Aage Kristian Olsen Alstrup

ISSLS Prize Winner: Positron Emission Tomography and Magnetic Resonance Imaging for Monitoring Interbody Fusion With Equine Bone Protein Extract, Recombinant Human Bone Morphogenetic Protein-2, and Autograft.

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ISSLS Prize Winner: Positron Emission Tomography and Magnetic Resonance Imaging for Monitoring Interbody Fusion With Equine Bone Protein Extract, Recombinant Human Bone Morphogenetic Protein-2, and Autograft. / Foldager, Casper; Bendtsen, Michael; Zou, Xuenong; Zou, Lijin; Olsen, Aage; Munk, Ole; Stødkilde-Jørgensen, Hans; Bünger, Cody.

In: Spine, 2008.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{f2058670b6e411dd99a9000ea68e967b,
title = "ISSLS Prize Winner: Positron Emission Tomography and Magnetic Resonance Imaging for Monitoring Interbody Fusion With Equine Bone Protein Extract, Recombinant Human Bone Morphogenetic Protein-2, and Autograft.",
abstract = "STUDY DESIGN.: Prospective and randomized experimental study with anterior lumbar interbody fusion in a porcine model. OBJECTIVE.: To assess the early time-course of spinal fusion with equine bone protein extract (COLLOSS E), recombinant human bone morphogenetic protein-2 (rhBMP-2), and autograft using quantitative methods of positron emission tomography (PET)/computed tomography and magnetic resonance imaging (MRI). SUMMARY OF BACKGROUND DATA.: Different growth and differentiation factors are currently being used for inducing bone formation in spinal fusion. However, the mechanisms and time-course of bone formation using these graft substitutes remain less known. METHODS.: Eighteen female Danish landrace pigs underwent a 3-level anterior lumbar interbody fusion procedure from L3-L6. A PEEK cage, packed with COLLOSS E, rhBMP-2, or autograft, was randomly placed. Each group of 6 pigs was observed for 2, 4, or 8 weeks, respectively. F PET/computed tomography and MRI examinations were performed, and data were correlated with histomorphometry. PET data were analyzed using a Gjedde-Patlak plot. K-values from the plot correspond to the metabolic rate. T2-values were calculated by T2 mapping. RESULTS.: rhBMP-2 presented the highest bone formation on histologic sections at 25.6{\%} at 4 weeks after surgery. Eight weeks after surgery, autograft had the highest bone formation with 37.3{\%}, which was significantly higher than rhBMP-2 at 30.5{\%} (P < 0.05), and higher than COLLOSS E at 27.0{\%} (P = 0.06). COLLOSS E and rhBMP-2 had significantly higher K-values than autograft (P < 0.05) at 2 weeks after surgery. There were no differences in K-values between COLLOSS E and autograft at 4 and 8 weeks. However, rhBMP-2 was significantly higher at 4 weeks and lower at 8 weeks than these 2 (P < 0.05). Linear correlation, R = 0.8275, was observed for intertrabecular volume/total volume and T2-values. CONCLUSION.: PET and MRI are valid tools for monitoring the process of interbody fusion in vivo. Osteogenic mechanisms using COLLOSS E resembles that of autograft by the process of endochondral ossification. rhBMP-2 deposits osteoid directly on the collagen network.",
keywords = "MRI, MRI, Positron-Emission Tomography",
author = "Casper Foldager and Michael Bendtsen and Xuenong Zou and Lijin Zou and Aage Olsen and Ole Munk and Hans St{\o}dkilde-J{\o}rgensen and Cody B{\"u}nger",
year = "2008",
doi = "10.1097/BRS.0b013e31817fce91",
language = "English",
journal = "Spine",
issn = "0362-2436",
publisher = "LIPPINCOTT WILLIAMS & WILKINS",

}

RIS

TY - JOUR

T1 - ISSLS Prize Winner: Positron Emission Tomography and Magnetic Resonance Imaging for Monitoring Interbody Fusion With Equine Bone Protein Extract, Recombinant Human Bone Morphogenetic Protein-2, and Autograft.

AU - Foldager, Casper

AU - Bendtsen, Michael

AU - Zou, Xuenong

AU - Zou, Lijin

AU - Olsen, Aage

AU - Munk, Ole

AU - Stødkilde-Jørgensen, Hans

AU - Bünger, Cody

PY - 2008

Y1 - 2008

N2 - STUDY DESIGN.: Prospective and randomized experimental study with anterior lumbar interbody fusion in a porcine model. OBJECTIVE.: To assess the early time-course of spinal fusion with equine bone protein extract (COLLOSS E), recombinant human bone morphogenetic protein-2 (rhBMP-2), and autograft using quantitative methods of positron emission tomography (PET)/computed tomography and magnetic resonance imaging (MRI). SUMMARY OF BACKGROUND DATA.: Different growth and differentiation factors are currently being used for inducing bone formation in spinal fusion. However, the mechanisms and time-course of bone formation using these graft substitutes remain less known. METHODS.: Eighteen female Danish landrace pigs underwent a 3-level anterior lumbar interbody fusion procedure from L3-L6. A PEEK cage, packed with COLLOSS E, rhBMP-2, or autograft, was randomly placed. Each group of 6 pigs was observed for 2, 4, or 8 weeks, respectively. F PET/computed tomography and MRI examinations were performed, and data were correlated with histomorphometry. PET data were analyzed using a Gjedde-Patlak plot. K-values from the plot correspond to the metabolic rate. T2-values were calculated by T2 mapping. RESULTS.: rhBMP-2 presented the highest bone formation on histologic sections at 25.6% at 4 weeks after surgery. Eight weeks after surgery, autograft had the highest bone formation with 37.3%, which was significantly higher than rhBMP-2 at 30.5% (P < 0.05), and higher than COLLOSS E at 27.0% (P = 0.06). COLLOSS E and rhBMP-2 had significantly higher K-values than autograft (P < 0.05) at 2 weeks after surgery. There were no differences in K-values between COLLOSS E and autograft at 4 and 8 weeks. However, rhBMP-2 was significantly higher at 4 weeks and lower at 8 weeks than these 2 (P < 0.05). Linear correlation, R = 0.8275, was observed for intertrabecular volume/total volume and T2-values. CONCLUSION.: PET and MRI are valid tools for monitoring the process of interbody fusion in vivo. Osteogenic mechanisms using COLLOSS E resembles that of autograft by the process of endochondral ossification. rhBMP-2 deposits osteoid directly on the collagen network.

AB - STUDY DESIGN.: Prospective and randomized experimental study with anterior lumbar interbody fusion in a porcine model. OBJECTIVE.: To assess the early time-course of spinal fusion with equine bone protein extract (COLLOSS E), recombinant human bone morphogenetic protein-2 (rhBMP-2), and autograft using quantitative methods of positron emission tomography (PET)/computed tomography and magnetic resonance imaging (MRI). SUMMARY OF BACKGROUND DATA.: Different growth and differentiation factors are currently being used for inducing bone formation in spinal fusion. However, the mechanisms and time-course of bone formation using these graft substitutes remain less known. METHODS.: Eighteen female Danish landrace pigs underwent a 3-level anterior lumbar interbody fusion procedure from L3-L6. A PEEK cage, packed with COLLOSS E, rhBMP-2, or autograft, was randomly placed. Each group of 6 pigs was observed for 2, 4, or 8 weeks, respectively. F PET/computed tomography and MRI examinations were performed, and data were correlated with histomorphometry. PET data were analyzed using a Gjedde-Patlak plot. K-values from the plot correspond to the metabolic rate. T2-values were calculated by T2 mapping. RESULTS.: rhBMP-2 presented the highest bone formation on histologic sections at 25.6% at 4 weeks after surgery. Eight weeks after surgery, autograft had the highest bone formation with 37.3%, which was significantly higher than rhBMP-2 at 30.5% (P < 0.05), and higher than COLLOSS E at 27.0% (P = 0.06). COLLOSS E and rhBMP-2 had significantly higher K-values than autograft (P < 0.05) at 2 weeks after surgery. There were no differences in K-values between COLLOSS E and autograft at 4 and 8 weeks. However, rhBMP-2 was significantly higher at 4 weeks and lower at 8 weeks than these 2 (P < 0.05). Linear correlation, R = 0.8275, was observed for intertrabecular volume/total volume and T2-values. CONCLUSION.: PET and MRI are valid tools for monitoring the process of interbody fusion in vivo. Osteogenic mechanisms using COLLOSS E resembles that of autograft by the process of endochondral ossification. rhBMP-2 deposits osteoid directly on the collagen network.

KW - MRI

KW - MRI

KW - Positron-Emission Tomography

U2 - 10.1097/BRS.0b013e31817fce91

DO - 10.1097/BRS.0b013e31817fce91

M3 - Journal article

C2 - 19002076

JO - Spine

JF - Spine

SN - 0362-2436

ER -