Aage Kristian Olsen Alstrup

Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG

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  • Pia Afzelius, Department of Diagnostic Imaging, North Zealand Hospital, Hillerød, University Hospital of Copenhagen Copenhagen, Denmark.
  • ,
  • Aage K.O. Alstrup
  • Henrik C Schønheyder, Department of Clinical Microbiology, Aalborg University HospitalAalborg, Denmark; Department of Clinical Medicine, Aalborg UniversityAalborg, Denmark.
  • ,
  • Per Borghammer
  • Svend B Jensen, Department of Nuclear Medicine, Aalborg University HospitalAalborg, Denmark; Department of Chemistry and Biochemistry, Aalborg UniversityAalborg, Denmark.
  • ,
  • Dirk Bender
  • Ole L Nielsen, Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.

The aim of this study was to compare (11)C-methionine and (11)C-donepezil positron emission tomography (PET) with (111)In-labeled leukocyte and (99m) Tc-DPD (Tc-99m 3,3-diphosphono-1,2-propanedicarboxylic acid) single-photon emission computed tomography (SPECT), and (18)F-fluorodeoxyglucose ((18)F-FDG) PET to improve detection of osteomyelitis. The tracers' diagnostic utility where tested in a juvenile porcine hematogenously induced osteomyelitis model comparable to osteomyelitis in children. Five 8-9 weeks old female domestic pigs were scanned seven days after intra-arterial inoculation in the right femoral artery with a porcine strain of Staphylococcus aureus. The sequential scan protocol included Computed Tomography, (11)C-methionine and (11)C-donepezil PET, (99m) Tc-DPD and (111)In-labelled leukocytes scintigraphy, and (18)F-FDG PET. This was followed by necropsy of the pigs and gross pathology, histopathology, and microbial examination. The pigs developed a total of 24 osteomyelitic lesions, 4 lesions characterized as contiguous abscesses and pulmonary abscesses (in two pigs). By comparing the 24 osteomyelitic lesions, (18)F-FDG accumulated in 100%, (111)In-leukocytes in 79%, (11)C-methionine in 79%, (11)C-donepezil in 58%, and (99m) Tc-DPD in none. Overall, (18)F-FDG PET was superior to (111)In-leukocyte SPECT and (11)C-methionine in marking infectious lesions.

Original languageEnglish
JournalAmerican Journal of Nuclear Medicine and Molecular Imaging
Pages (from-to)286-300
Number of pages15
Publication statusPublished - 2016

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