Anne Gitte Rasmussen Loft

Alpha-fetoprotein and acetylcholinesterase activity in first- and early second-trimester amniotic fluid

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • F S Jørgensen, Department of Obstetrics and Gynaecology, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, DK-2100 Copenhagen East, Denmark.
  • ,
  • K Sundberg
  • ,
  • A G Loft
  • J Arends
  • ,
  • B Nørgaard-Pedersen

Normal ranges of amniotic fluid alpha-fetoprotein (AFP) and acetylcholinesterase activity (AChE) are described for gestational weeks 11-14 using rocket gel immunoelectrophoresis for AFP quantitation and a monoclonal antibody (4F19) enzyme antigen immunoassay for AChE activity measurement. The normal ranges were established by the examination of 281 amniotic fluid samples from 281 normal pregnancies. AFP was found to increase from a median level of 14.0 MIU/l at 11 weeks to a maximum at 13 weeks (median = 18.0 MIU/l) (P < 0.05), thereafter falling (not significant). No AChE test result exceeded 4.8 nkat/l. In addition, AFP and AChE values for three cases of fetal malformation, identified by the biochemical analyses of amniotic fluid, are given. These cases included two fetuses with a neural tube defect and one fetus with an abdominal wall defect. Amniocentesis was performed at 10, 11, and 14 weeks, respectively. The AFP and AChE values were all high.

Original languageEnglish
JournalPrenatal Diagnosis
Pages (from-to)621-5
Number of pages5
Publication statusPublished - Jul 1995

    Research areas

  • Abdominal Muscles/abnormalities, Acetylcholinesterase/analysis, Adult, Amniocentesis, Amniotic Fluid/chemistry, Antibodies, Monoclonal/analysis, Congenital Abnormalities/diagnosis, Female, Humans, Immunoelectrophoresis, Neural Tube Defects/diagnosis, Pregnancy, Pregnancy Trimester, First, Pregnancy Trimester, Second, Prenatal Diagnosis, alpha-Fetoproteins/analysis

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