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Use of a culture independent method to analyze the diversity of soil fungi surrounding Chroogomphus rutilus in the Beijing region of China

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  • Yu Liu, Beijing Academy of Agriculture and Forestry Science, Kina
  • Shouxian Wang, Beijing Academy of Agriculture and Forestry Science, Kina
  • Yonggang Yin, Beijing Academy of Agriculture and Forestry Science, Kina
  • Haojie Jin, Danmark
  • Shuang Zhao, Beijing Academy of Agriculture and Forestry Science, Kina
  • Ming Li, Agricultural University of Hebei, Kina
  • Peng Wang, Beijing Academy of Agriculture and Forestry Science, Kina
  • Xiaoli Geng, Beijing Academy of Agriculture and Forestry Science, Kina
  • Feng Xu, Beijing Academy of Agriculture and Forestry Science, Kina
Large-scale cultivation of Chroogomphus rutilus is too inefficient to be commercially feasible. In addition, isolating C. rutilus mycelia in the wild is difficult. Thus, determining the natural habitat of its fruiting body is important. The present study focused on the ecology of the C. rutilus habitat to facilitate its large-scale cultivation. A culture-independent molecular approach—a powerful technology for microbiological ecology studies—was used to investigate the diversity of soil fungal communities in samples surrounding C. rutilus from the Beijing region of China. Metagenomic DNA was isolated from soil samples collected around C. rutilus, and an internal transcribed spacer (ITS) gene library was constructed. Subsequently, polymerase chain reaction products were digested with HinfI, HaeIII, MspI, TaqI, or MboI. Clones were selected and sequenced based on their restriction fragment length polymorphisms. The diversity of the fungi represented by their ITS sequences was analyzed. Our results indicate the presence of numerous fungi in the C. rutilus habitat. This study is the first demonstration of the fungal ecology surrounding C. rutilus using a culture independent method
OriginalsprogEngelsk
TidsskriftAnnals of Microbiology
Vol/bind62
Nummer4
Sider (fra-til)1743-1749
Antal sider7
ISSN1590-4261
DOI
StatusUdgivet - 1 dec. 2012

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