VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule formation on Vicia sativa roots.

TY - JOUR

T1 - VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule formation on Vicia sativa roots.

AU - Vijn, I

AU - Yang, W C

AU - Pallisgård, N

AU - Jensen, E O

AU - van Kammen, A

AU - Bisseling, T

PY - 1995

Y1 - 1995

N2 - We isolated ENOD5, ENOD12 and ENOD40 homologues from Vicia sativa and studied their expression pattern during Rhizobium-induced nodule formation. Comparison of the VsENOD40 nucleotide sequence with the pea, soybean and alfalfa ENOD40 sequences showed that the sequences contain two conserved regions, called region I and region II. Comparison of all the potential open reading frames (ORFs) showed that all the five different ENOD40 clones encode a highly conserved small polypeptide of 12 or 13 amino acids encoded by an ORF located in region I. Furthermore we studied with in situ hybridization the expression pattern of VsENOD5, VsENOD12 and VsENOD40 during Rhizobium-induced nodule formation. Although the expression of these genes is largely similar to that of the pea counterparts, differences where found for the expression of VsENOD12 and VsENOD40 in Vicia. VsENOD12 is expressed in the whole prefixation zone II, whereas in pea ENOD12 is only expressed in the distal part of this zone. VsENOD40 is expressed in the uninfected cells of interzone II-III, while in pea ENOD40 is expressed in both the uninfected and infected cells of this zone. Udgivelsesdato: 1995-Sep

AB - We isolated ENOD5, ENOD12 and ENOD40 homologues from Vicia sativa and studied their expression pattern during Rhizobium-induced nodule formation. Comparison of the VsENOD40 nucleotide sequence with the pea, soybean and alfalfa ENOD40 sequences showed that the sequences contain two conserved regions, called region I and region II. Comparison of all the potential open reading frames (ORFs) showed that all the five different ENOD40 clones encode a highly conserved small polypeptide of 12 or 13 amino acids encoded by an ORF located in region I. Furthermore we studied with in situ hybridization the expression pattern of VsENOD5, VsENOD12 and VsENOD40 during Rhizobium-induced nodule formation. Although the expression of these genes is largely similar to that of the pea counterparts, differences where found for the expression of VsENOD12 and VsENOD40 in Vicia. VsENOD12 is expressed in the whole prefixation zone II, whereas in pea ENOD12 is only expressed in the distal part of this zone. VsENOD40 is expressed in the uninfected cells of interzone II-III, while in pea ENOD40 is expressed in both the uninfected and infected cells of this zone. Udgivelsesdato: 1995-Sep

KW - Amino Acid Sequence

KW - Fabaceae

KW - Gene Expression Regulation, Plant

KW - In Situ Hybridization

KW - Membrane Proteins

KW - Molecular Sequence Data

KW - Nitrogen Fixation

KW - Plant Proteins

KW - Plants, Medicinal

KW - RNA, Messenger

KW - Rhizobium

KW - Sequence Alignment

KW - Sequence Homology, Amino Acid

KW - Symbiosis

KW - Time Factors

M3 - Journal article

C2 - 7548828

SN - 0167-4412

VL - 28

SP - 1111

EP - 1119

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 6

ER -