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Anni Hangaard Andersen

Drug Stimulated DNA Cleavage Mediated by Cauliflower Topoisomerase II

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  • B S Sørensen, Department of Molecular Biology and Plant Physiology, University of Aarhus, DK-8000 Arhus C, Denmark., Denmark
  • H Fukata, Kobe University, Japan
  • P S Jensen, Department of Molecular Biology and Plant Physiology, University of Aarhus, DK-8000 Arhus C, Denmark., Denmark
  • A H Andersen
  • K Christiansen
  • ,
  • H Fukasawa, Kobe University, Japan
  • O Westergaard

We have investigated cauliflower (Brassica oleracea) topoisomerase II with respect to its interaction with DNA and demonstrate that the enzyme shares the characteristics of topoisomerase II purified from a variety of phylogenetically remote organisms. In the presence of the 2-nitroimidazole Ro 15-0216, cauliflower topoisomerase II-mediated DNA cleavage is extensively stimulated (approximately 20-fold) only at a site recognized as a major cleavage site for the enzyme in the absence of drug. The conservation of the enzyme's DNA specificity in the presence of Ro 15-0216 is in contrast to the effect exerted by traditional topoisomerase II inhibitors, which cause enzyme-mediated cleavage to take place at a multiple number of DNA sites. Ro 15-0216 may therefore prove useful as a tool in the elucidation of the enzyme's DNA interaction sites and its involvement in nucleic acid metabolism in plant cells.

Original languageEnglish
JournalPlant Physiology
Volume95
Issue2
Pages (from-to)659-62
Number of pages4
ISSN0032-0889
Publication statusPublished - Feb 1991

    Research areas

  • Journal Article

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