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Andreas Bøggild

Mutational analysis of the yeast RNA helicase Sub2p reveals conserved domains required for growth, mRNA export, and genomic stability

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  • Cyril Saguez, Denmark
  • Fernando Alexis Gonzales Zubiate
  • ,
  • Manfred Schmid
  • Andreas Bøggild
  • Chrysa M Latrick, Denmark
  • Francisco Malagon, Denmark
  • Andrea Putnam, Case Western Reserve University, Cleveland, Ohio, United States
  • Lee Sanderson, Denmark
  • Eckhard Jankowsky, Case Western Reserve University, Cleveland, Ohio, United States
  • Ditlev E. Brodersen
  • Torben Heick Jensen
Sub2p/UAP56 is a highly conserved DEAD-box RNA helicase involved in the packaging and nuclear export of mRNA/protein particles (mRNPs). In Saccharomyces cerevisiae, Sub2p is recruited to active chromatin by the pentameric THO complex and incorporated into the larger transcription-export (TREX) complex. Sub2p also plays a role in the maintenance of genome integrity as its inactivation causes severe transcription-dependent recombination of DNA. Despite the central role of Sub2p in early mRNP biology, little is known about its function. Here, we report the presence of an N-terminal motif (NTM) conserved specifically in the Sub2p branch of RNA helicases. Mutation of the NTM causes nuclear accumulation of poly(A)(+) RNA and impaired growth without affecting core helicase functions. Thus, the NTM functions as an autonomous unit. Moreover, two sub2 mutants, that are deficient in ATP binding, act in a trans-dominant negative fashion for growth and induce high recombination rates in vivo. Although wild-type Sub2p is prevented access to transcribed loci in such a background, this does not mechanistically explain the phenotype.
Original languageEnglish
Pages (from-to)1363-1371
Number of pages9
Publication statusPublished - 20 Aug 2013

    Research areas

  • RNA, RNA Export, RNA helicase, genome stability

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