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Bo Barker Jørgensen

Sulfate Transporters in Dissimilatory Sulfate Reducing Microorganisms: A Comparative Genomics Analysis

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Sulfate Transporters in Dissimilatory Sulfate Reducing Microorganisms : A Comparative Genomics Analysis. / Marietou, Angeliki; Roy, Hans; Jorgensen, Bo B.; Kjeldsen, Kasper U.

In: Frontiers in Microbiology, Vol. 9, 309, 02.03.2018.

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@article{8593ba6d14364ffc8f5936d29c70557d,
title = "Sulfate Transporters in Dissimilatory Sulfate Reducing Microorganisms: A Comparative Genomics Analysis",
abstract = "The first step in the sulfate reduction pathway is the transport of sulfate across the cell membrane. This uptake has a major effect on sulfate reduction rates. Much of the information available on sulfate transport was obtained by studies on assimilatory sulfate reduction, where sulfate transporters were identified among several types of protein families. Despite our growing knowledge on the physiology of dissimilatory sulfate-reducing microorganisms (SRM) there are no studies identifying the proteins involved in sulfate uptake in members of this ecologically important group of anaerobes. We surveyed the complete genomes of 44 sulfate-reducing bacteria and archaea across six phyla and identified putative sulfate transporter encoding genes from four out of the five surveyed protein families based on homology. We did not find evidence that ABC-type transporters (SulT) are involved in the uptake of sulfate in SRM. We speculate that members of the CysP sulfate transporters could play a key role in the uptake of sulfate in thermophilic SRM. Putative CysZ-type sulfate transporters were present in all genomes examined suggesting that this overlooked group of sulfate transporters might play a role in sulfate transport in dissimilatory sulfate reducers alongside SulP. Our in silico analysis highlights several targets for further molecular studies in order to understand this key step in the metabolism of SRMs.",
keywords = "sulfate transporter, sulfate-reducing microorganisms, SulP, CysP, CysZ, INDICUS GEN. NOV., ESCHERICHIA-COLI, DESULFOVIBRIO-DESULFURICANS, MYCOBACTERIUM-TUBERCULOSIS, THERMODESULFOBACTERIUM-COMMUNE, CLASSIFICATION DATABASE, SULFITE REDUCTASES, COMPLETE OXIDATION, MARINE-SEDIMENTS, PROTEIN RV1739C",
author = "Angeliki Marietou and Hans Roy and Jorgensen, {Bo B.} and Kjeldsen, {Kasper U.}",
year = "2018",
month = mar,
day = "2",
doi = "10.3389/fmicb.2018.00309",
language = "English",
volume = "9",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - Sulfate Transporters in Dissimilatory Sulfate Reducing Microorganisms

T2 - A Comparative Genomics Analysis

AU - Marietou, Angeliki

AU - Roy, Hans

AU - Jorgensen, Bo B.

AU - Kjeldsen, Kasper U.

PY - 2018/3/2

Y1 - 2018/3/2

N2 - The first step in the sulfate reduction pathway is the transport of sulfate across the cell membrane. This uptake has a major effect on sulfate reduction rates. Much of the information available on sulfate transport was obtained by studies on assimilatory sulfate reduction, where sulfate transporters were identified among several types of protein families. Despite our growing knowledge on the physiology of dissimilatory sulfate-reducing microorganisms (SRM) there are no studies identifying the proteins involved in sulfate uptake in members of this ecologically important group of anaerobes. We surveyed the complete genomes of 44 sulfate-reducing bacteria and archaea across six phyla and identified putative sulfate transporter encoding genes from four out of the five surveyed protein families based on homology. We did not find evidence that ABC-type transporters (SulT) are involved in the uptake of sulfate in SRM. We speculate that members of the CysP sulfate transporters could play a key role in the uptake of sulfate in thermophilic SRM. Putative CysZ-type sulfate transporters were present in all genomes examined suggesting that this overlooked group of sulfate transporters might play a role in sulfate transport in dissimilatory sulfate reducers alongside SulP. Our in silico analysis highlights several targets for further molecular studies in order to understand this key step in the metabolism of SRMs.

AB - The first step in the sulfate reduction pathway is the transport of sulfate across the cell membrane. This uptake has a major effect on sulfate reduction rates. Much of the information available on sulfate transport was obtained by studies on assimilatory sulfate reduction, where sulfate transporters were identified among several types of protein families. Despite our growing knowledge on the physiology of dissimilatory sulfate-reducing microorganisms (SRM) there are no studies identifying the proteins involved in sulfate uptake in members of this ecologically important group of anaerobes. We surveyed the complete genomes of 44 sulfate-reducing bacteria and archaea across six phyla and identified putative sulfate transporter encoding genes from four out of the five surveyed protein families based on homology. We did not find evidence that ABC-type transporters (SulT) are involved in the uptake of sulfate in SRM. We speculate that members of the CysP sulfate transporters could play a key role in the uptake of sulfate in thermophilic SRM. Putative CysZ-type sulfate transporters were present in all genomes examined suggesting that this overlooked group of sulfate transporters might play a role in sulfate transport in dissimilatory sulfate reducers alongside SulP. Our in silico analysis highlights several targets for further molecular studies in order to understand this key step in the metabolism of SRMs.

KW - sulfate transporter

KW - sulfate-reducing microorganisms

KW - SulP

KW - CysP

KW - CysZ

KW - INDICUS GEN. NOV.

KW - ESCHERICHIA-COLI

KW - DESULFOVIBRIO-DESULFURICANS

KW - MYCOBACTERIUM-TUBERCULOSIS

KW - THERMODESULFOBACTERIUM-COMMUNE

KW - CLASSIFICATION DATABASE

KW - SULFITE REDUCTASES

KW - COMPLETE OXIDATION

KW - MARINE-SEDIMENTS

KW - PROTEIN RV1739C

U2 - 10.3389/fmicb.2018.00309

DO - 10.3389/fmicb.2018.00309

M3 - Review

C2 - 29551997

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 309

ER -