Claus Munck Petersen

Immunocytochemical identification of the human alpha 2-macroglobulin receptor in monocytes and fibroblasts: monoclonal antibodies define the receptor as a monocyte differentiation antigen

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  • Department of Anatomy
  • Department of Human Genetics
  • Department of Medical Biochemistry
The alpha 2-macroglobulin receptor was recently purified from rat liver and human placenta. Three different monoclonal antibodies have now been raised against the human receptor and expression of the 440-kDa receptor protein is demonstrated in human placenta, fibroblasts, liver, and monocytes by immunoblot analysis. Flow cytometric studies showed that anti-alpha 2-macroglobulin receptor monoclonal antibodies bind to 90-100% of the blood monocyte population and not to other blood cells. This defines the alpha 2-macroglobulin receptor as a monocyte differentiation antigen, different from any of the classified leucocyte cluster determinants. Electron microscopic gold immunocytochemistry revealed the subcellular distribution of the receptor in human cultured monocytes and fibroblasts. In these cells, 18-33% of the gold particles were found on the outside of the plasma membrane, and in fibroblasts, especially, in coated invaginations. The intracellular receptors were mainly distributed in vesicles and tubular structures.
Original languageEnglish
JournalExperimental Cell Research
Volume190
Issue2
Pages (from-to)195-203
Number of pages8
ISSN0014-4827
Publication statusPublished - 1990

    Research areas

  • Antibodies, Monoclonal, Antigens, Differentiation, Biological Markers, Cells, Cultured, Fibroblasts, Flow Cytometry, Fluorescent Antibody Technique, Humans, Immunohistochemistry, LDL-Receptor Related Protein 1, Macrophages, Microscopy, Electron, Monocytes, Receptors, Immunologic

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ID: 19903814