Bo Martin Bibby

Metallic silver fragments cause massive tissue loss in the mouse brain

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Metallic silver fragments cause massive tissue loss in the mouse brain. / Locht, Linda Jansons; Pedersen, Mie; Markholt, Sara; Bibby, Bo Martin; Larsen, Agnete; Penkowa, Milena; Stoltenberg, Meredin; Rungby, Jørgen.

In: Basic & Clinical Pharmacology & Toxicology, Vol. 109, No. 1, 07.2011, p. 1-10.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Locht, LJ, Pedersen, M, Markholt, S, Bibby, BM, Larsen, A, Penkowa, M, Stoltenberg, M & Rungby, J 2011, 'Metallic silver fragments cause massive tissue loss in the mouse brain', Basic & Clinical Pharmacology & Toxicology, vol. 109, no. 1, pp. 1-10. https://doi.org/10.1111/j.1742-7843.2010.00668.x

APA

Locht, L. J., Pedersen, M., Markholt, S., Bibby, B. M., Larsen, A., Penkowa, M., ... Rungby, J. (2011). Metallic silver fragments cause massive tissue loss in the mouse brain. Basic & Clinical Pharmacology & Toxicology, 109(1), 1-10. https://doi.org/10.1111/j.1742-7843.2010.00668.x

CBE

Locht LJ, Pedersen M, Markholt S, Bibby BM, Larsen A, Penkowa M, Stoltenberg M, Rungby J. 2011. Metallic silver fragments cause massive tissue loss in the mouse brain. Basic & Clinical Pharmacology & Toxicology. 109(1):1-10. https://doi.org/10.1111/j.1742-7843.2010.00668.x

MLA

Locht, Linda Jansons et al. "Metallic silver fragments cause massive tissue loss in the mouse brain". Basic & Clinical Pharmacology & Toxicology. 2011, 109(1). 1-10. https://doi.org/10.1111/j.1742-7843.2010.00668.x

Vancouver

Locht LJ, Pedersen M, Markholt S, Bibby BM, Larsen A, Penkowa M et al. Metallic silver fragments cause massive tissue loss in the mouse brain. Basic & Clinical Pharmacology & Toxicology. 2011 Jul;109(1):1-10. https://doi.org/10.1111/j.1742-7843.2010.00668.x

Author

Locht, Linda Jansons ; Pedersen, Mie ; Markholt, Sara ; Bibby, Bo Martin ; Larsen, Agnete ; Penkowa, Milena ; Stoltenberg, Meredin ; Rungby, Jørgen. / Metallic silver fragments cause massive tissue loss in the mouse brain. In: Basic & Clinical Pharmacology & Toxicology. 2011 ; Vol. 109, No. 1. pp. 1-10.

Bibtex

@article{35c10f4f32a84e8e86a05eeec5bbd698,
title = "Metallic silver fragments cause massive tissue loss in the mouse brain",
abstract = "Silver is a metal with well-known antibacterial effects. This makes silver an attractive coating material for medical devices for use inside the body, e.g. orthopaedic prostheses and catheters used in neurosurgery as it has been found to reduce the high risk of infections. Lately, the use of nano-silver particles in the industry, e.g. woven into fabrics and furniture has increased, and thus the exposure to silver particles in daily life increases. To study the effect of metallic silver particles on nervous tissue, we injected micron-sized silver particles into the mouse brain by stereotactic procedures. After 7, 14 days and 9 months, the silver-exposed animals had considerable brain damage seen as cavity formation and inflammation adjacent to the injected metallic silver particles. The tissue loss involved both cortical and hippocampal structures and resulted in enlargement of the lateral ventricles. Autometallographic silver enhancement showed silver uptake in lysosomes of glia cells and neurons in the ipsilateral cortex and hippocampus alongside a minor uptake on the contralateral side. Silver was also detected in ependymal cells and the choroid plexus. After 9 months, spreading of silver to the kidneys was seen. Cell counts of immunostained sections showed that metallic silver induced a statistically significant inflammatory response, i.e. increased microgliosis (7 days: p <0.0001; 14 days: p <0.01; 9 months: p <0.0001) and TNF-α expression (7 and 14 days: p <0.0001; 9 months: p = 0.91). Significant astrogliosis (7, 14 days and 9 months: p <0.0001) and increased metallothionein (MT I + II) expression (7 and 14 days: p <0.0001; 9 months: p <0.001) were also seen in silver-exposed brain tissue. We conclude that metallic silver implants release silver ions causing neuroinflammation and a progressive tissue loss in the brain.",
keywords = "Animals, Brain, Cell Count, Female, Gene Expression Regulation, Inflammation, Metallothionein, Mice, Mice, Inbred BALB C, Prostheses and Implants, Silver, Stereotaxic Techniques, Time Factors, Tissue Distribution",
author = "Locht, {Linda Jansons} and Mie Pedersen and Sara Markholt and Bibby, {Bo Martin} and Agnete Larsen and Milena Penkowa and Meredin Stoltenberg and J{\o}rgen Rungby",
note = "{\circledC} 2011 The Authors. Basic & Clinical Pharmacology & Toxicology {\circledC} 2011 Nordic Pharmacological Society.",
year = "2011",
month = "7",
doi = "10.1111/j.1742-7843.2010.00668.x",
language = "English",
volume = "109",
pages = "1--10",
journal = "Basic & Clinical Pharmacology & Toxicology",
issn = "1742-7835",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Metallic silver fragments cause massive tissue loss in the mouse brain

AU - Locht, Linda Jansons

AU - Pedersen, Mie

AU - Markholt, Sara

AU - Bibby, Bo Martin

AU - Larsen, Agnete

AU - Penkowa, Milena

AU - Stoltenberg, Meredin

AU - Rungby, Jørgen

N1 - © 2011 The Authors. Basic & Clinical Pharmacology & Toxicology © 2011 Nordic Pharmacological Society.

PY - 2011/7

Y1 - 2011/7

N2 - Silver is a metal with well-known antibacterial effects. This makes silver an attractive coating material for medical devices for use inside the body, e.g. orthopaedic prostheses and catheters used in neurosurgery as it has been found to reduce the high risk of infections. Lately, the use of nano-silver particles in the industry, e.g. woven into fabrics and furniture has increased, and thus the exposure to silver particles in daily life increases. To study the effect of metallic silver particles on nervous tissue, we injected micron-sized silver particles into the mouse brain by stereotactic procedures. After 7, 14 days and 9 months, the silver-exposed animals had considerable brain damage seen as cavity formation and inflammation adjacent to the injected metallic silver particles. The tissue loss involved both cortical and hippocampal structures and resulted in enlargement of the lateral ventricles. Autometallographic silver enhancement showed silver uptake in lysosomes of glia cells and neurons in the ipsilateral cortex and hippocampus alongside a minor uptake on the contralateral side. Silver was also detected in ependymal cells and the choroid plexus. After 9 months, spreading of silver to the kidneys was seen. Cell counts of immunostained sections showed that metallic silver induced a statistically significant inflammatory response, i.e. increased microgliosis (7 days: p <0.0001; 14 days: p <0.01; 9 months: p <0.0001) and TNF-α expression (7 and 14 days: p <0.0001; 9 months: p = 0.91). Significant astrogliosis (7, 14 days and 9 months: p <0.0001) and increased metallothionein (MT I + II) expression (7 and 14 days: p <0.0001; 9 months: p <0.001) were also seen in silver-exposed brain tissue. We conclude that metallic silver implants release silver ions causing neuroinflammation and a progressive tissue loss in the brain.

AB - Silver is a metal with well-known antibacterial effects. This makes silver an attractive coating material for medical devices for use inside the body, e.g. orthopaedic prostheses and catheters used in neurosurgery as it has been found to reduce the high risk of infections. Lately, the use of nano-silver particles in the industry, e.g. woven into fabrics and furniture has increased, and thus the exposure to silver particles in daily life increases. To study the effect of metallic silver particles on nervous tissue, we injected micron-sized silver particles into the mouse brain by stereotactic procedures. After 7, 14 days and 9 months, the silver-exposed animals had considerable brain damage seen as cavity formation and inflammation adjacent to the injected metallic silver particles. The tissue loss involved both cortical and hippocampal structures and resulted in enlargement of the lateral ventricles. Autometallographic silver enhancement showed silver uptake in lysosomes of glia cells and neurons in the ipsilateral cortex and hippocampus alongside a minor uptake on the contralateral side. Silver was also detected in ependymal cells and the choroid plexus. After 9 months, spreading of silver to the kidneys was seen. Cell counts of immunostained sections showed that metallic silver induced a statistically significant inflammatory response, i.e. increased microgliosis (7 days: p <0.0001; 14 days: p <0.01; 9 months: p <0.0001) and TNF-α expression (7 and 14 days: p <0.0001; 9 months: p = 0.91). Significant astrogliosis (7, 14 days and 9 months: p <0.0001) and increased metallothionein (MT I + II) expression (7 and 14 days: p <0.0001; 9 months: p <0.001) were also seen in silver-exposed brain tissue. We conclude that metallic silver implants release silver ions causing neuroinflammation and a progressive tissue loss in the brain.

KW - Animals

KW - Brain

KW - Cell Count

KW - Female

KW - Gene Expression Regulation

KW - Inflammation

KW - Metallothionein

KW - Mice

KW - Mice, Inbred BALB C

KW - Prostheses and Implants

KW - Silver

KW - Stereotaxic Techniques

KW - Time Factors

KW - Tissue Distribution

U2 - 10.1111/j.1742-7843.2010.00668.x

DO - 10.1111/j.1742-7843.2010.00668.x

M3 - Journal article

VL - 109

SP - 1

EP - 10

JO - Basic & Clinical Pharmacology & Toxicology

JF - Basic & Clinical Pharmacology & Toxicology

SN - 1742-7835

IS - 1

ER -