Aage Kristian Olsen Alstrup

A volumetric screening procedure for the Göttingen minipig brain.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • J Jelsing, Denmark
  • Aage K. Olsen
  • P Cumming, Denmark
  • A Gjedde
  • A K Hansen, Denmark
  • S Arnfred, Denmark
  • R Hemmingsen, Denmark
  • Bente Pakkenberg, Denmark
  • Center for Functionally Integrative Neuroscience
  • Positron Emission Tomography Center
A screening procedure was developed to provide quantitative estimates of structural parameters, regional volumes and neuron number, in a neurotoxicologic study of the Göttingen minipig brain. The study material consisted of normal controls and brains collected from young minipigs which had been exposed in utero to the mitotic inhibitor methylazoxymethanol acetate (MAM). Based on stereological principles and systematic sampling techniques, volumetric data from pre-selected regions of the pig brain was obtained using Cavalieri's principles and point-counting. Secondarily, estimates of total hemispheric neocortical cell numbers were obtained from pre-selected groups to test the potential effect of MAM on neuron number. No significant differences were observed in volume of the pre-selected regions of MAM intoxicated pigs nor in estimates of total neocortical neuron number.
Original languageEnglish
JournalExperimental Brain Research
Volume162
Issue4
Pages (from-to)428-35
Number of pages7
ISSN0014-4819
DOIs
Publication statusPublished - 2005

    Research areas

  • Algorithms, Anatomy, Cross-Sectional, Animals, Brain, Cell Count, Disease Models, Animal, Female, Methylazoxymethanol Acetate, Nervous System Malformations, Pilot Projects, Pregnancy, Prenatal Exposure Delayed Effects, Swine, Swine, Miniature, Teratogens

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ID: 11266164