Claus Munck Petersen

Soluble tumor necrosis factor (TNF) receptors conserve TNF bioactivity in meningitis patient spinal fluid

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The content of tumor necrosis factor (TNF)-alpha antigen and the bioactivity of soluble TNF receptor type II (sTNF-RII) in cerebrospinal fluid (CSF) from 29 patients with meningeal symptoms and fever were examined. Immunoreactive TNF was demonstrated in CSF from 4 of 7 patients with bacterial meningitis. In 3 of 8 patients with aseptic meningitis, CSF also contained TNF, but TNF bioactivity was confined to samples from patients with bacterial meningitis. Bioactive TNF was exclusively in high-performance liquid chromatography fractions containing 30- to 60-kDa proteins. Lipopolysaccharide induced down-regulation, possibly after shedding of granulocyte surface membrane TNF-RII. Consistently, there was a statistically significant correlation between sTNF-RII and CSF leukocyte counts. Bioactive TNF was found only in CSF containing >1 ng of sTNF-RII/mL; samples without TNF bioactivity contained less sTNF-RII. Thus, a stabilizing effect of sTNF-RII on the oligomeric cytokine in vivo is plausible.
Original languageEnglish
JournalJournal of Infectious Diseases
Volume174
Issue3
Pages (from-to)557-63
Number of pages7
ISSN0022-1899
Publication statusPublished - 1996

    Research areas

  • Cells, Cultured, Cerebrospinal Fluid, Chromatography, High Pressure Liquid, Down-Regulation, Granulocytes, Humans, Leukocyte Count, Lipopolysaccharides, Meningitis, Aseptic, Meningitis, Bacterial, Receptors, Tumor Necrosis Factor, Tumor Necrosis Factor-alpha

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