User Guide for the LORE1 Insertion Mutant Resource

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskning


Lotus japonicus is a model legume used in the study of plant-microbe interactions, especially in the field of biological nitrogen fixation due to its ability to enter into a symbiotic relationship with a soil bacterium, Mesorhizobium loti. The LORE1 mutant population is a valuable resource for reverse genetics in L. japonicus due to its non-transgenic nature, high tagging efficiency, and low copy count. Here, we outline a workflow for identifying, ordering, and establishing homozygous LORE1 mutant lines for a gene of interest, LjFls2, including protocols for growth and genotyping of a segregating LORE1 population.

TitelPlant Genomics. Methods in Molecular Biology
Antal sider11
ForlagHumana Press
ISBN (trykt)978-1-4939-7001-8
ISBN (Elektronisk)978-1-4939-7003-2
StatusUdgivet - 2017
SerietitelMethods in molecular biology (Clifton, N.J.)

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