Aarhus University Seal / Aarhus Universitets segl

Bo Barker Jørgensen

Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment

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Standard

Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment. / Mueller, Albert L.; Pelikan, Claus; de Rezende, Julia R.; Wasmund, Kenneth; Putz, Martina; Glombitza, Clemens; Kjeldsen, Kasper U.; Jorgensen, Bo Barker; Loy, Alexander.

In: Environmental Microbiology, Vol. 20, No. 8, 08.2018, p. 2927-2940.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Mueller, AL, Pelikan, C, de Rezende, JR, Wasmund, K, Putz, M, Glombitza, C, Kjeldsen, KU, Jorgensen, BB & Loy, A 2018, 'Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment', Environmental Microbiology, vol. 20, no. 8, pp. 2927-2940. https://doi.org/10.1111/1462-2920.14297

APA

Mueller, A. L., Pelikan, C., de Rezende, J. R., Wasmund, K., Putz, M., Glombitza, C., Kjeldsen, K. U., Jorgensen, B. B., & Loy, A. (2018). Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment. Environmental Microbiology, 20(8), 2927-2940. https://doi.org/10.1111/1462-2920.14297

CBE

Mueller AL, Pelikan C, de Rezende JR, Wasmund K, Putz M, Glombitza C, Kjeldsen KU, Jorgensen BB, Loy A. 2018. Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment. Environmental Microbiology. 20(8):2927-2940. https://doi.org/10.1111/1462-2920.14297

MLA

Vancouver

Mueller AL, Pelikan C, de Rezende JR, Wasmund K, Putz M, Glombitza C et al. Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment. Environmental Microbiology. 2018 Aug;20(8):2927-2940. https://doi.org/10.1111/1462-2920.14297

Author

Mueller, Albert L. ; Pelikan, Claus ; de Rezende, Julia R. ; Wasmund, Kenneth ; Putz, Martina ; Glombitza, Clemens ; Kjeldsen, Kasper U. ; Jorgensen, Bo Barker ; Loy, Alexander. / Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment. In: Environmental Microbiology. 2018 ; Vol. 20, No. 8. pp. 2927-2940.

Bibtex

@article{803b41e69db4490e99ec6abfa192e67f,
title = "Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment",
abstract = "Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies-carbon-flow during anaerobic OM degradation in arctic marine sediment using stable isotope probing. We supplemented sediment incubations with C-13-labeled cyanobacterial necromass (spirulina), mimicking fresh OM input, or acetate, an important OM degradation intermediate and monitored sulfate reduction rates and concentrations of volatile fatty acids (VFAs) during substrate degradation. Sequential 16S rRNA gene and transcript amplicon sequencing and fluorescence in situ hybridization combined with Raman microspectroscopy revealed that only few bacterial species were the main degraders of C-13-spirulina necromass. Psychrilyobacter, Psychromonas, Marinifilum, Colwellia, Marinilabiaceae and Clostridiales species were likely involved in the primary hydrolysis and fermentation of spirulina. VFAs, mainly acetate, produced from spirulina degradation were mineralized by sulfate-reducing bacteria and an Arcobacter species. Cellular activity of Desulfobacteraceae and Desulfobulbaceae species during acetoclastic sulfate reduction was largely decoupled from relative 16S rRNA gene abundance shifts. Our findings provide new insights into the identities and physiological constraints that determine the population dynamics of key microorganisms during complex OM degradation in arctic marine sediments.(c) 2018 Society for Applied Microbiology and John Wiley & Sons Ltd",
keywords = "SULFATE-REDUCING BACTERIA, ORGANIC-MATTER, SP-NOV, MICROBIAL COMMUNITY, EXTRACELLULAR ENZYMES, CARBON DEGRADATION, ELEMENTAL SULFUR, SURFACE SEDIMENT, FJORD SEDIMENTS, SEA BED",
author = "Mueller, {Albert L.} and Claus Pelikan and {de Rezende}, {Julia R.} and Kenneth Wasmund and Martina Putz and Clemens Glombitza and Kjeldsen, {Kasper U.} and Jorgensen, {Bo Barker} and Alexander Loy",
year = "2018",
month = aug,
doi = "10.1111/1462-2920.14297",
language = "English",
volume = "20",
pages = "2927--2940",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "8",

}

RIS

TY - JOUR

T1 - Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment

AU - Mueller, Albert L.

AU - Pelikan, Claus

AU - de Rezende, Julia R.

AU - Wasmund, Kenneth

AU - Putz, Martina

AU - Glombitza, Clemens

AU - Kjeldsen, Kasper U.

AU - Jorgensen, Bo Barker

AU - Loy, Alexander

PY - 2018/8

Y1 - 2018/8

N2 - Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies-carbon-flow during anaerobic OM degradation in arctic marine sediment using stable isotope probing. We supplemented sediment incubations with C-13-labeled cyanobacterial necromass (spirulina), mimicking fresh OM input, or acetate, an important OM degradation intermediate and monitored sulfate reduction rates and concentrations of volatile fatty acids (VFAs) during substrate degradation. Sequential 16S rRNA gene and transcript amplicon sequencing and fluorescence in situ hybridization combined with Raman microspectroscopy revealed that only few bacterial species were the main degraders of C-13-spirulina necromass. Psychrilyobacter, Psychromonas, Marinifilum, Colwellia, Marinilabiaceae and Clostridiales species were likely involved in the primary hydrolysis and fermentation of spirulina. VFAs, mainly acetate, produced from spirulina degradation were mineralized by sulfate-reducing bacteria and an Arcobacter species. Cellular activity of Desulfobacteraceae and Desulfobulbaceae species during acetoclastic sulfate reduction was largely decoupled from relative 16S rRNA gene abundance shifts. Our findings provide new insights into the identities and physiological constraints that determine the population dynamics of key microorganisms during complex OM degradation in arctic marine sediments.(c) 2018 Society for Applied Microbiology and John Wiley & Sons Ltd

AB - Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies-carbon-flow during anaerobic OM degradation in arctic marine sediment using stable isotope probing. We supplemented sediment incubations with C-13-labeled cyanobacterial necromass (spirulina), mimicking fresh OM input, or acetate, an important OM degradation intermediate and monitored sulfate reduction rates and concentrations of volatile fatty acids (VFAs) during substrate degradation. Sequential 16S rRNA gene and transcript amplicon sequencing and fluorescence in situ hybridization combined with Raman microspectroscopy revealed that only few bacterial species were the main degraders of C-13-spirulina necromass. Psychrilyobacter, Psychromonas, Marinifilum, Colwellia, Marinilabiaceae and Clostridiales species were likely involved in the primary hydrolysis and fermentation of spirulina. VFAs, mainly acetate, produced from spirulina degradation were mineralized by sulfate-reducing bacteria and an Arcobacter species. Cellular activity of Desulfobacteraceae and Desulfobulbaceae species during acetoclastic sulfate reduction was largely decoupled from relative 16S rRNA gene abundance shifts. Our findings provide new insights into the identities and physiological constraints that determine the population dynamics of key microorganisms during complex OM degradation in arctic marine sediments.(c) 2018 Society for Applied Microbiology and John Wiley & Sons Ltd

KW - SULFATE-REDUCING BACTERIA

KW - ORGANIC-MATTER

KW - SP-NOV

KW - MICROBIAL COMMUNITY

KW - EXTRACELLULAR ENZYMES

KW - CARBON DEGRADATION

KW - ELEMENTAL SULFUR

KW - SURFACE SEDIMENT

KW - FJORD SEDIMENTS

KW - SEA BED

U2 - 10.1111/1462-2920.14297

DO - 10.1111/1462-2920.14297

M3 - Journal article

C2 - 30051650

VL - 20

SP - 2927

EP - 2940

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 8

ER -