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Viability determination of Bacillus sphaericus after encapsulation in hydrogel for self-healing concrete via microcalorimetry and in situ oxygen concentration measurements

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  • Xuejiao Zhu, Ghent University - Department of Structural Engineering and Building Materials
  • ,
  • Arn Mignon, Ghent University - Department of Organic and Macromolecular Chemistry, KU Leuven
  • ,
  • Søren Dollerup Nielsen
  • ,
  • Silvia Elisabeth Zieger
  • Klaus Koren
  • Nico Boon, Ghent University, Belgien
  • Nele De Belie, Ghent University - Department of Structural Engineering and Building Materials, Danmark
Techniques for bacteria based self-healing concrete are currently limited to indirect bacterial activity measurements and visual crack closing effects after self-healing has occurred. In the present study, direct and reliable bacteria survival measurements after encapsulation in protective carriers were carried out by utilizing microcalorimetry analysis. After further incorporation in mortar oxygen consumption was determined by use of an optical oxygen sensing system. The microscope analysis showed that a 90% sporulation of Bacillus sphaericus was achieved after 24 h incubation. Microcalorimeter measurements detected the heat generation from microbial activity on the third day of incubation which provides direct evidence that bacteria can be activated after encapsulation inside the hydrogel. The application of the O2 sensing film on cracked mortar showed a high oxygen concentration reduction (66%) near the crack opening. This confirmed the activation of B. sphaericus and use of oxygen as an electron acceptor for their metabolism after incorporation inside mortar specimens.
OriginalsprogEngelsk
Artikelnummer104006
TidsskriftCement and Concrete Composites
Vol/bind119
ISSN0958-9465
DOI
StatusUdgivet - maj 2021

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