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Bo Barker Jørgensen

Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea

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Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea. / Braun, Stefan; Morono, Yuki; Littmann, Sten; Kuypers, Marcel M M; Aslan, Hüsnü; Dong, MD; Jørgensen, Bo Barker; Lomstein, Bente Aagaard.

In: Frontiers in Microbiology, Vol. 7, 1375, 31.08.2016, p. 1-13.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Braun, S, Morono, Y, Littmann, S, Kuypers, MMM, Aslan, H, Dong, MD, Jørgensen, BB & Lomstein, BA 2016, 'Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea', Frontiers in Microbiology, vol. 7, 1375, pp. 1-13. https://doi.org/10.3389/fmicb.2016.01375

APA

Braun, S., Morono, Y., Littmann, S., Kuypers, M. M. M., Aslan, H., Dong, MD., Jørgensen, B. B., & Lomstein, B. A. (2016). Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea. Frontiers in Microbiology, 7, 1-13. [1375]. https://doi.org/10.3389/fmicb.2016.01375

CBE

MLA

Vancouver

Braun S, Morono Y, Littmann S, Kuypers MMM, Aslan H, Dong MD et al. Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea. Frontiers in Microbiology. 2016 Aug 31;7:1-13. 1375. https://doi.org/10.3389/fmicb.2016.01375

Author

Braun, Stefan ; Morono, Yuki ; Littmann, Sten ; Kuypers, Marcel M M ; Aslan, Hüsnü ; Dong, MD ; Jørgensen, Bo Barker ; Lomstein, Bente Aagaard. / Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea. In: Frontiers in Microbiology. 2016 ; Vol. 7. pp. 1-13.

Bibtex

@article{b334d1acd4bf4b1c88707cfe0f0d0075,
title = "Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea",
abstract = "The discovery of a microbial ecosystem in ocean sediments has evoked interest in life under extreme energy limitation and its role in global element cycling. However, fundamental parameters such as the size and the amount of biomass of sub-seafloor microbial cells are poorly constrained. Here we determined the volume and the carbon content of microbial cells from a marine sediment drill core retrieved by the Integrated Ocean Drilling Program (IODP), Expedition 347, at Landsort Deep, Baltic Sea. To determine their shape and volume, cells were separated from the sediment matrix by multi-layer density centrifugation and visualized via epifluorescence microscopy (FM) and scanning electron microscopy (SEM). Total cell-carbon was calculated from amino acid-carbon, which was analyzed by high-performance liquid chromatography (HPLC) after cells had been purified by fluorescence-activated cell sorting (FACS). The majority of microbial cells in the sediment have coccoid or slightly elongated morphology. From the sediment surface to the deepest investigated sample (∼60 m below the seafloor), the cell volume of both coccoid and elongated cells decreased by an order of magnitude from ∼0.05 to 0.005 μm3. The cell-specific carbon content was 19–31 fg C cell−1, which is at the lower end of previous estimates that were used for global estimates of microbial biomass. The cell-specific carbon density increased with sediment depth from about 200 to 1000 fg C μm−3, suggesting that cells decrease their water content and grow small cell sizes as adaptation to the long-term subsistence at very low energy availability in the deep biosphere. We present for the first time depth-related data on the cell volume and carbon content of sedimentary microbial cells buried down to 60 m below the seafloor. Our data enable estimates of volume- and biomass-specific cellular rates of energy metabolism in the deep biosphere and will improve global estimates of microbial biomass.",
keywords = "cell volume, carbon content, carbon density, cell extraction, FACS, IODP, Expedition 347, deep biosphere",
author = "Stefan Braun and Yuki Morono and Sten Littmann and Kuypers, {Marcel M M} and H{\"u}sn{\"u} Aslan and MD Dong and J{\o}rgensen, {Bo Barker} and Lomstein, {Bente Aagaard}",
year = "2016",
month = aug,
day = "31",
doi = "10.3389/fmicb.2016.01375",
language = "English",
volume = "7",
pages = "1--13",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea

AU - Braun, Stefan

AU - Morono, Yuki

AU - Littmann, Sten

AU - Kuypers, Marcel M M

AU - Aslan, Hüsnü

AU - Dong, MD

AU - Jørgensen, Bo Barker

AU - Lomstein, Bente Aagaard

PY - 2016/8/31

Y1 - 2016/8/31

N2 - The discovery of a microbial ecosystem in ocean sediments has evoked interest in life under extreme energy limitation and its role in global element cycling. However, fundamental parameters such as the size and the amount of biomass of sub-seafloor microbial cells are poorly constrained. Here we determined the volume and the carbon content of microbial cells from a marine sediment drill core retrieved by the Integrated Ocean Drilling Program (IODP), Expedition 347, at Landsort Deep, Baltic Sea. To determine their shape and volume, cells were separated from the sediment matrix by multi-layer density centrifugation and visualized via epifluorescence microscopy (FM) and scanning electron microscopy (SEM). Total cell-carbon was calculated from amino acid-carbon, which was analyzed by high-performance liquid chromatography (HPLC) after cells had been purified by fluorescence-activated cell sorting (FACS). The majority of microbial cells in the sediment have coccoid or slightly elongated morphology. From the sediment surface to the deepest investigated sample (∼60 m below the seafloor), the cell volume of both coccoid and elongated cells decreased by an order of magnitude from ∼0.05 to 0.005 μm3. The cell-specific carbon content was 19–31 fg C cell−1, which is at the lower end of previous estimates that were used for global estimates of microbial biomass. The cell-specific carbon density increased with sediment depth from about 200 to 1000 fg C μm−3, suggesting that cells decrease their water content and grow small cell sizes as adaptation to the long-term subsistence at very low energy availability in the deep biosphere. We present for the first time depth-related data on the cell volume and carbon content of sedimentary microbial cells buried down to 60 m below the seafloor. Our data enable estimates of volume- and biomass-specific cellular rates of energy metabolism in the deep biosphere and will improve global estimates of microbial biomass.

AB - The discovery of a microbial ecosystem in ocean sediments has evoked interest in life under extreme energy limitation and its role in global element cycling. However, fundamental parameters such as the size and the amount of biomass of sub-seafloor microbial cells are poorly constrained. Here we determined the volume and the carbon content of microbial cells from a marine sediment drill core retrieved by the Integrated Ocean Drilling Program (IODP), Expedition 347, at Landsort Deep, Baltic Sea. To determine their shape and volume, cells were separated from the sediment matrix by multi-layer density centrifugation and visualized via epifluorescence microscopy (FM) and scanning electron microscopy (SEM). Total cell-carbon was calculated from amino acid-carbon, which was analyzed by high-performance liquid chromatography (HPLC) after cells had been purified by fluorescence-activated cell sorting (FACS). The majority of microbial cells in the sediment have coccoid or slightly elongated morphology. From the sediment surface to the deepest investigated sample (∼60 m below the seafloor), the cell volume of both coccoid and elongated cells decreased by an order of magnitude from ∼0.05 to 0.005 μm3. The cell-specific carbon content was 19–31 fg C cell−1, which is at the lower end of previous estimates that were used for global estimates of microbial biomass. The cell-specific carbon density increased with sediment depth from about 200 to 1000 fg C μm−3, suggesting that cells decrease their water content and grow small cell sizes as adaptation to the long-term subsistence at very low energy availability in the deep biosphere. We present for the first time depth-related data on the cell volume and carbon content of sedimentary microbial cells buried down to 60 m below the seafloor. Our data enable estimates of volume- and biomass-specific cellular rates of energy metabolism in the deep biosphere and will improve global estimates of microbial biomass.

KW - cell volume

KW - carbon content

KW - carbon density

KW - cell extraction

KW - FACS

KW - IODP

KW - Expedition 347

KW - deep biosphere

U2 - 10.3389/fmicb.2016.01375

DO - 10.3389/fmicb.2016.01375

M3 - Journal article

C2 - 27630628

VL - 7

SP - 1

EP - 13

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 1375

ER -