Whole Grain Consumption Increases Gastrointestinal Content of Sulfate-Conjugated Oxylipins in Pigs − A Multicompartmental Metabolomics Study

TY - JOUR

T1 - Whole Grain Consumption Increases Gastrointestinal Content of Sulfate-Conjugated Oxylipins in Pigs − A Multicompartmental Metabolomics Study

AU - Ingerslev, Anne Krog

AU - Karaman, İbrahim

AU - Bağcıoğlu, Murat

AU - Kohler, Achim

AU - Theil, Peter Kappel

AU - Knudsen, Knud Erik Bach

AU - Hedemann, Mette Skou

PY - 2015/8/7

Y1 - 2015/8/7

N2 - The effects of increased intake of dietary fiber as either arabinoxylan (AX) or resistant starch (RS) compared to a typical low dietary fiber Western-style diet (WSD) on the metabolomics responses was studied in gastrointestinal content and tissue, peripheral plasma and urine using a multicompartmental non-targeted LC-MS approach in pigs. Both unsupervised and supervised multivariate analyses were used to study differences between the intervention groups, revealing significant effects of the dietary intervention on gastrointestinal contents and urine metabolites. Consumption of an AX rich diet composed of whole grain and enzyme treated wheat bran increased gastrointestinal content of novel sulfate- and acetic acid conjugated oxylipins, and urinary secretion of phenolic compounds. Furthermore, potential gastrointestinal and urinary biomarkers for consumption of resistant starch, whole grain rye, and a low dietary fiber intake were detected using multi block analysis. This study provides insight into microbial fermentation products in the gastrointestinal tract, and suggests a potential role in sulfate conjugation of metabolites on the bioavailability of ingested nutrients. In addition, the use of multicompartmental metabolomics offers information about the correlations between the compartments of the digestive system, providing additional insight into effects of increased whole grain intake.

AB - The effects of increased intake of dietary fiber as either arabinoxylan (AX) or resistant starch (RS) compared to a typical low dietary fiber Western-style diet (WSD) on the metabolomics responses was studied in gastrointestinal content and tissue, peripheral plasma and urine using a multicompartmental non-targeted LC-MS approach in pigs. Both unsupervised and supervised multivariate analyses were used to study differences between the intervention groups, revealing significant effects of the dietary intervention on gastrointestinal contents and urine metabolites. Consumption of an AX rich diet composed of whole grain and enzyme treated wheat bran increased gastrointestinal content of novel sulfate- and acetic acid conjugated oxylipins, and urinary secretion of phenolic compounds. Furthermore, potential gastrointestinal and urinary biomarkers for consumption of resistant starch, whole grain rye, and a low dietary fiber intake were detected using multi block analysis. This study provides insight into microbial fermentation products in the gastrointestinal tract, and suggests a potential role in sulfate conjugation of metabolites on the bioavailability of ingested nutrients. In addition, the use of multicompartmental metabolomics offers information about the correlations between the compartments of the digestive system, providing additional insight into effects of increased whole grain intake.

UR - http://pubs.acs.org/doi/abs/10.1021/acs.jproteome.5b00039

U2 - 10.1021/acs.jproteome.5b00039

DO - 10.1021/acs.jproteome.5b00039

M3 - Journal article

C2 - 26133160

SN - 1535-3893

VL - 14

SP - 3095

EP - 3110

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 8

ER -