Aage Kristian Olsen Alstrup

Comparison of autologous 111In-leukocytes, 18F-FDG, 11C-methionine, 11C-PK11195 and 68Ga-citrate for diagnostic nuclear imaging in a juvenile porcine haematogenous Staphylococcus aureus osteomyelitis model

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  • Ole Lehberg Nielsen, Denmark
  • Pia Afzelius, Denmark
  • Dirk Bender, PET Center, Aarhus University Hospital, Aarhus, Denmark
  • Henrik Carl Schønheyder, Klinisk Mikrobiologi
  • ,
  • Páll S. Leifsson, Sektion, Experimental Animal Models
  • ,
  • Karin Michaelsen Nielsen, Det Sundhedsvidenskabelige Fakultet
  • ,
  • Jytte Overgaard Larsen, Institut for Neurovidenskab og Farmakologi, Københavns Universitet, Denmark
  • Svend Borup Jensen, Aalborg Sygehus, Denmark
  • Aage Kristian Olsen Alstrup
The aim of this study was to compare 111In-labeled leukocyte single-photon emission computed tomography (SPECT) and 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) to PET with tracers that potentially could improve detection of osteomyelitis. We chose 11C-methionine, 11C-PK11195 and 68Ga-citrate and validated their diagnostic utility in a porcine haematogenous osteomyelitis model. Four juvenile 14-15 weeks old female pigs were scanned seven days after intra-arterial inoculation in the right femoral artery with a porcine strain of Staphylococcus aureus using a sequential scan protocol with 18F-FDG, 68Ga-citrate, 11C-methionine, 11C-PK11195, 99mTc-Nanocoll and 111In-labelled autologous leukocytes. This was followed by necropsy of the pigs and gross pathology, histopathology and microbial examination. The pigs developed a total of five osteomyelitis lesions, five lesions characterized as abscesses/cellulitis, arthritis in three joints and five enlarged lymph nodes. None of the tracers accumulated in joints with arthritis. By comparing the 10 infectious lesions, 18F-FDG accumulated in nine, 111In-leukocytes in eight, 11C-methionine in six, 68Ga-citrate in four and 11C-PK11195 accumulated in only one lesion. Overall, 18F-FDG PET was superior to 111In-leukocyte SPECT in marking infectious and proliferative, i.e. hyperplastic, lesions. However, leukocyte SPECT was performed as early scans, approximately 6 h after injection of the leukocytes, to match the requirements of the 18 h long scan protocol. 11C-methionine and possibly 68Ga-citrate may be useful for diagnosis of soft issue lesions.
Original languageEnglish
JournalAmerican Journal of Nuclear Medicine and Molecular Imaging
Volume5
Issue2
Pages (from-to)169-182
ISSN2160-8407
Publication statusPublished - Jan 2015

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