Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis

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  • Pune Nina Tawakoli, Clinic of Preventive Dentistry, Periodontology and Cardiology, Centre of Dental Medicine, University of Zurich, Switzerland, Schweiz
  • Thomas R Neu, Department of River Ecology, Helmholtz Centre for Environmental Research – UFZ, Magdeburg, Germany, Tyskland
  • Mette Marie Busck
  • Ute Kuhlicke, Department of River Ecology, Helmholtz Centre for Environmental Research - UFZ, Magdeburg, Germany.
  • ,
  • Andreas Schramm
  • Thomas Attin, Clinic of Preventive Dentistry, Periodontology and Cardiology, Centre of Dental Medicine, University of Zurich, Switzerland, Schweiz
  • Daniel B Wiedemeyer, Statistical Services, Centre of Dental Medicine, University of Zurich, Switzerland, Schweiz
  • Sebastian Schlafer
The extracellular matrix is a poorly studied, yet important component of dental biofilms. Fluorescence lectin-binding analysis (FLBA) is a powerful tool to characterize glycoconjugates in the biofilm matrix. This study aimed to systematically investigate the ability of 75 fluorescently labeled lectins to visualize and quantify extracellular glycoconjugates in dental biofilms. Lectin binding was screened on pooled supragingival biofilm samples collected from 76 subjects using confocal microscopy. FLBA was then performed with 10 selected lectins on biofilms grown in situ for 48 h in the absence of sucrose. For five lectins that proved particularly suitable, stained biovolumes were quantified and correlated to the bacterial composition of the biofilms. Additionally, combinations of up to three differently labeled lectins were tested. Of the 10 lectins, five bound particularly well in 48-h-biofilms: Aleuria aurantia (AAL), Calystega sepiem (Calsepa), Lycopersicon esculentum (LEA), Morniga-G (MNA-G) and Helix pomatia (HPA). No significant correlation between the binding of specific lectins and bacterial composition was found. Fluorescently labeled lectins enable the visualization of glycoconjugates in the dental biofilm matrix. The characterization and quantification of glycoconjugates in dental biofilms require a combination of several lectins. For 48-h-biofilms grown in absence of sucrose, AAL, Calsepa, HPA, LEA, and MNA-G are recommendable.
OriginalsprogEngelsk
TidsskriftJournal of Oral Microbiology
Vol/bind9
Nummer1
ISSN2000-2297
DOI
StatusUdgivet - 9 jul. 2017

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