen, S. B., Franzmann, M., Basaiawmoit, R. V., Wimmer, R., Mikkelsen, J. D. & Otzen, D. E., 1 Aug 2010, In: Biopolymers. 93, 8, p. 678-89 12 p.

Stop-and-go kinetics in amyloid fibrillation

Ferkinghoff-Borg, J., Fonslet, J., Andersen, C. B., Krishna, S., Pigolotti, S., Yagi, H., Goto, Y., Otzen, D. & Jensen, M. H., 1 Jul 2010, In: Physical Review E. Statistical, Nonlinear, and Soft Matter Physics. 82, 1 Pt 1, p. 010901

The diversity of FtsY-lipid interactions

Reinau, M. E., Thøgersen, I. B., Enghild, J. J., Nielsen, K. L. & Otzen, D. E., 1 Jul 2010, In: Biopolymers. 93, 7, p. 595-606 12 p.

Occupancy of nonannular lipid binding sites on KcsA greatly increases the stability of the tetrameric protein

Triano, I., Barrera, F. N., Renart, M. L., Molina, M. L., Fernández-Ballester, G., Poveda, J. A., Fernández, A. M., Encinar, J. A., Ferrer-Montiel, A. V., Otzen, D. & González-Ros, J. M., 29 Jun 2010, In: Biochemistry. 49, 25, p. 5397-5404 8 p.

A thermodynamic analysis of fibrillar polymorphism

Jeppesen, M. D., Hein, K., Nissen, P., Westh, P. & Otzen, D. E., 1 Jun 2010, In: Advances in Biophysical Chemistry. 149, 1-2, p. 40-6 7 p.

Strategies to increase the reproducibility of protein fibrillization in plate reader assays

Giehm, L. & Otzen, D. E., 15 May 2010, In: Analytical Biochemistry. 400, 2, p. 270-81 12 p.

In vitro association of fragments of a beta-sheet membrane protein

Debnath, D., Nielsen, K. L. & Otzen, D. E., 1 May 2010, In: Advances in Biophysical Chemistry. 148, 1-3, p. 112-20 9 p.

Fast mapping of global protein folding states by multivariate NMR: a GPS for proteins

Malmendal, A., Underhaug, J., Otzen, D. E. & Nielsen, N. C., 21 Apr 2010, In: P L o S One. 5, 4, p. e10262 6 p.

The role of protonation in protein fibrillation

Jeppesen, M. D., Westh, P. & Otzen, D. E., 19 Feb 2010, In: FEBS Letters. 584, 4, p. 780-4 5 p.

Pardaxin permeabilizes vesicles more efficiently by pore formation than by disruption

Vad, B. S., Bertelsen, K., Johansen, C. H., Pedersen, J. M., Skrydstrup, T., Nielsen, N. C. & Otzen, D. E., 17 Feb 2010, In: Biophysical Journal. 98, 4, p. 576-85 10 p.

Impact of the antimicrobial peptide Novicidin on membrane structure and integrity

Nielsen, S. B. & Otzen, D. E., 28 Jan 2010, In: Journal of Colloid and Interface Science. 345, 2, p. 248-256 9 p.

Crystallographic analysis reveals a unique lidocaine binding site on human serum albumin

Hein, K. L., Kragh-Hansen, U., Morth, J. P., Jeppesen, M., Otzen, D., Møller, J. V. & Nissen, P., 2010, In: Journal of Structural Biology. 171, p. 353-360

Divorcing folding from function: how acylation affects the membrane-perturbing properties of an antimicrobial peptide

Vad, B. S., Thomsen, L. A. H., Bertelsen, K., Franzmann, M., Pedersen, J. M., Nielsen, S. B., Vosegaard, T., Valnickova, Z., Skrydstrup, T., Enghild, J. J., Wimmer, R., Nielsen, N. C. & Otzen, D., 2010, In: B B A - Proteins and Proteomics. 1804, 4, p. 806-20 15 p.

Flexibility of the thrombin-activatable fibrinolysis inhibitor pro-domain enables productive binding of protein substrates

Valnickova, Z., Sanglas, L., Arolas, J. L., Petersen, S. V., Schar, C., Otzen, D., Aviles, F. X., Gomis-Ruth, F. X. & Enghild, J. J., 2010, In: Journal of Biological Chemistry.

Functional amyloid in Pseudomonas

Dueholm, M. S., Petersen, S. V., Sønderkær, M., Larsen, P., Christiansen, G., Hein, K. L., Enghild, J. J., Nielsen, J. L., Nielsen, K. L., Nielsen, P. H. & Otzen, D. E., 2010, In: Molecular Microbiology.

Functional bacterial amyloids in biofilms

Otzen, D., 2010, In: Annual Biofilm Highlights.

Glucagon fibril polymorphism reflects differences in protofilament backbone structure

Andersen, C. B., Hicks, M. R., Vetri, V., Vandahl, B., Rahbek-Nielsen, H., Thøgersen, H., Thøgersen, I., Enghild, J. J., Serpell, L. C., Rischel, C. & Otzen, D. E., 2010, In: Journal of Molecular Biology. 397, 4, p. 932-46 14 p.

Institutional nonconformism: the many levels of glucagon polymorphism

Otzen, D., 2010, In: F E B S Journal.

Membrane Interactions of Novicidin, a Novel Antimicrobial Peptide: Phosphatidylglycerol Promotes Bilayer Insertion

Dorosz, J., Gofman, Y., Kolusheva, S., Otzen, D., Ben-Tal, N., Nielsen, N. C. & Jelinek, R., 2010, In: The Journal of Physical Chemistry B. 114, 34, p. 11053-60 8 p.

Occupancy of non-annular lipid binding sites on KcsA greatly increases the stability of the tetrameric protein

Otzen, D., 2010, In: Biochemistry.

SDS-Induced Fibrillation of α-Synuclein: An Alternative Fibrillation Pathway

Giehm, L., Oliveira, C. L. P. D., Christiansen, G., Pedersen, J. S. & Otzen, D., 2010, In: Journal of Molecular Biology. 401, p. 115-133

Surfactants and alcohols as inducers of protein amyloid: aggregation chaperones or membrane simulators?

Otzen, D., 2010, In: Lipids and membranes in amyloid diseases.

The interaction of equine lysozyme:oleic acid complexes with lipid membranes suggests a cargo off-loading mechanism

Nielsen, S. B., Wilhelm, K., Vad, B., Schleucher, J., Morozova-Roche, L. A. & Otzen, D., 2010, In: Journal of Molecular Biology. 398, 2, p. 351-61 11 p.

Synthesis of a Ketomethylene Isostere of the Fibrillating Peptide SNNFGAILSS

Mittag, T., Nielsen, N. C., Otzen, D. & Skrydstrup, T., 9 Sept 2009, In: Journal of Organic Chemistry. 74, p. 7955-7957

A Versatile Approach to β-Amyloid Fibril-Binding Compounds Exploiting the Shirakawa/Hayashi Protocol for trans-Alkene Synthesis

Huynh, T. H. V., Mantel, M., Mikkelsen, K., Lindhardt, A. T., Nielsen, N. C., Otzen, D. & Skrydstrup, T., 22 Jan 2009, In: Organic Letters. 11, p. 999-1002

Branching in amyloid fibril growth

Beyschau Andersen, C., Yagi, H., Manno, M., Martorana, V., Ban, T., Christiansen, G., Otzen, D., Goto, Y. & Rischel, C., 2009, In: Biophysical Journal. 96, 4, p. 1529-36 7 p.

How chain length and charge affect surfactant denaturation of ACBP

Andersen, K. & Otzen, D., 2009, In: Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical. 113, p. 13942-13952

Influence of tunable external stimuli on the self-assembly of guanosine supramolecular nanostructures studied by atomic force microscope

Li, Y., Dong, M., Otzen, D., Yao, Y., Liu, B., Besenbacher, F. & Mamdouh, W., 2009, In: Langmuir. 25

Interaction and stability of mixed micelle and monolayer of nonionic and cationic surfactant mixtures

Sehgal, P., Kosaka, O., Doe, H. & Otzen, D., 2009, In: Journal of Dispersion Science and Technology. 30, p. 1050-1058

Interaction of a potyviral VPg with anionic phospholipid vesicles

Rantalainen, K., Christensen, P. A., Hafrén, A., Otzen, D., Kalkkinen, N. & Mäkinen, K., 2009, In: Virology. 395, p. 114-120

Interactions and Influence of a-Cyclodextrin on the Aggregation and Interfacial Properties of Mixtures of Nonionic and Zwitterionic Surfactants

Sehgal, P., Mizuki, T., Doe, H., Wimmer, R., Larsen, K. L. & Otzen, D., 2009, In: Colloid and Polymer Science. 287, p. 1243-1252

Kinetic partitioning between aggregation and vesicle permeabilization by modified ADan

Nesgaard, L. W., Vad, B., Christiansen, G. & Otzen, D., 2009, In: B B A - Molecular Cell Research. 1794, p. 84-93

Proteasome-Fibrillation Antagonism in a Dynamic Model for Parkinson’s Disease

Sneppen, K., Lizana, G. L., Jensen, M. H., Pigolotti, S. & Otzen, D., 2009, In: Physical Biology. 6, p. 36005

Proteomic changes in response to chromium(VI) toxicity in Pseudomonas aeruginosa

Kilic, N., Stensballe, A., Otzen, D. & Dönmez, G., 2009, In: Bioresource Technology. 101, p. 2134-2140

Quantitative use of paramagnetic relaxation enhancements for determining orientation and insertion depth of peptides in micelles

Franzmann, M., Otzen, D. & Wimmer, R., 2009, In: ChemBioChem. 10, p. 2339-2347

SAXS Study of Glucagon Fibrillation: From Monomers to Fibers

Oliveira, C. L. P. D., Behrens, M. A., Pedersen, J. S., Erlacher, K., Otzen, D. & Pedersen, J. S., 2009, In: Journal of Molecular Biology. 387, p. 147-161

Stability and structure of the membrane protein transporter Ffh is modulated by substrates and lipids

Reinau, M. E. & Otzen, D., 2009, In: Archives of Biochemistry and Biophysics. 492, p. 48-53

Stable intermediates determine proteins' primary unfolding sites in the presence of surfactants

Hansen, J. H., Petersen, S. V., Andersen, K. K., Enghild, J. J., Damhus, T. & Otzen, D., 2009, In: Biopolymers. 91, 3, p. 221-31 10 p.

Surfactant protein SP-B strongly modifies surface collapse of phospholipid vesicles: Insights from a quartz crystal microbalance with dissipation

Cabré, E., Malmström, J., Sutherland, D., Perez-Gil, J. & Otzen, D., 2009, In: Biophysical Journal. 97, 3, p. 768-76 8 p.

The influence of vesicle composition and size on a-synuclein structure and stability

Kjær, L., Giehm, L., Heimburg, T. R. & Otzen, D., 2009, In: Biophysical Journal. 96, 2857 p.

The role of decorated SDS micelles in sub-cmc protein denaturation and association

Andersen, K., Oliveira, C. L. P. D., Larsen, K. L., Poulsen, F., Callisen, T., Westh, P., Pedersen, J. S. & Otzen, D., 2009, In: Journal of Molecular Biology. 391, p. 207-226

Unique identification of supramolecular structures in amyloid fibrils by solid-state NMR spectroscopy

Nielsen, J. T., Bjerring, M., Jeppesen, M., Pedersen, R. O., Pedersen, J. M., Hein, K. L., Vosegaard, T., Skrydstrup, T., Otzen, D. & Nielsen, N. C., 2009, In: Angewandte Chemie International Edition. 48, p. 2118-2121 4 p.

Widespread abundance of Functional Bacterial Amyloid in Mycolata and other Gram positive Bacteria

Jensen, P. L., Dueholm, M. S., Larsen, P., Petersen, S. V., Enghild, J. J., Christiansen, G., Højrup, P., Nielsen, P. H. & Otzen, D., 2009, In: Applied and Environmental Microbiology. 75, p. 4101-4110

α-lactalbumin is unfolded by all classes of detergents but with different mechanisms

Otzen, D., Sehgal, P. & Westh, P., 2009, In: Journal of Colloid and Interface Science. 329, p. 273-283

A global study of myoglobin-surfactant interactions

Andersen, K. K., Westh, P. & Otzen, D., 2008, In: Langmuir. 15, p. 399-407

AFM-based force spectroscopy measurements of mature amyloid fibrils of the peptide glucagon: Nanotechnology

Dong, M. D., Hovgaard, M. B., Mamdouh, W., Xu, S. L., Otzen, D. E. & Besenbacher, F., 2008, In: Nanotechnology. 19, 38

AFM-based force spectroscopy measurements of mature amyloid fibrils of the peptide glucagon

Dong, M., Hovgaard, M. B., Mamdouh, W., Xu, S., Otzen, D. & Besenbacher, F., 2008, In: Nanotechnology. 19, p. 384013 7 p.

Aggregation of S6 in a quasi-native state by monomeric SDS

Otzen, D., Nesgaard, L., Andersen, K. K., Hansen, J. H., Christiansen, G., Doe, H. & Sehgal, P., 2008, In: B B A - Biomembranes. 1784, p. 400-414

Amyloid - a state in many guises: survival of the fittest fibril fold

Pedersen, J. S. & Otzen, D., 2008, In: Protein Science. 17, p. 1-9

Amyloid-like adhesins in floc-forming and filamentous bacteria in activated sludge

Larsen, P., Nielsen, J. L. & Otzen, D., 2008, In: Applied and Environmental Microbiology. 74, p. 1517-1526

An unusual intrinsically disordered protein from the model legume Lotus japonicus stabilizes proteins in vitro

Haaning, S., Radutoiu, S., Hoffmann, S. V., Dittmer, J., Giehm, L., Otzen, D. & Stougaard, J., 2008, In: Journal of Biological Chemistry. 283, 45, p. 31142-52 10 p.

Characterization of Dry Globular Proteins and Protein Fibrils by Synchrotron Radiation Vacuum UV Circular Dichroism

Nesgaard, L. W., Hoffmann, S. V., Andersen, C. B., Malmendal, A. & Otzen, D., 2008, In: Biopolymers. 89, 9, p. 779-795

Dendrimers destabilize proteins in a generation-dependent manner involving eletrostatic interactions

Giehm, L., Christensen, C., Boas, U., Heegaard, P. M. H. & Otzen, D., 2008, In: Biopolymers. 89, p. 522-529

Differential adsorption of variants of the thermomyces lanuginosus lipase on a hydrophobic surface suggests a role for local flexibility

Otzen, D., 2008, In: Colloids and Surfaces B: Biointerfaces. 64, p. 223-228

Early Stages of Amyloid Fibril Formation Studied by Liquid-State NMR: The Peptide Hormone Glucagon

Svane, A. S. P., Jahn, K., Deva, T., Malmendal, A., Otzen, D., Dittmer, J. & Nielsen, N. C., 2008, In: Biophysical Journal. 95, 1, p. 366 – 377 11 p.

Effect of glycosylation on biphysical and flocculative properties of the extracellular domain of Ag43

Knudsen, S. K., Westergaard, U. B., Frantzmann, M., Stensballe, A. & Otzen, D., 2008, In: Biochemical Journal. 412, p. 563-577

Influence of beta-Cyclodextrin on the Mixed Micellization Process of Sodium Dodecyl Sulfate and Sodium Lauroyl Sarcosine and Formation of Inclusion Complexes

Sehgal, P., Sharma, D., Larsen, K. L., Wimmer, R., Otzen, D. & Doe, H., 2008, In: Journal of Dispersion Science and Technology. 29, p. 128-133

Interactions of g-Cyclodextrin with the Mixed Micelles of Anionic Surfactants and Their Inclusion Complexes Formation

Sehgal, P., Sharma, M., Larsen, K. L., Wimmer, R., Doe, H. & Otzen, D., 2008, In: Journal of Dispersion Science and Technology. 29, p. 885-890

Interactions of γ-Cyclodextrin with the Mixed Micelles of Anionic Surfactants and Their Inclusion Complexes Formation

Sehgal, P., Sharma, M., Larsen, K. L., Wimmer, R., Doe, H. & Otzen, D., 2008, In: Journal of Dispersion Science and Technology. 29, p. 885-890

Spectroscopic Evidence for the Existence of an Obligate Pre-Fibrillar Oligonomer during Glucagon Fibrillation

Christensen, P. A., Pedersen, J. S., Christiansen, G. & Otzen, D., 2008, In: F E B S Letters. 582, p. 1341-1345

Thermodynamics and mechanism of cutinase stabilization by trehalose

Baptista, R. P., Pedersen, S. H., Cabrita, G. J. M., Otzen, D., Cabral, J. M. & Melo, E. P., 2008, In: Biopolymers. 89, p. 538-547

We find them here, we find them there: Functional bacterial amyloid

Otzen, D. & Nielsen, P. H., 2008, In: Cellular and Molecular Life Sciences. 65, p. 910-927

p25α relocalizes in oligodendroglia from myelin to cytoplasmic inclusions in multiple system atrophy

Song, Y. J. C., Lundvig, D. M. S., Huang, Y., Wei, P. G., Blumbergs, P. C., Højrup, P., Otzen, D., Halliday, G. M. & Jensen, P. H., Oct 2007, In: American Journal of Pathology. 171, 4, p. 1291-1303 13 p.

Heparin binding induces a conformational change in pigment epithelium-derived factor

Valnickova, Z., Petersen, S. V., Nielsen, S. B., Otzen, D. E. & Enghild, J. J., 2 Mar 2007, In: Journal of Biological Chemistry. 282, 9, p. 6661-6667 7 p.

Alternative membrane protein conformations in alcohol

Otzen, D., Sehgal, P. & Nesgaard, L., 2007, In: Biochemistry. 46, p. 4348-4359

Amyloid adhesins are abundant in natural biofilms

Larsen, P., Dueholm, M. S., Christiansen, G., Nielsen, J. L., Otzen, D. & Nielsen, P. H., 2007, In: Env. Microbiol.. 9, p. 3077-3090

Changes in structures of milk proteins upon photo-oxidation

Dalsgaard, T. K., Otzen, D., Nielsen, J. H. & Larsen, L. B., 2007, In: J. Agr. Food Chem.. 55, p. 10968-10976

Dendrimer effects on peptide and protein fibrillation

Heegaard, P. M. P., Boas, U. & Otzen, D., 2007, In: Macromolecular Bioscience. 7, p. 1047-1059

Heparin binding induces a conformational change of pigment epithelium-derived factor (PEDF).

Valnickova, Z., Petersen, S. V., Nielsen, SB., Otzen, D. & Enghild, J. J., 2007, In: Journal of Biological Chemistry.

Modulation of cutinase structure and stability by phospholipid detergents

Sehgal, P., Bang Nielsen, S., Pedersen, S., Wimmer, R. & Otzen, D., 2007, In: Biochim. Biophys. Acta.. 1774, p. 1544-1554

Quartz crystal microbalance studies of multilayer glucagon fibrillation at the solid-liquid interface

Hovgaard, M. B., Dong, M., Otzen, D. E. & Besenbacher, F., 2007, In: Biophysical Journal. 93, 6, p. 2162-9 7 p.

Quartz crystal microbalance studies of multilayer glucagons fibrillation at the solid-liquid interface

Hovgaard, M. B., Dong, M., Otzen, D. & Besenbacher, F., 2007, In: Biophysical Journal. 93, 6, p. 2162

The Major Allergen from Birch Tree Pollen, Bet v 1, Binds and Permeabilizes Membranes.

Morgensen, JE., Ferreras, M., Wimmer, R., Petersen, S. V., Enghild, J. J. & Otzen, D., 2007, In: Biochemistry.

Unfolding of ß-sheet proteins in SDS

Nielsen, M. M., Andersen, K. K., Westh, P. & Otzen, D., 2007, In: Biophysical Journal. 92, p. 3674-3685

We find them here, we find them there: Functional bacterial amyloid

Otzen, D. & Nielsen, P. H., 2007, In: Cell. Mol. Life Sci..

p25a Relocalizes in Oligodendroglia from Myelin to Cytoplasmic Inclusions in Multiple System Atrophy

Song, Y. J. C., Lundvig, D. M. S., Huang, Y., Gai, W. P., Blumbergs, P. C., Højrup, P., Otzen, D., Halliday, G. M. & Jensen, P. H., 2007, In: Am. J. Path.. 171, p. 1291-1303

Interactions between anionic mixed micelles and α-cyclodextrin and their inclusion complexes: conductivity, NMR and fluorescence study

Sehgal, P., Sharma, M., Wimmer, R., Larsen, K. L. & Otzen, D., 2006, In: Colloid and Polymer Science. 284, 8, p. 916-926

N- and C-Terminal Hydrophobic Patches Are Involved in Fibrillation of Glucagon

Pedersen, J. S., Dikov, D. & Otzen, D., 2006, In: Biochemistry. 45, 48, p. 14503-14512 10 p.

Sulfates Dramatically Stabilize a Salt-Dependent Type of Glucagon Fibrils

Pedersen, J. S., Flink, J. M., Dikov, D. & Otzen, D., 2006, In: Biophysical Journal. 90, 11, p. 4181-4194

Thermodynamics of unfolding of an integral membrane protein in mixed micelles

Sehgal, P. & Otzen, D., 2006, In: Protein Science. 15, p. 890-899

Antagonism, non-native interactions and non-two-state folding in S6 revealed by double-mutant cycle analysis

Otzen, D., 1 Nov 2005, In: Protein Engineering, Design and Selection. 18, 11, p. 547-557 11 p.

Synergistic behavior of sodiumdodecylsulfate and 1,2-diheptanoyl-sn- glycero-3-phosphocholine in an aqueous medium: Interfacial and bulk behavior

Sehgal, P., Doe, H., Sharma, M. & Otzen, D. E., 1 Aug 2005, In: Colloid and Polymer Science. 283, 11, p. 1219-1225 7 p.

Interactions between folding factors and bacterial outer membrane proteins

Mogensen, J. E. & Otzen, D. E., 1 Jul 2005, In: Molecular Microbiology. 57, 2, p. 326-346 21 p.

Conformational detours during folding of a collapsed state

Otzen, D. E., 30 Jun 2005, In: Biochimica et Biophysica Acta - Proteins and Proteomics. 1750, 2, p. 146-153 8 p.

Activation, Inhibition, and Destabilization of Thermomyces lanuginosus Lipase by Detergents

Mogensen, J. E., Sehgal, P. & Otzen, D., 2005, In: Biochemistry. 44, 5, p. 1719-1730

Barriers to Folding of the Transmembrane Domain of the Escherichia coli Autotransporter Adhesin Involved in Diffuse Adherence

Mogensen, J. E., Tapadar, D., Schmidt, M. A. & Otzen, D., 2005, In: Biochemistry. 44, p. 4533-4545

Misfolding of a bacterial autotransporter

Mogensen, J. E., Kleinschmidt, J. H., Schmidt, M. A. & Otzen, D., 2005, In: Protein Science. 14, p. 2814-2827

Protein drug stability: a formulation challenge

Frøkjær, S. & Otzen, D., 2005, In: Nature Reviews. Drug Discovery. 4, p. 298-306

Stabilization of the Ribosomal Protein S6 by Trehalose is Counterbalanced by the Formation of a Putative Off-pathway Species

Chen, L., Cabrita, G. J. M., Otzen, D. & Melo, E. P., 2005, In: Journal of Molecular Biology. 351, p. 402-416

Using micellar mole fractions to assess membrane protein stability in mixed micelles

Sehgal, P., Mogensen, J. E. & Otzen, D., 2005, In: B B A - Biomembranes. 1761, p. 59-68 10 p.

Correspondence between anomalous m- and ΔCp-values in protein folding

Otzen, D. E. & Oliveberg, M., 1 Dec 2004, In: Protein Science. 13, 12, p. 3253-3263 11 p.

Investigating porcine pancreatic phospholipase A 2 action on vesicles and supported planar bilayers using a quartz crystal microbalance with dissipation

Justesen, P. H., Kristensen, T., Ebdrup, T. & Otzen, D., 15 Nov 2004, In: Journal of Colloid and Interface Science. 279, 2, p. 399-409 11 p.

Transient aggregation and stable dimerization induced by introducing an Alzheimer sequence into a water-soluble protein

Otzen, D. E., Miron, S., Akke, M. & Oliveberg, M., 19 Oct 2004, In: Biochemistry. 43, 41, p. 12964-12978 15 p.

Modulation of S6 fibrillation by unfolding rates and gatekeeper residues

Pedersen, J. S., Christensen, G. & Otzen, D. E., 6 Aug 2004, In: Journal of Molecular Biology. 341, 2, p. 575-588 14 p.

Interaction between phospholipid vesicular structures with long chain zwitterionic surfactants

Sehgal, P., Doe, H., Sharma, M. & Otzen, D. E., 1 May 2004, In: Colloid and Polymer Science. 282, 7, p. 677-683 7 p.

Transient formation of nano-crystalline structures during fibrillation of an Aβ-like peptide

Otzen, D. E. & Oliveberg, M., 1 May 2004, In: Protein Science. 13, 5, p. 1417-1421 5 p.

Elimination of A Misfolded Folding Intermediate by A Single Point Mutation

Mogensen, J. E., Ipsen, H., Holm, J. & Otzen, D. E., 30 Mar 2004, In: Biochemistry. 43, 12, p. 3357-3367 11 p.

Structural background of cyclodextrin-protein interactions

Aachmann, F. L., Otzen, D. E., Larsen, K. L. & Wimmer, R., 1 Dec 2003, In: Protein Engineering. 16, 12, p. 905-912 8 p.

Trehalose favors a cutinase compact intermediate off-folding pathway

Melo, E. P., Chen, L. Y., Cabral, J. M. S., Fojan, P., Petersen, S. B. & Otzen, D. E., 24 Jun 2003, In: Biochemistry. 42, 24, p. 7611-7617 7 p.

Adsorption of a small protein to a methyl-terminated hydrophobic surfaces: Effect of protein-folding thermodynamics and kinetics

Otzen, D. E., Oliveberg, M. & Höök, F., 15 May 2003, In: Colloids and Surfaces B: Biointerfaces. 29, 1, p. 67-73 7 p.

Folding of DsbB in mixed micelles: a kinetic analysis of the stability of a bacterial membrane protein.

Otzen, D. E., 1 Jan 2003, In: Journal of Molecular Biology. 330, 4, p. 641-649 9 p.

Protein unfolding in detergents: Effect of micelle structure, ionic strength, pH, and temperature

Otzen, D. E., 1 Oct 2002, In: Biophysical Journal. 83, 4, p. 2219-2230 12 p.

Structural basis for cyclodextrins' suppression of human growth hormone aggregation

Otzen, D. E., Knudsen, B. R., Aachmann, F., Larsen, K. L. & Wimmer, R., 2 Jul 2002, In: Protein Science. 11, 7, p. 1779-1787 9 p.

The major birch allergen, Bet v 1, shows affinity for a broad spectrum of physiological ligands

Mogensen, J. E., Wimmer, R., Larsen, J. N., Spangfort, M. D. & Otzen, D. E., 28 Jun 2002, In: Journal of Biological Chemistry. 277, 26, p. 23684-23692 9 p.

Burst-phase expansion of native protein prior to global unfolding in SDS

Otzen, D. E. & Oliveberg, M., 1 Jan 2002, In: Journal of Molecular Biology. 315, 5, p. 1231-1240 10 p.

Conformational plasticity in folding of the split β-α-β protein S6: Evidence for burst-phase disruption of the native state

Otzen, D. E. & Oliveberg, M., 1 Jan 2002, In: Journal of Molecular Biology. 317, 4, p. 613-627 15 p.

Folding of circular permutants with decreased contact order: General trend balanced by protein stability

Lindberg, M. O., Tångrot, J., Otzen, D. E., Dolgikh, D. A., Finkelstein, A. V. & Oliveberg, M., 7 Dec 2001, In: Journal of Molecular Biology. 314, 4, p. 891-900 10 p.

A simple way to measure protein refolding rates in water

Otzen, D. E. & Oliveberg, M., 26 Oct 2001, In: Journal of Molecular Biology. 313, 3, p. 479-483 5 p.

Stability and Folding of Endoglucanase I (CEL7B) from Humicoia Insolens

Otzen, D. & Schülein, M., 1 Jan 2001, In: Biocatalysis and Biotransformation. 19, 42130, p. 469-487 19 p.

Designed protein tetramer zipped together with a hydrophobic Alzheimer homology: A structural clue to amyloid assembly

Otzen, D. E., Kristensen, O. & Oliveberg, M., 29 Aug 2000, In: Proceedings of the National Academy of Sciences. 97, 18, p. 9907-9912 6 p.

Model process for separation based on unfolding and refolding of chymotrypsin inhibitor 2 in thermoseparating polymer two-phase systems

Umakoshi, H., Persson, J., Kroon, M., Johansson, H. O., Otzen, D. E., Kuboi, R. & Tjerneld, F., 23 Jun 2000, In: Journal of Chromatography B: Biomedical Sciences and Applications. 743, 1-2, p. 13-19 7 p.

The spectral and thermodynamic properties of staphylococcal enterotoxin A, E, and variants suggest that structural modifications are important to control their function

Cavallin, A., Arozenius, H., Kristensson, K., Antonsson, P., Otzen, D. E., Björk, P. & Forsberg, G., 21 Jan 2000, In: Journal of Biological Chemistry. 275, 3, p. 1665-1672 8 p.

Salt-induced detour through compact regions of the protein folding landscape

Otzen, D. E. & Oliveberg, M., 12 Oct 1999, In: Proceedings of the National Academy of Sciences. 96, 21, p. 11746-11751 6 p.

Electrostatics in the active site of an α-amylase

Nielsen, J. E., Beier, L., Otzen, D., Borchert, T. V., Frantzen, H. B., Andersen, K. V. & Svendsen, A., 15 Sept 1999, In: European Journal of Biochemistry. 264, 3, p. 816-824 9 p.

Structural changes in the transition state of protein folding: Alternative interpretations of curved chevron plots

Otzen, D. E., Kristensen, O., Proctor, M. & Oliveberg, M., 18 May 1999, In: Biochemistry. 38, 20, p. 6499-6511 13 p.

Analysis of protein-protein interactions by mutagenesis: Direct versus indirect effects

Otzen, D. E. & Fersht, A. R., 2 Feb 1999, In: Protein Engineering. 12, 1, p. 41-45 5 p.

A comparative study of the unfolding of the endoglucanase Ce145 from Humicola insolens in denaturant and surfactant

Otzen, D. E., Christiansen, L. & Schülein, M., 1 Jan 1999, In: Protein Science. 8, 9, p. 1878-1887 10 p.

Properties of PEG-modified microbial proteases. Activity and stability studies

Fatum, T. M., Olsen, A. A., Fuglsang, C. C. & Otzen, D., 1 Dec 1998, In: Progress in Biotechnology. 15, C, p. 147-150 4 p.

Folding of circular and permuted chymotrypsin inhibitor 2: Retention of the folding nucleus

Otzen, D. E. & Fersht, A. R., 2 Jun 1998, In: Biochemistry. 37, 22, p. 8139-8146 8 p.

Enzymatic characterisation of novamyl®, a thermostable α-amylase

Christophersen, C., Otzen, D. E., Norman, B. E., Christensen, S. & Schäfer, T., 1 Jan 1998, In: Starch/Staerke. 50, 1, p. 39-45 7 p.

Hydrogen exchange in chymotrypsin inhibitor 2 probed by mutagenesis

Neira, J. L., Itzhaki, L. S., Otzen, D. E., Davis, B. & Fersht, A. R., 4 Jul 1997, In: Journal of Molecular Biology. 270, 1, p. 99-110 12 p.

Rate of isomerisation of peptidyl-proline bonds as a probe for interactions in the physiological denatured state of chymotrypsin inhibitor 2

Tan, Y. J., Oliveberg, M., Otzen, D. E. & Fersht, A. R., 20 Jun 1997, In: Journal of Molecular Biology. 269, 4, p. 611-622 12 p.

Structure of the transition state for folding of a protein derived from experiment and simulation

Daggett, V., Li, A., Itzhaki, L. S., Otzen, D. E. & Fersht, A. R., 29 Mar 1996, In: Journal of Molecular Biology. 257, 2, p. 430-440 11 p.

Conformational pathway of the polypeptide chain of chymotrypsin inhibitor-2 growing from its N terminus in vitro. Parallels with the protein folding pathway

De Prat Gay, G., Ruiz-Sanz, J., Neira, J. L., Corrales, F. J., Otzen, D. E., Ladurner, A. G. & Fersht, A. R., 15 Dec 1995, In: Journal of Molecular Biology. 254, 5, p. 968-979 12 p.

The structure of the transition state for folding of chymotrypsin inhibitor 2 analysed by protein engineering methods: Evidence for a nucleation-condensation mechanism for protein folding

Itzhaki, L. S., Otzen, D. E. & Fersht, A. R., 24 Nov 1995, In: Journal of Molecular Biology. 254, 2, p. 260-288 29 p.

Side-Chain Determinants of β-Sheet Stability

Otzen, D. E. & Fersht, A. R., 1 May 1995, In: Biochemistry. 34, 17, p. 5718-5724 7 p.

Movement of the Position of the Transition State in Protein Folding

Matouschek, A., Otzen, D. E., Itzhaki, L. S., Jackson, S. E. & Fersht, A. R., 1 Jan 1995, In: Biochemistry. 34, 41, p. 13656-13662 7 p.

Nature and Consequences of GroEL-Protein Interactions

Itzhaki, L. S., Otzen, D. E. & Fersht, A. R., 1 Jan 1995, In: Biochemistry. 34, 44, p. 14581-14587 7 p.

Protein Fragments as Models for Events in Protein Folding Pathways: Protein Engineering Analysis of the Association of Two Complementary Fragments of the Barley Chymotrypsin Inhibitor 2 (CI-2)

Ruiz-Sanz, J., de Prat Gay, G., Otzen, D. E. & Fersht, A. R., 1 Jan 1995, In: Biochemistry. 34, 5, p. 1695-1701 7 p.

Structural Factors Contributing to the Hydrophobic Effect: The Partly Exposed Hydrophobic Minicore in Chymotrypsin Inhibitor

Otzen, D. E., Rheinnecker, M. & Fersht, A. R., 1 Jan 1995, In: Biochemistry. 34, 40, p. 13051-13058 8 p.

Single versus parallel pathways of protein folding and fractional formation of structure in the transition state

Fersht, A. R., Itzhaki, L. S., Elmasry, N. F., Matthews, J. M. & Otzen, D. E., 25 Oct 1994, In: Proceedings of the National Academy of Sciences. 91, 22, p. 10426-10429 4 p.

Structure of the transition state for the folding/unfolding of the barley chymotrypsin inhibitor 2 and its implications for mechanisms of protein folding

Otzen, D. E., Itzhaki, L. S., Elmasry, N. F., Jackson, S. E. & Fersht, A. R., 25 Oct 1994, In: Proceedings of the National Academy of Sciences. 91, 22, p. 10422-10425 4 p.

Dual hydrolytic role for Pb(II) ions

Otzen, D. E., Barciszewski, J. & Clark, B. F. C., 1 Jan 1994, In: Biochimie. 76, 1, p. 15-21 7 p.

Altered lead(II)-cleavage pattern of free Phe-tRNA(Phe) and Phe-tRNA(Phe) in ternary complex with EF-Tu:GTP

Otzen, D. E., Barciszewski, J. & Clark, B. F. C., 1 Jan 1993, In: Biochemistry and Molecular Biology International. 31, 1, p. 95-103 9 p.

Reduction in the amount of 8-hydroxy-2´-deoxyguanosine in the DNA of SV40-transformed human fibroblasts as compared with normal cells in culture.

Barciszewski, J., Rattan, S., Siboska, G., Otzen, D. & Clark, B. F. C., 1993, In: F E B S Letters. 318, p. 186-188

Specific incorporation of kinetin into eukaryotic and prokaryotic transfer ribonucleic acid molecules

Barciszewski, J., Otzen, D., Rattan, S. I. S. & Clark, B. F. C., 1 Dec 1992, In: Biochemistry international. 28, 5, p. 805-811 7 p.

Specific incorportation of kinetin into eukaryotic and prokaryotic transfer ribonucleic acid molecules.

Barciszewski, J., Rattan, S., Otzen, D. & Clark, B. F. C., 1992, In: Biochemistry international. 28, p. 805-811