Aarhus University Seal / Aarhus Universitets segl

Carsten Scavenius

Human cornea proteome: Identification and quantitation of the proteins of the three main layers including epithelium, stroma and endothelium

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Diseases of the cornea are common and refer to conditions like infections, injuries and genetic defects. Morphologically, many corneal diseases affect only certain layers of the cornea and separate analysis of the individual layers is therefore of interest to explore the basic molecular mechanisms involved in corneal health and disease. In this study, the three main layers including, the epithelium, stroma and endothelium of healthy human corneas were isolated. Prior to analysis by LC-MS/MS the proteins from the different layers were either (i) separated by SDS-PAGE followed by in-gel trypsinization, (ii) in-solution digested without prior protein separation or, (iii) in-solution digested followed by cation exchange chromatography. A total of 3250 unique Swiss-Prot annotated proteins were identified in human corneas, 2737 in the epithelium, 1679 in the stroma and 880 in the endothelial layer. Of these, 1787 proteins have not previously been identified in the human cornea by mass spectrometry. In total, 771 proteins were quantified, 157 based on in-solution digestion and 770 based on SDS-PAGE separation followed by in-gel digestion of excised gel pieces. Protein analysis showed that many of the identified proteins are plasma proteins involved in defense responses.
Original languageEnglish
JournalJournal of Proteome Research
Pages (from-to)4231–4239
Number of pages9
Publication statusPublished - 10 Jul 2012

    Research areas

  • human cornea, epithelium; stroma, endothelium; proteomics, label free quantitation, TripleTOF 5600

See relations at Aarhus University Citationformats



    Activity: Participating in or organising an event typesParticipation in or organisation af a conference

ID: 45823853