Anne Gitte Rasmussen Loft

Normal human serum contains two forms of acetylcholinesterase

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We have produced antibodies (polyclonal and monoclonal) against acetylcholinesterase, and used them for immuno-chemical demonstration and quantification of the enzyme in serum. The concentration was 1.2 IU/l. The antibodies were shown not to cross-react with human butyrylcholinesterase, using pure preparations of the enzymes. The serum acetylcholinesterase could be purified using affinity chromatography. The resulting preparation was analyzed using sucrose density gradient centrifugation. Two forms of the enzyme with sedimentation constants of 10.9S and 7.6S were observed, both reacted equally well with monoclonal antibodies towards acetylcholinesterase indicating a common epitope.

Original languageEnglish
JournalClinica chimica acta; international journal of clinical chemistry
Volume158
Issue1
Pages (from-to)1-6
Number of pages6
ISSN0009-8981
Publication statusPublished - 15 Jul 1986

    Research areas

  • Acetylcholinesterase/blood, Adult, Antibody Specificity, Butyrylcholinesterase/blood, Centrifugation, Density Gradient, Humans, Immunoenzyme Techniques

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