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Claus Oxvig

Simple and reliable procedure for PCR amplification of genomic DNA from yeast cells using short sequencing primers.

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  • Department of Molecular Biology
Yeast is widely used in molecular biology. Heterologous expression of recombinant proteins in yeast involves screening of a large number of recombinants. We present an easy and reliable procedure for amplifying genomic DNA from freshly grown cells of the methylotrophic yeast Pichia pastoris by means of PCR without any prior DNA purification steps. This method involves a simple boiling step of whole yeast cells in the presence of detergent, and subsequent amplification of genomic DNA using short sequencing primers in a polymerase chain reaction assay with a decreasing annealing temperature.
Udgivelsesdato: 1997-Jun
Original languageEnglish
JournalIUBMB Life
Volume42
Issue1
Pages (from-to)169-72
Number of pages3
ISSN1521-6543
Publication statusPublished - 1997

    Research areas

  • Base Sequence, DNA Primers, DNA, Fungal, Detergents, Heat, Pichia, Polymerase Chain Reaction, Templates, Genetic

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