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Bo Barker Jørgensen

Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling

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Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling. / Wasmund, Kenneth; Cooper, Myriel; Schreiber, Lars; Lloyd, Karen G.; Baker, Brett J.; Petersen, Dorthe Groth; Jørgensen, Bo Barker; Stepanauskas, Ramunas; Reinhardt, Richard; Schramm, Andreas; Loy, Alexander; Adrian, Lorenz.

In: mBio (Online), Vol. 7, No. 3, e00266-16, 03.05.2016.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Wasmund, K, Cooper, M, Schreiber, L, Lloyd, KG, Baker, BJ, Petersen, DG, Jørgensen, BB, Stepanauskas, R, Reinhardt, R, Schramm, A, Loy, A & Adrian, L 2016, 'Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling', mBio (Online), vol. 7, no. 3, e00266-16. https://doi.org/10.1128/mBio.00266-16

APA

Wasmund, K., Cooper, M., Schreiber, L., Lloyd, K. G., Baker, B. J., Petersen, D. G., Jørgensen, B. B., Stepanauskas, R., Reinhardt, R., Schramm, A., Loy, A., & Adrian, L. (2016). Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling. mBio (Online), 7(3), [e00266-16]. https://doi.org/10.1128/mBio.00266-16

CBE

Wasmund K, Cooper M, Schreiber L, Lloyd KG, Baker BJ, Petersen DG, Jørgensen BB, Stepanauskas R, Reinhardt R, Schramm A, Loy A, Adrian L. 2016. Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling. mBio (Online). 7(3):Article e00266-16. https://doi.org/10.1128/mBio.00266-16

MLA

Vancouver

Author

Wasmund, Kenneth ; Cooper, Myriel ; Schreiber, Lars ; Lloyd, Karen G. ; Baker, Brett J. ; Petersen, Dorthe Groth ; Jørgensen, Bo Barker ; Stepanauskas, Ramunas ; Reinhardt, Richard ; Schramm, Andreas ; Loy, Alexander ; Adrian, Lorenz. / Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling. In: mBio (Online). 2016 ; Vol. 7, No. 3.

Bibtex

@article{8c92842688d4438783283a13f87e2e22,
title = "Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling",
abstract = "The marine subsurface sediment biosphere is widely inhabited by bacteria affiliated with the class Dehalococcoidia (DEH), phylum Chloroflexi, and yet little is known regarding their metabolisms. In this report, genomic content from a single DEH cell (DEH-C11) with a 16S rRNA gene that was affiliated with a diverse cluster of 16S rRNA gene sequences prevalent in marine sediments was obtained from sediments of Aarhus Bay, Denmark. The distinctive gene content of this cell suggests metabolic characteristics that differ from those of known DEH and Chloroflexi. The presence of genes encoding dissimilatory sulfite reductase (Dsr) suggests that DEH could respire oxidized sulfur compounds, although Chloroflexi have never been implicated in this mode of sulfur cycling. Using long-range PCR assays targeting DEH dsr loci, dsrAB genes were amplified and sequenced from various marine sediments. Many of the amplified dsrAB sequences were affiliated with the DEH Dsr clade, which we propose equates to a family-level clade. This provides supporting evidence for the potential for sulfite reduction by diverse DEH species. DEH-C11 also harbored genes encoding reductases for arsenate, dimethyl sulfoxide, and halogenated organics. The reductive dehalogenase homolog (RdhA) forms a monophyletic clade along with RdhA sequences from various DEH-derived contigs retrieved from available metagenomes. Multiple facts indicate that this RdhA may not be a terminal reductase. The presence of other genes indicated that nutrients and energy may be derived from the oxidation of substituted homocyclic and heterocyclic aromatic compounds. Together, these results suggest that marine DEH play a previously unrecognized role in sulfur cycling and reveal the potential for expanded catabolic and respiratory functions among subsurface DEH.",
author = "Kenneth Wasmund and Myriel Cooper and Lars Schreiber and Lloyd, {Karen G.} and Baker, {Brett J.} and Petersen, {Dorthe Groth} and J{\o}rgensen, {Bo Barker} and Ramunas Stepanauskas and Richard Reinhardt and Andreas Schramm and Alexander Loy and Lorenz Adrian",
year = "2016",
month = may,
day = "3",
doi = "10.1128/mBio.00266-16",
language = "English",
volume = "7",
journal = "mBio (Online)",
issn = "2150-7511",
publisher = "American Society for Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling

AU - Wasmund, Kenneth

AU - Cooper, Myriel

AU - Schreiber, Lars

AU - Lloyd, Karen G.

AU - Baker, Brett J.

AU - Petersen, Dorthe Groth

AU - Jørgensen, Bo Barker

AU - Stepanauskas, Ramunas

AU - Reinhardt, Richard

AU - Schramm, Andreas

AU - Loy, Alexander

AU - Adrian, Lorenz

PY - 2016/5/3

Y1 - 2016/5/3

N2 - The marine subsurface sediment biosphere is widely inhabited by bacteria affiliated with the class Dehalococcoidia (DEH), phylum Chloroflexi, and yet little is known regarding their metabolisms. In this report, genomic content from a single DEH cell (DEH-C11) with a 16S rRNA gene that was affiliated with a diverse cluster of 16S rRNA gene sequences prevalent in marine sediments was obtained from sediments of Aarhus Bay, Denmark. The distinctive gene content of this cell suggests metabolic characteristics that differ from those of known DEH and Chloroflexi. The presence of genes encoding dissimilatory sulfite reductase (Dsr) suggests that DEH could respire oxidized sulfur compounds, although Chloroflexi have never been implicated in this mode of sulfur cycling. Using long-range PCR assays targeting DEH dsr loci, dsrAB genes were amplified and sequenced from various marine sediments. Many of the amplified dsrAB sequences were affiliated with the DEH Dsr clade, which we propose equates to a family-level clade. This provides supporting evidence for the potential for sulfite reduction by diverse DEH species. DEH-C11 also harbored genes encoding reductases for arsenate, dimethyl sulfoxide, and halogenated organics. The reductive dehalogenase homolog (RdhA) forms a monophyletic clade along with RdhA sequences from various DEH-derived contigs retrieved from available metagenomes. Multiple facts indicate that this RdhA may not be a terminal reductase. The presence of other genes indicated that nutrients and energy may be derived from the oxidation of substituted homocyclic and heterocyclic aromatic compounds. Together, these results suggest that marine DEH play a previously unrecognized role in sulfur cycling and reveal the potential for expanded catabolic and respiratory functions among subsurface DEH.

AB - The marine subsurface sediment biosphere is widely inhabited by bacteria affiliated with the class Dehalococcoidia (DEH), phylum Chloroflexi, and yet little is known regarding their metabolisms. In this report, genomic content from a single DEH cell (DEH-C11) with a 16S rRNA gene that was affiliated with a diverse cluster of 16S rRNA gene sequences prevalent in marine sediments was obtained from sediments of Aarhus Bay, Denmark. The distinctive gene content of this cell suggests metabolic characteristics that differ from those of known DEH and Chloroflexi. The presence of genes encoding dissimilatory sulfite reductase (Dsr) suggests that DEH could respire oxidized sulfur compounds, although Chloroflexi have never been implicated in this mode of sulfur cycling. Using long-range PCR assays targeting DEH dsr loci, dsrAB genes were amplified and sequenced from various marine sediments. Many of the amplified dsrAB sequences were affiliated with the DEH Dsr clade, which we propose equates to a family-level clade. This provides supporting evidence for the potential for sulfite reduction by diverse DEH species. DEH-C11 also harbored genes encoding reductases for arsenate, dimethyl sulfoxide, and halogenated organics. The reductive dehalogenase homolog (RdhA) forms a monophyletic clade along with RdhA sequences from various DEH-derived contigs retrieved from available metagenomes. Multiple facts indicate that this RdhA may not be a terminal reductase. The presence of other genes indicated that nutrients and energy may be derived from the oxidation of substituted homocyclic and heterocyclic aromatic compounds. Together, these results suggest that marine DEH play a previously unrecognized role in sulfur cycling and reveal the potential for expanded catabolic and respiratory functions among subsurface DEH.

U2 - 10.1128/mBio.00266-16

DO - 10.1128/mBio.00266-16

M3 - Journal article

C2 - 27143384

VL - 7

JO - mBio (Online)

JF - mBio (Online)

SN - 2150-7511

IS - 3

M1 - e00266-16

ER -