Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome

TY - JOUR

T1 - Vitamin B12 and transcobalamin in bovine milk

T2 - Genetic variation and genome-wide association with loci along the genome

AU - Gebreyesus, Grum

AU - Poulsen, Nina Aagaard

AU - Larsen, Mette Krogh

AU - Larsen, Lotte Bach

AU - Sørensen, Esben Skipper

AU - Würtz Heegaard, Christian

AU - Buitenhuis, Albert Johannes

PY - 2021/5

Y1 - 2021/5

N2 - In human nutrition, bovine milk is an essential source of bioavailable vitamin B 12 and B 12-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B 12 and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectively. Additionally, we conducted whole-genome association analysis to identify SNP and genes associated with vitamin B 12 and transcobalamin. Our results indicated moderate to high heritability for vitamin B 12 (0.37 ± 0.18) and transcobalamin (0.61 ± 0.13) content in the Danish Holstein. With a significance threshold of –log 10 P-value > 5.87, significant associations were detected between SNP in Bos taurus autosome (BTA)17 and the log-transformed transcobalamin content of milk; no significant association was detected for vitamin B 12. The significant region in BTA17 was imputed to full sequence for further fine mapping, and the SNP with the most significant associations to transcobalamin were assigned to the transcobalamin 2 (TCN2) gene.

AB - In human nutrition, bovine milk is an essential source of bioavailable vitamin B 12 and B 12-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B 12 and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectively. Additionally, we conducted whole-genome association analysis to identify SNP and genes associated with vitamin B 12 and transcobalamin. Our results indicated moderate to high heritability for vitamin B 12 (0.37 ± 0.18) and transcobalamin (0.61 ± 0.13) content in the Danish Holstein. With a significance threshold of –log 10 P-value > 5.87, significant associations were detected between SNP in Bos taurus autosome (BTA)17 and the log-transformed transcobalamin content of milk; no significant association was detected for vitamin B 12. The significant region in BTA17 was imputed to full sequence for further fine mapping, and the SNP with the most significant associations to transcobalamin were assigned to the transcobalamin 2 (TCN2) gene.

UR - http://www.scopus.com/inward/record.url?scp=85148316568&partnerID=8YFLogxK

U2 - 10.3168/jdsc.2020-0048

DO - 10.3168/jdsc.2020-0048

M3 - Journal article

C2 - 36339496

SN - 2666-9102

VL - 2

SP - 127

EP - 131

JO - JDS Communications

JF - JDS Communications

IS - 3

ER -