TY - JOUR
T1 - Validation of a surgical model for posthepatectomy liver failure in rats
AU - Lund, Andrea
AU - Meier, Michelle
AU - Andersen, Kasper Jarlhelt
AU - Pedersen, Marie Ingemann
AU - Knudsen, Anders Riegels
AU - Kirkegård, Jakob
AU - Mortensen, Frank Viborg
PY - 2023/6
Y1 - 2023/6
N2 - Background: The upper limit for liver resections in rats is approximately 90%. In the early postoperative phase, mortality increases. The aim of the present study was to validate the rat model of 90% partial hepatectomy (PH) as a model of post-hepatectomy liver failure (PHLF). Further, we wanted to test a quantitative scoring system as a detector of lethal outcomes caused by PHLF in rats. Methods: Sixty-eight rats were randomized to 90% PH, sham operation, or no surgery. Further, block randomization was performed based on time of euthanization: 12, 24, or 48 h after surgery. A general distress score (GDS) ≥10 during the day or ≥6 at midnight prompted early euthanization and classification as nonsurvivor. Animals euthanized as planned were classified as survivors. During euthanization, blood and liver tissue were collected, and liver-specific biochemistry was evaluated. Results: Based on the biochemical results, all animals subjected to 90% PH experienced PHLF. Seventeen rats were euthanized due to irreversible PHLF. The GDS increased for nonsurvivors within 12–18 h after surgery. The mean time for euthanization was 27 h after surgery. Conclusion: Based on the GDS and liver-specific biochemistry, we concluded that the model of 90% PH seems to be a proper model for investigating PHLF in rats. As a high GDS is associated with increased mortality, the GDS appears to be valuable in detecting lethal outcomes caused by PHLF in rats.
AB - Background: The upper limit for liver resections in rats is approximately 90%. In the early postoperative phase, mortality increases. The aim of the present study was to validate the rat model of 90% partial hepatectomy (PH) as a model of post-hepatectomy liver failure (PHLF). Further, we wanted to test a quantitative scoring system as a detector of lethal outcomes caused by PHLF in rats. Methods: Sixty-eight rats were randomized to 90% PH, sham operation, or no surgery. Further, block randomization was performed based on time of euthanization: 12, 24, or 48 h after surgery. A general distress score (GDS) ≥10 during the day or ≥6 at midnight prompted early euthanization and classification as nonsurvivor. Animals euthanized as planned were classified as survivors. During euthanization, blood and liver tissue were collected, and liver-specific biochemistry was evaluated. Results: Based on the biochemical results, all animals subjected to 90% PH experienced PHLF. Seventeen rats were euthanized due to irreversible PHLF. The GDS increased for nonsurvivors within 12–18 h after surgery. The mean time for euthanization was 27 h after surgery. Conclusion: Based on the GDS and liver-specific biochemistry, we concluded that the model of 90% PH seems to be a proper model for investigating PHLF in rats. As a high GDS is associated with increased mortality, the GDS appears to be valuable in detecting lethal outcomes caused by PHLF in rats.
KW - 90% liver resection
KW - general distress score
KW - liver failure
KW - post-hepatectomy liver failure
KW - rats
UR - http://www.scopus.com/inward/record.url?scp=85159175495&partnerID=8YFLogxK
U2 - 10.1002/ame2.12325
DO - 10.1002/ame2.12325
M3 - Journal article
C2 - 37183349
AN - SCOPUS:85159175495
SN - 2096-5451
VL - 6
SP - 266
EP - 273
JO - Animal Models and Experimental Medicine
JF - Animal Models and Experimental Medicine
IS - 3
ER -