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Clarissa Schwab

Colonization of Cutibacterium Avidum During Infant Gut Microbiota Establishment

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  • Vanesa Natalin Rocha Martin, ETH Zürich, University of Zurich
  • ,
  • Clarissa Schwab
  • Lukasz Krych, University of Copenhagen
  • ,
  • Evelyn Voney, ETH Zürich
  • ,
  • Annelies Geirnaert, ETH Zürich
  • ,
  • Christian Braegger, University of Zurich
  • ,
  • Christophe Lacroix, ETH Zürich

Establishment of the infant gut microbiota affects gut maturation and influences long-term health. Cutibacterium (formerly Propionibacterium) have been identified as early colonizers, but little is known about their function. Using a cultivation-dependent and -independent approach, we determined Cutibacterium prevalence, diversity and functional potential. In feces from a Swiss infant cohort (n = 38), prevalence of Propionibacterium/Cutibacterium decreased from 84% at 2 weeks, to 65% at 4 weeks, 47% at 8 weeks and 41% at 12 weeks of age. Abundance varied among individuals, and persistence depended on the colonization levels at 2 weeks. Cutibacterium isolates (n = 87) were obtained from 10 infants from a smaller cohort (n = 12); restriction fragment length polymorphism clustered isolates in four groups, and all identified as Cutibacterium avidum. Colonization potential and metabolic effects of C. avidum addition were tested in an in vitro continuous intestinal fermentation model mimicking infant proximal colon conditions. Cutibacterium avidum spiked daily at 10 8 or 10 9 cells mL -1 colonized, decreased formate and persisted during the washout period. Significant correlations were observed between Propionibacterium/Cutibacterium and lactate-producers and protein-degraders in both reactors and infant feces. Our findings highlight the natural presence of C. avidum and its role as a lactate-consumer and propionate-producer in infants younger than 3 months.

Original languageEnglish
Article numberfiy215
JournalFEMS Microbiology Ecology
Volume95
Issue1
Number of pages14
ISSN0168-6496
DOIs
Publication statusPublished - 1 Jan 2019
Externally publishedYes

    Research areas

  • Cutibacterium, In vitro, Infant gut microbiota, Lactate, Propionate, Propionibacterium

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ID: 191591582