Anne Gitte Rasmussen Loft

Quantification and phenotyping of serum cholinesterase by enzyme antigen immunoassay: methodological aspects and clinical applicability

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • J Hangaard, Department of Clinical Chemistry, Sønderborg Hospital, Denmark.
  • ,
  • M Whittaker
  • ,
  • A G Loft
  • B Nørgaard-Pedersen

An enzyme antigen immunoassay for a specific determination of serum cholinesterase is described. Polyclonal and monoclonal antibodies against cholinesterase have been used. Hydrophobic binding of the specific antibody to a microtitre plate was followed by incubation with the samples, and the activity of the bound cholinesterase was assayed by the Ellman method. The procedure has been optimized and characterized, with respect to antigen specificity, and the applicability of the assay for cholinesterase phenotyping is demonstrated. The cholinesterase activities, dibucaine-, scoline-, fluoride- and urea numbers were comparable with established reference values. The high sensitivity and specificity of the assay has been used for determination of cholinesterase in amniotic and cerebrospinal fluids, and its applicability in clinical medicine is indicated.

Original languageEnglish
Book seriesScandinavian Journal of Clinical and Laboratory Investigation
Pages (from-to)349-58
Number of pages10
Publication statusPublished - Jun 1991

    Research areas

  • Acetylcholinesterase/blood, Amniotic Fluid/enzymology, Antigens, Cholinesterases/blood, Dithionitrobenzoic Acid, Evaluation Studies as Topic, Female, Humans, Immunoenzyme Techniques, Neural Tube Defects/diagnosis, Phenotype, Pregnancy, Prenatal Diagnosis, Substrate Specificity

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