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Claus Krogh Madsen

Assistant Professor

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Claus Krogh Madsen

Assistant Professor

  • Department of Agroecology - Crop Genetics and Biotechnology
Postal address:
Forsøgsvej 1

Email: clauskrogh.madsen@agro.au.dk


2012-presentPost doc at Dept. of Molecular Biology and Genetics, AU on the Bio4Bio project.
2012Post doc at Dept. of Molecular Biology and Genetics, AU, on a proof of concept project.
2008-2011Ph.D. student at Dept. of Molecular Biology and Genetics, AU.


2011Ph.D. in plant molecular biology, Aarhus University
2007M.Sc. in biotechnology, Aalborg University

Scientific summary

My work concerns the improvement of cereals and grasses with molecular methods. This can be either directly i.e. plant transformation or indirectly by identifying molecular targets for conventional breeding. My Ph.D work focused on the endogenous cereal phytases. Some cereals e.g. rye and wheat which belong to the Triticeae tribe have high phytase activity in the mature grains. This can be beneficial for the uptake of minerals when the grains are used in food or feed. I cloned the responsible PAPhy genes from crop, relict and wild Triticeae and studied the evolutionary history of the PAPhy’s in order to understand how the high phytase trait came about and find ways to improve it further.
My current work concerns the involvement of laccases in cell wall lignification. Plant laccases are multigene families with e.g. 17 and 14 members in Arabidopsis thaliana and maize (Zea mays L.) respectively. The functional assignment of individual laccases in relation to cell wall lignification is therefore a major challenge, but also an opportunity. Thus, laccases may offer a route to modulate cell wall recalcitrance while maintaining agronomical performance. This would be highly valuable in energy- as well as forage crops. My studies are therefore performed on perennial ryegrass (Lolium perenne L.) which is an important forage- and potential energy crop. Key methods in my work are molecular cloning, plant transformation, gene discovery by e.g. library screening and PCR based methods, qPCR, and recombinant expression of plant proteins in Pichia pastoris.

Bibliometric summary (Complete list available in PURE)

Madsen, C. K., & Brinch-Pedersen, H. (2020). Globoids and Phytase: The Mineral Storage and Release System in Seeds. International Journal of Molecular Sciences , 21(20), [7519]. https://doi.org/10.3390/ijms21207519
Holme, I., Madsen, C. K., Wendt, T., & Brinch-Pedersen, H. (2020). Horizontal Stacking of PAPhy_a Cisgenes in Barley Is a Potent Strategy for Increasing Mature Grain Phytase Activity. Frontiers in Plant Science, 11, [592139]. https://doi.org/10.3389/fpls.2020.592139
Madsen, C. K., & Brinch-Pedersen, H. (2020). A novel wheat q’ allele identified by forward genetic in silico TILLING. Journal of Plant Physiology, 251, [153221]. https://doi.org/10.1016/j.jplph.2020.153221
Bekalu, Z. E., Madsen, C. K., Dionisio, G., Holme, I. B., Jørgensen, L. N., Fomsgaard, I. S., & Brinch-Pedersen, H. (2020). Overexpression of nepenthesin HvNEP-1 in barley endosperm reduces fusarium head blight and mycotoxin accumulation. Agronomy, 10(2), [203]. https://doi.org/10.3390/agronomy10020203
Madsen, C. K., Brearley, C. A., & Brinch-Pedersen, H. (2019). Lab-scale preparation and QC of phytase assay substrate from rice bran. Analytical Biochemistry, 578, 7-12. https://doi.org/10.1016/j.ab.2019.04.021
Nørgaard, J. V., Malla, N., Dionisio, G., Madsen, C. K., Pettersson, D., Lærke, H. N., Hjortshøj, R. L., & Brinch-Pedersen, H. (2019). Exogenous xylanase or protease for pigs fed barley cultivars with high or low enzyme inhibitors. Animal Feed Science and Technology, 248, 59-66. https://doi.org/10.1016/j.anifeedsci.2018.12.005
Whankaew, S., Ruttajorn, K., Madsen, C. K., Asp, T., Xu, L., Nakkaew, A., & Phongdara, A. (2019). An EgHd3a-like and its alternatively spliced transcripts in the oil palm (Elaeis guineensis). Songklanakarin Journal of Science and Technology (SJST), 41(2), 332-340. https://doi.org/10.14456/sjst-psu.2019.42
Krogh Madsen, C., Pettersson, D., Hjortshøj, R., Katholm, A., & Brinch-Pedersen, H. (2018). Superior Growth Rates in Broilers Fed Wheat with Low In Vitro Feed-Xylanase Inhibition. Journal of Agricultural and Food Chemistry, 66(16), 4044-4050. https://doi.org/10.1021/acs.jafc.8b00208
Madsen, C. K., Petersen, G., Seberg, O., & Brinch-Pedersen, H. (2017). Evolution and diversity of PAPhy_a phytase in the genepool of wheat (Triticum aestivum L., Poaceae). Genetic Resources and Crop Evolution, 64(8), 2115-2126. https://doi.org/10.1007/s10722-017-0501-9
Zor, K., Mark, C., Heiskanen, A., Madsen, C. K., Dufva, M., Emnéus, J., Brinch-Pedersen, H., & Finnie, C. (2017). Immobilisation of barley aleurone layers enables parallelisation of assays and analysis of transient gene expression in single cells. Plant Physiology and Biochemistry, 118, 71-76. https://doi.org/10.1016/j.plaphy.2017.06.008
Scholey, D., Burton, E., Morgan, N., Sanni, C., Madsen, C. K., Dionisio, G., & Brinch-Pedersen, H. (2017). P and Ca digestibility is increased in broiler diets supplemented with the high-phytase HIGHPHY wheat. Animal, 11(9), 1457-1463. https://doi.org/10.1017/S1751731117000544
Bekalu, Z. E., Madsen, C. K., Dionisio, G., & Brinch-Pedersen, H. (2017). Aspergillus ficuum phytase activity is inhibited by cereal grain components. PLOS ONE, 12(5), [e0176838]. https://doi.org/10.1371/journal.pone.0176838
Holme, I., Dionisio, G., Madsen, C. K., & Brinch-Pedersen, H. (2017). Barley HvPAPhy_a as transgene provides high and stable phytase activities in mature barley straw and in grains. Plant Biotechnology Journal, 15(4), 415-422. https://doi.org/10.1111/pbi.12636
Madsen, C. K., Vismans, G., & Brinch-Pedersen, H. (2016). The PARS sequence increase the efficiency of stable Pichia pastoris transformation. Journal of Microbiological Methods, 129, 1-7. https://doi.org/10.1016/j.mimet.2016.07.015
Kronbak, R., Ingvardsen, C. R., Madsen, C. K., & Gregersen, P. L. (2014). A novel approach to the generation of seamless constructs for plant transformation. Plant Methods, 10(1), 1-10. [10]. https://doi.org/10.1186/1746-4811-10-10
Brinch-Pedersen, H., Madsen, C. K., Holme, I. B., & Dionisio, G. (2014). Increased understanding of the cereal phytase complement for better mineral bio-availability and resource management. Journal of Cereal Science, 59(3), 373-381. https://doi.org/10.1016/j.jcs.2013.10.003
Madsen, C. K., Dionisio, G., Holme, I., Holm, P. B., & Brinch-Pedersen, H. (2013). High mature grain phytase activity in the Triticeae has evolved by duplication followed by neofunctionalization of the purple acid phosphatase phytase (PAPhy) gene. Journal of Experimental Botany, 64(11), 3111-3123. https://doi.org/10.1093/jxb/ert116
Holme, I., Dionisio, G., Brinch-Pedersen, H., Wendt, T., Madsen, C. K., Vincze, É., & Holm, P. B. (2012). A Cisgenic Approach for Improving the Bioavailability of Phosphate in the Barley Grain. ISB NEWS REPORT, March, 8-11. http://www.nbiap.vt.edu/isb-news-report.aspx
Holme, I., Dionisio, G., Brinch-Pedersen, H., Wendt, T., Madsen, C. K., Vincze, É., & Holm, P. B. (2012). Cisgenic barley with improved phytase activity. Plant Biotechnology Journal, 10(2), 237-247. https://doi.org/10.1111/j.1467-7652.2011.00660.x
Madsen, C. K. (2011). Unraveling the major triticeae phytases – towards cereals with high grain phytase activity. Aarhus University, Science and Technology.
Dionisio, G., Madsen, C. K., Holm, P. B., Welinder, K. G., Jørgensen, M., Stoger, E., Arcalis, E., & Brinch-Pedersen, H. (2011). Cloning and Characterization of Purple Acid Phosphatase Phytases from Wheat, Barley, Maize and Rice. Plant Physiology, 156(3), 1087-1100. https://doi.org/10.1104/pp.110.164756
Hansen, F. T., Madsen, C. K., Nordland, A. M., Grasser, M., Merkle, T., & Grasser, K. D. (2008). A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry, 47(50), 13207-13214. https://doi.org/10.1021/bi801772k


I have participated as a speaker in the 6th European Symposium on Enzymes in Grain Processing (2011).


My Ph.D work has resulted in patent applications PCT/EP2012/057515 and US 61/479,689 High expression cereal phytase gene.

Knowledge exchange

I have given lectures at local high schools and hosted talented high school students who did part of their exam projects in our lab. Further, I have contributed to a high school textbook; Bioteknologi –En temabog. SYSTIME 2011, ISBN 9788761630957.