TY - JOUR

T1 - Yogurt Benefits Bone Mineralization in Ovariectomized Rats with Concomitant Modulation of the Gut Microbiome

AU - He, Weiwei

AU - Xie, Zhuqing

AU - Wittig, Nina Kølln

AU - Zachariassen, Line F

AU - Andersen, Amanda

AU - Andersen, Henrik J

AU - Birkedal, Henrik

AU - Nielsen, Dennis S

AU - Kornerup Hansen, Axel

AU - Bertram, Hanne Christine

PY - 2022/10

Y1 - 2022/10

N2 - SCOPE: Evidence supports that gut-modulating foods potentially can supress bone loss in postmenopausal women. This study aimed to investigate the effect of milk calcium-enriched milk, yogurt, and yogurt-inulin combination on the gut-bone association.METHODS AND RESULTS: A 6-week intervention study was conducted in ovariectomized rats. Four pastes containing milk calcium-fortified milk (M-Ca), milk calcium-fortified yogurt (Y-Ca), inulin-fortified Y-Ca (Y-I-Ca), or an isoconcentration of calcium carbonate (Ca-N), and a calcium-deficient paste were provided. M-Ca did not influence bone mineral density and content (BMD and BMC), femur mechanical strength, or femoral microstructure compared to Ca-N, but Y-Ca increased spine BMD. The serum metabolome revealed that Y-Ca modulated glycine-related pathways with reduced glycine, serine and threonine. No additive effects of yogurt and inulin were found on bone parameters. Correlation analysis showed that increased lactobacilli and reduced Clostridiaceae members in Y-Ca was associated with an increased spine BMD. Increases in Bifidobacterium pseudolongum, Turicibacter, Blautia, and Allobaculum and gut short-chain fatty acids in Y-I-Ca were not reflected in bone parameters.CONCLUSION: Yogurt as calcium vehicle contributed to increased spine BMD concomitant with changes in the gut microbiome and glycine-related pathways, while adding inulin to yogurt did not affect bone mineralization in ovariectomized rats. This article is protected by copyright. All rights reserved.

AB - SCOPE: Evidence supports that gut-modulating foods potentially can supress bone loss in postmenopausal women. This study aimed to investigate the effect of milk calcium-enriched milk, yogurt, and yogurt-inulin combination on the gut-bone association.METHODS AND RESULTS: A 6-week intervention study was conducted in ovariectomized rats. Four pastes containing milk calcium-fortified milk (M-Ca), milk calcium-fortified yogurt (Y-Ca), inulin-fortified Y-Ca (Y-I-Ca), or an isoconcentration of calcium carbonate (Ca-N), and a calcium-deficient paste were provided. M-Ca did not influence bone mineral density and content (BMD and BMC), femur mechanical strength, or femoral microstructure compared to Ca-N, but Y-Ca increased spine BMD. The serum metabolome revealed that Y-Ca modulated glycine-related pathways with reduced glycine, serine and threonine. No additive effects of yogurt and inulin were found on bone parameters. Correlation analysis showed that increased lactobacilli and reduced Clostridiaceae members in Y-Ca was associated with an increased spine BMD. Increases in Bifidobacterium pseudolongum, Turicibacter, Blautia, and Allobaculum and gut short-chain fatty acids in Y-I-Ca were not reflected in bone parameters.CONCLUSION: Yogurt as calcium vehicle contributed to increased spine BMD concomitant with changes in the gut microbiome and glycine-related pathways, while adding inulin to yogurt did not affect bone mineralization in ovariectomized rats. This article is protected by copyright. All rights reserved.

KW - NMR metabolomics

KW - fermented dairy

KW - gut metabolome

KW - gut–bone axis

KW - inulin

UR - http://www.scopus.com/inward/record.url?scp=85138008534&partnerID=8YFLogxK

U2 - 10.1002/mnfr.202200174

DO - 10.1002/mnfr.202200174

M3 - Journal article

C2 - 36039478

VL - 66

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 20

M1 - 2200174

ER -