TY - JOUR
T1 - Visible Light Neural Stimulation on graphitic-Carbon Nitride/Graphene Photocatalytic Fibers
AU - Zhang, Zhongyang
AU - Xu, Ruodan
AU - Wang, Zegao
AU - Dong, Mingdong
AU - Cui, Bianxiao
AU - Chen, Menglin
PY - 2017
Y1 - 2017
N2 - Light stimulation allows remote and spatiotemporally accurate operation that has been applied as effective, noninvasive means of therapeutic interventions. Here, visible-light neural stimulation of graphitic carbon nitride (g-C
3N
4), an emerging photocatalyst with visible-light optoelectronic conversion, was for the first time investigated. Specifically, g-C
3N
4 was combined with graphene oxide (GO) in a three-dimensional manner on the surfaces of electrospun polycaprolactone/gelatin (PG) fibers and functioned as a biocompatible interface for visible-light stimulating neuronal differentiation. The enhanced photocatalytic function of g-C
3N
4 was realized by spreading g-C
3N
4 on GO coated electrospun (PG) microfibers to improve both charge separation and surface area. Ascorbic acid (AA) was used in the cell culture medium not only as a photoexcited hole scavenger but also as a mediator of GO reduction to further improve the electrical conductivity. The successful coatings of g-C
3N
4, GO, and AA-mediated GO reduction were confirmed using scanning electron microscopy, photoluminescence, Raman spectroscopy, and X-ray photoelectron spectroscopy. Biocompatibility of g-C
3N
4 (0.01-0.9 mg/mL) to PC12 cells was confirmed by the lactate dehydrogenase (LDH) assay, Live-Dead staining, and colorimetric cell viability assay CCK-8. Under a bidaily, monochromatic light stimulation at a wavelength of 450 nm at 10 mW/cm
2, a 18.5-fold increase of neurite outgrowth of PC12 was found on g-C
3N
4-coated fibers, while AA-reduced GO-g-C
3N
4 hybrid brought a further 2.6-fold increase, suggesting its great potential as a visible-light neural stimulator that could optically enhance neural growth in a spatiotemporal-specific manner.
AB - Light stimulation allows remote and spatiotemporally accurate operation that has been applied as effective, noninvasive means of therapeutic interventions. Here, visible-light neural stimulation of graphitic carbon nitride (g-C
3N
4), an emerging photocatalyst with visible-light optoelectronic conversion, was for the first time investigated. Specifically, g-C
3N
4 was combined with graphene oxide (GO) in a three-dimensional manner on the surfaces of electrospun polycaprolactone/gelatin (PG) fibers and functioned as a biocompatible interface for visible-light stimulating neuronal differentiation. The enhanced photocatalytic function of g-C
3N
4 was realized by spreading g-C
3N
4 on GO coated electrospun (PG) microfibers to improve both charge separation and surface area. Ascorbic acid (AA) was used in the cell culture medium not only as a photoexcited hole scavenger but also as a mediator of GO reduction to further improve the electrical conductivity. The successful coatings of g-C
3N
4, GO, and AA-mediated GO reduction were confirmed using scanning electron microscopy, photoluminescence, Raman spectroscopy, and X-ray photoelectron spectroscopy. Biocompatibility of g-C
3N
4 (0.01-0.9 mg/mL) to PC12 cells was confirmed by the lactate dehydrogenase (LDH) assay, Live-Dead staining, and colorimetric cell viability assay CCK-8. Under a bidaily, monochromatic light stimulation at a wavelength of 450 nm at 10 mW/cm
2, a 18.5-fold increase of neurite outgrowth of PC12 was found on g-C
3N
4-coated fibers, while AA-reduced GO-g-C
3N
4 hybrid brought a further 2.6-fold increase, suggesting its great potential as a visible-light neural stimulator that could optically enhance neural growth in a spatiotemporal-specific manner.
KW - Journal Article
UR - http://www.scopus.com/inward/record.url?scp=85031104976&partnerID=8YFLogxK
U2 - 10.1021/acsami.7b12733
DO - 10.1021/acsami.7b12733
M3 - Journal article
C2 - 28929741
SN - 1944-8244
VL - 9
SP - 34736
EP - 34743
JO - A C S Applied Materials and Interfaces
JF - A C S Applied Materials and Interfaces
IS - 40
ER -