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The Spatial Rotator

Activity: Talk or presentation typesLecture and oral contribution

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Allan Rasmusson - Invited speaker

For thick sections (≥ 25μm) it has previously been suggested to take advantage
of all the available information about entire cells located within the section when
estimating quantitative properties. The optical rotator used test lines in mul-
tiple focal planes and while illustrating the usefulness of the extra information,
it did, however, prove to be slow and cumbersome in practice.
Presented in this paper is a newly developed probe for estimating particle
volume which also exploits all the available 3D information about cells in thick
sections but which in practical situations is of speed and simplicity comparable
to existing and widely used probes, e.g. the rotator and the nucleator. The the-
oretical properties of the developed probe have been verified using simulations
and extensive numerical analysis in 3D, and show that the developed probe is
far superior to a traditional probe utilizing only a single 2D section.
A very promizing feature about the probe is that is does not require isotropic
or vertical sections. This makes it very appealing to use the probe on existing
tissue sections which was prepared without this in mind.
22 Oct 2011

Event (Conference)

Title13th International Conference on Stereology
Abbreviated titleICS13
LocationTsinghua University


  • The spatial rotator

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

ID: 44328675