Christiane Beer

Multi-platform Genotoxicity Analysis of Silver Nanoparticles in The Model Cell Line CHO-K1

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Multi-platform Genotoxicity Analysis of Silver Nanoparticles in The Model Cell Line CHO-K1. / Jiang, Xiumei; Foldbjerg, Rasmus; Miclaus, Teodora; Wang, Liming; Singh, Rajinder; Hayashi, Yuya; Sutherland, Duncan; Chen, Chunying; Autrup, Herman; Beer, Christiane.

In: Toxicology Letters, 17.07.2013.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Jiang, Xiumei ; Foldbjerg, Rasmus ; Miclaus, Teodora ; Wang, Liming ; Singh, Rajinder ; Hayashi, Yuya ; Sutherland, Duncan ; Chen, Chunying ; Autrup, Herman ; Beer, Christiane. / Multi-platform Genotoxicity Analysis of Silver Nanoparticles in The Model Cell Line CHO-K1. In: Toxicology Letters. 2013.

Bibtex

@article{8c1cd7c85a2c4effad5fc0868d6eda0c,
title = "Multi-platform Genotoxicity Analysis of Silver Nanoparticles in The Model Cell Line CHO-K1",
abstract = "Investigation of the genotoxic potential of nanomaterials is essential to evaluate if they pose a cancer risk for exposed workers and consumers. The Chinese hamster ovary cell line CHO-K1 is recommended by the OECD for use in the micronucleus assay and is commonly used for genotoxicity testing. However, studies investigating if this cell line is suitable for the genotoxic evaluation of nanomaterials, including induction of DNA adduct and micronuclei formation, are rare and for silver nanoparticles (Ag NPs) missing. Therefore, we here systematically investigated DNA and chromosomal damage induced by BSA coated Ag NPs (15.9±7.6nm) in CHO-K1 cells in relation to cellular uptake and intracellular localization, their effects on mitochondrial activity and production of reactive oxygen species (ROS), cell cycle, apoptosis and necrosis. Ag NPs are taken up by CHO-K1 cells and are presumably translocated into endosomes/lysosomes. Our cytotoxicity studies demonstrated a concentration-dependent decrease of mitochondrial activity and increase of intracellular reactive oxygen species (ROS) in CHO-K1 cells following exposure to Ag NPs and Ag(+) (0-20μg/ml) for 24h. Annexin V/propidium iodide assay showed that Ag NPs and Ag(+) induced apoptosis and necrosis, which is in agreement with an increased fraction of cells in subG1 phase of the cell cycle. Genotoxicity studies showed, that Ag NPs but also silver ions (Ag(+)) induced bulky-DNA adducts, 8-oxodG and micronuclei formation in a concentration-dependent manner, however, there were quantitative and qualitative differences between the particulate and ionic form of silver. Taken together, our multi-platform genotoxicity and cytotoxicity analysis demonstrates that CHO-K1 cells are suitable for the investigation of genotoxicity of nanoparticles like Ag NPs.",
author = "Xiumei Jiang and Rasmus Foldbjerg and Teodora Miclaus and Liming Wang and Rajinder Singh and Yuya Hayashi and Duncan Sutherland and Chunying Chen and Herman Autrup and Christiane Beer",
note = "Copyright {\textcopyright} 2013. Published by Elsevier Ireland Ltd.",
year = "2013",
month = jul,
day = "17",
doi = "10.1016/j.toxlet.2013.07.011",
language = "English",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier Ireland Ltd.",

}

RIS

TY - JOUR

T1 - Multi-platform Genotoxicity Analysis of Silver Nanoparticles in The Model Cell Line CHO-K1

AU - Jiang, Xiumei

AU - Foldbjerg, Rasmus

AU - Miclaus, Teodora

AU - Wang, Liming

AU - Singh, Rajinder

AU - Hayashi, Yuya

AU - Sutherland, Duncan

AU - Chen, Chunying

AU - Autrup, Herman

AU - Beer, Christiane

N1 - Copyright © 2013. Published by Elsevier Ireland Ltd.

PY - 2013/7/17

Y1 - 2013/7/17

N2 - Investigation of the genotoxic potential of nanomaterials is essential to evaluate if they pose a cancer risk for exposed workers and consumers. The Chinese hamster ovary cell line CHO-K1 is recommended by the OECD for use in the micronucleus assay and is commonly used for genotoxicity testing. However, studies investigating if this cell line is suitable for the genotoxic evaluation of nanomaterials, including induction of DNA adduct and micronuclei formation, are rare and for silver nanoparticles (Ag NPs) missing. Therefore, we here systematically investigated DNA and chromosomal damage induced by BSA coated Ag NPs (15.9±7.6nm) in CHO-K1 cells in relation to cellular uptake and intracellular localization, their effects on mitochondrial activity and production of reactive oxygen species (ROS), cell cycle, apoptosis and necrosis. Ag NPs are taken up by CHO-K1 cells and are presumably translocated into endosomes/lysosomes. Our cytotoxicity studies demonstrated a concentration-dependent decrease of mitochondrial activity and increase of intracellular reactive oxygen species (ROS) in CHO-K1 cells following exposure to Ag NPs and Ag(+) (0-20μg/ml) for 24h. Annexin V/propidium iodide assay showed that Ag NPs and Ag(+) induced apoptosis and necrosis, which is in agreement with an increased fraction of cells in subG1 phase of the cell cycle. Genotoxicity studies showed, that Ag NPs but also silver ions (Ag(+)) induced bulky-DNA adducts, 8-oxodG and micronuclei formation in a concentration-dependent manner, however, there were quantitative and qualitative differences between the particulate and ionic form of silver. Taken together, our multi-platform genotoxicity and cytotoxicity analysis demonstrates that CHO-K1 cells are suitable for the investigation of genotoxicity of nanoparticles like Ag NPs.

AB - Investigation of the genotoxic potential of nanomaterials is essential to evaluate if they pose a cancer risk for exposed workers and consumers. The Chinese hamster ovary cell line CHO-K1 is recommended by the OECD for use in the micronucleus assay and is commonly used for genotoxicity testing. However, studies investigating if this cell line is suitable for the genotoxic evaluation of nanomaterials, including induction of DNA adduct and micronuclei formation, are rare and for silver nanoparticles (Ag NPs) missing. Therefore, we here systematically investigated DNA and chromosomal damage induced by BSA coated Ag NPs (15.9±7.6nm) in CHO-K1 cells in relation to cellular uptake and intracellular localization, their effects on mitochondrial activity and production of reactive oxygen species (ROS), cell cycle, apoptosis and necrosis. Ag NPs are taken up by CHO-K1 cells and are presumably translocated into endosomes/lysosomes. Our cytotoxicity studies demonstrated a concentration-dependent decrease of mitochondrial activity and increase of intracellular reactive oxygen species (ROS) in CHO-K1 cells following exposure to Ag NPs and Ag(+) (0-20μg/ml) for 24h. Annexin V/propidium iodide assay showed that Ag NPs and Ag(+) induced apoptosis and necrosis, which is in agreement with an increased fraction of cells in subG1 phase of the cell cycle. Genotoxicity studies showed, that Ag NPs but also silver ions (Ag(+)) induced bulky-DNA adducts, 8-oxodG and micronuclei formation in a concentration-dependent manner, however, there were quantitative and qualitative differences between the particulate and ionic form of silver. Taken together, our multi-platform genotoxicity and cytotoxicity analysis demonstrates that CHO-K1 cells are suitable for the investigation of genotoxicity of nanoparticles like Ag NPs.

U2 - 10.1016/j.toxlet.2013.07.011

DO - 10.1016/j.toxlet.2013.07.011

M3 - Journal article

C2 - 23872614

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

ER -