Aarhus University Seal / Aarhus Universitets segl

Birte Boelt

Classification of viable and non-viable spinach (Spinacia oleracea L.) seeds by single seed near infrared spectroscopy and extended canonical variates analysis

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Classification of viable and non-viable spinach (Spinacia oleracea L.) seeds by single seed near infrared spectroscopy and extended canonical variates analysis. / Olesen, Merete Halkjær; Shetty, Nisha; Gislum, René; Boelt, Birte.

In: Journal of Near Infrared Spectroscopy, Vol. 19, No. 3, 27.06.2011, p. 171-180.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{b6ec90d6a4454ec189139e70069c34c3,
title = "Classification of viable and non-viable spinach (Spinacia oleracea L.) seeds by single seed near infrared spectroscopy and extended canonical variates analysis",
abstract = "Near-infrared (NIR) reflectance spectroscopy is a common non-destructive method for predicting seed quality parameters, such as moisture, oil, carbohydrates and protein content. Furthermore, variations in absorbance between germinating and non-germinating seeds have been shown in single seed studies. Spinach (Spinacia oleracea L.) is the major crop in vegetable seed production in Denmark and two seed lots with viability percentages of 90% and 97% were chosen for examination by single seed NIR spectroscopy. Lipids play a major role in both ageing and germination. During accelerated ageing, lipid peroxidation leads to deterioration of cell membranes and contributes in that way to reducing seed viability of the seed sample. These biochemical changes may be the reason for a clear grouping between aged and non-aged seeds when performing the extended canonical variates analysis (ECVA). Assigning the difference of scatter corrected absorbance spectra from aged and non-aged seeds also lead to CH2, CH3 and HC=CH structures, which are some of the functional groups in lipids. In the ECVA plot, there was a clear difference between seeds with and without a pericarp. Evaluating the spectra, the pattern of peaks was almost similar, but the intensity was different in the absorption band at 1350 nm. The number of misclassified seeds ranged from 1.7% to 10.5% and it was lowest in seeds with a pericarp. This indicates the influence of the pericarp during germination, which is in accordance with earlier studies of spinach seeds. Single seed NIR and ECVA classification are potential methods for the prediction of seed viability. ",
keywords = "spinach, seed quality, near infrared spectroscopy, pericarp, accelerated ageing, germination, ECVA, single NIR, spinach, seed quality, near infrared spectroscopy, pericarp, accelerated ageing, germination, ECVA, single NIR",
author = "Olesen, {Merete Halkj{\ae}r} and Nisha Shetty and Ren{\'e} Gislum and Birte Boelt",
year = "2011",
month = jun,
day = "27",
doi = "10.1255/jnirs.928",
language = "English",
volume = "19",
pages = "171--180",
journal = "Journal of Near Infrared Spectroscopy",
issn = "0967-0335",
publisher = "N I R Publications",
number = "3",

}

RIS

TY - JOUR

T1 - Classification of viable and non-viable spinach (Spinacia oleracea L.) seeds by single seed near infrared spectroscopy and extended canonical variates analysis

AU - Olesen, Merete Halkjær

AU - Shetty, Nisha

AU - Gislum, René

AU - Boelt, Birte

PY - 2011/6/27

Y1 - 2011/6/27

N2 - Near-infrared (NIR) reflectance spectroscopy is a common non-destructive method for predicting seed quality parameters, such as moisture, oil, carbohydrates and protein content. Furthermore, variations in absorbance between germinating and non-germinating seeds have been shown in single seed studies. Spinach (Spinacia oleracea L.) is the major crop in vegetable seed production in Denmark and two seed lots with viability percentages of 90% and 97% were chosen for examination by single seed NIR spectroscopy. Lipids play a major role in both ageing and germination. During accelerated ageing, lipid peroxidation leads to deterioration of cell membranes and contributes in that way to reducing seed viability of the seed sample. These biochemical changes may be the reason for a clear grouping between aged and non-aged seeds when performing the extended canonical variates analysis (ECVA). Assigning the difference of scatter corrected absorbance spectra from aged and non-aged seeds also lead to CH2, CH3 and HC=CH structures, which are some of the functional groups in lipids. In the ECVA plot, there was a clear difference between seeds with and without a pericarp. Evaluating the spectra, the pattern of peaks was almost similar, but the intensity was different in the absorption band at 1350 nm. The number of misclassified seeds ranged from 1.7% to 10.5% and it was lowest in seeds with a pericarp. This indicates the influence of the pericarp during germination, which is in accordance with earlier studies of spinach seeds. Single seed NIR and ECVA classification are potential methods for the prediction of seed viability.

AB - Near-infrared (NIR) reflectance spectroscopy is a common non-destructive method for predicting seed quality parameters, such as moisture, oil, carbohydrates and protein content. Furthermore, variations in absorbance between germinating and non-germinating seeds have been shown in single seed studies. Spinach (Spinacia oleracea L.) is the major crop in vegetable seed production in Denmark and two seed lots with viability percentages of 90% and 97% were chosen for examination by single seed NIR spectroscopy. Lipids play a major role in both ageing and germination. During accelerated ageing, lipid peroxidation leads to deterioration of cell membranes and contributes in that way to reducing seed viability of the seed sample. These biochemical changes may be the reason for a clear grouping between aged and non-aged seeds when performing the extended canonical variates analysis (ECVA). Assigning the difference of scatter corrected absorbance spectra from aged and non-aged seeds also lead to CH2, CH3 and HC=CH structures, which are some of the functional groups in lipids. In the ECVA plot, there was a clear difference between seeds with and without a pericarp. Evaluating the spectra, the pattern of peaks was almost similar, but the intensity was different in the absorption band at 1350 nm. The number of misclassified seeds ranged from 1.7% to 10.5% and it was lowest in seeds with a pericarp. This indicates the influence of the pericarp during germination, which is in accordance with earlier studies of spinach seeds. Single seed NIR and ECVA classification are potential methods for the prediction of seed viability.

KW - spinach

KW - seed quality

KW - near infrared spectroscopy

KW - pericarp

KW - accelerated ageing

KW - germination

KW - ECVA

KW - single NIR

KW - spinach

KW - seed quality

KW - near infrared spectroscopy

KW - pericarp

KW - accelerated ageing

KW - germination

KW - ECVA

KW - single NIR

U2 - 10.1255/jnirs.928

DO - 10.1255/jnirs.928

M3 - Journal article

VL - 19

SP - 171

EP - 180

JO - Journal of Near Infrared Spectroscopy

JF - Journal of Near Infrared Spectroscopy

SN - 0967-0335

IS - 3

ER -