Anne Gitte Rasmussen Loft

Immunochemical determination of acetylcholinesterase in amniotic fluid--an evaluation of eleven monoclonal antibodies

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • A G Rasmussen
  • K Sorensen
  • ,
  • J Selmer
  • ,
  • J Zeuthen
  • ,
  • O J Bjerrum
  • ,
  • U Brodbeck
  • ,
  • B Norgaard-Pedersen

Eleven monoclonal antibodies and a polyclonal rabbit antiserum were evaluated with respect to reactivity with acetylcholinesterase (AChE, EC 3.1.1.7) from erythrocytes and brain. Employing our enzyme antigen immunoassay for AChE five selected antibodies were evaluated with regard to their clinical usefulness in the prenatal diagnosis of neural tube defects (NTD). Of these, one antibody preferentially bound the enzyme from human brain, and discerned better than the others pathological samples (anencephaly, spina bifida and encephalocele) from normal ones. With this antibody no false positive values were obtained even if amniotic fluid samples were blood contaminated.

Original languageEnglish
JournalClinica chimica acta; international journal of clinical chemistry
Volume166
Issue1
Pages (from-to)17-25
Number of pages9
ISSN0009-8981
Publication statusPublished - 30 Jun 1987

    Research areas

  • Acetylcholinesterase/analysis, Amniotic Fluid/enzymology, Antibodies, Monoclonal, Antibody Specificity, Brain/enzymology, Erythrocytes/enzymology, Female, Humans, Immunoenzyme Techniques, Neural Tube Defects/diagnosis, Pregnancy, Prenatal Diagnosis

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