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Claus Oxvig

Complete cDNA sequence of the preproform of human pregnancy-associated plasma protein-A. Evidence for expression in the brain and induction by cAMP.

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  • Jesper Haaning, Department of Molecular and Structural Biology, University of Aarhus, Denmark
  • Claus Oxvig
  • Michael Toft Overgaard, Denmark
  • Peter Ebbesen, Department of Virus and Cancer, Danish Cancer Society, Denmark
  • Torsten Kristensen, Denmark
  • Lars Sottrup-Jensen
A cDNA that encodes the prepropeptide of pregnancy-associated plasma protein-A (preproPAPP-A), a putative metalloproteinase, has been cloned and sequenced. PAPP-A is synthesized in the placenta as a 1627-residue precursor preproprotein with a putative 22-residue signal peptide and a highly basic propeptide of 58 residues. The preproPAPP-A–encoding transcript contains a region with an extremely high G+C content and has an unusually long 5′ untranslated region with several upstream short ORF. No alternatively spliced products could be identified by means of Northern blotting experiments or with rapid amplification of 5′ cDNA ends experiments. A stretch within the 5′ untranslated region shows sequence identities to a partial cDNA isolated from brain and a to cAMP-inducible sequence from a choriocarcinoma cell line.
Original languageEnglish
JournalEuropean Journal of Biochemistry
Volume237
Issue1
Pages (from-to)159-163
Number of pages4
ISSN0014-2956
DOIs
Publication statusPublished - Apr 1996

    Research areas

  • 8-Bromo Cyclic Adenosine Monophosphate, Amino Acid Sequence, Base Sequence, Brain, Cell Line, Cloning, Molecular, DNA, Complementary, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Pregnancy-Associated Plasma Protein-A, Protein Precursors, Trophoblasts

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