Claus Munck Petersen

Surface membrane CD4 turnover in phorbol ester stimulated T-lymphocytes. Evidence of degradation and increased synthesis

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Down-regulation of surface membrane CD4 (smCD4) in phorbol ester stimulated T-cells resulted from internalization. Internalization (T1/2 = 15 min at 50 ng PMA/ml) was followed by degradation of CD4-bound antibodies. Degradation in unstimulated T-cells was comparatively insignificant. Release of degradation products was PMA dose-dependent and could be inhibited by methylamine. Uptake and degradation continued after maximal down-regulation of surface membrane CD4, and methylamine did not inhibit reappearance of smCD4 antigens. Metabolic labelling of T-cells further showed that ongoing synthesis rather than recycling contributed to an accelerated smCD4 turnover in activated cells.
Original languageEnglish
JournalF E B S Letters
Volume276
Issue1-2
Pages (from-to)59-62
Number of pages4
ISSN0014-5793
Publication statusPublished - 1990

    Research areas

  • Antibodies, Monoclonal, Antigens, CD4, Cells, Cultured, Humans, Kinetics, Lymphocyte Activation, T-Lymphocytes, Tetradecanoylphorbol Acetate

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